How can we make the primary research literature more accessible to naive readers such as undergraduate students?
Some of my ideas and strategies are summarized in an unpublished manuscript
Biology Journal Club for Engineering Students. A shorter summary is available in the form
of a handout from
the 2005 Scholarship of Teaching and Learning Symposium at the University
Central to my approach is the creation of detailed study guides designed
with confused students in mind. Links to some of these study guides (and
the papers on which they are based) can be found below and on the BIOL 485 web page.
* * * * *
C.M. Armstrong and D.E. Goldberg, "An FKBP destabilization domain modulates protein levels in Plasmodium falciparum,"
Nature Methods 4: 1007-9, 2007.
PubMed ID #17994030;
C. Aslanidis & P.J. de Jong, "Ligation-independent cloning of PCR products (LIC-PCR),"
Nucleic Acids Research 18: 6069-74, 1990.
PubMed ID #2235490;
J. Baldwin et al., "High-throughput screening for potent and selective inhibitors of Plasmodium falciparum dihydroorotate dehydrogenase,"
Journal of Biological Chemistry 280: 21847-53, 2005.
PubMed ID #15795226;
M.L. Baniecki et al., "High-throughput Plasmodium falciparum growth assay for malaria drug discovery,"
Antimicrobial Agents and Chemotherapy 51: 716-23, 2007.
PubMed ID #17116676;
F.S. Buckner and J.A. Urbina, "Recent developments in sterol 14-demethylase inhibitors for Chagas disease,"
International Journal for Parasitology: Drugs and Drug Resistance 2: 236-42, 2012.
PubMed ID #23277882;
L.A. Castle et al., "Discovery and directed evolution of a glyphosate tolerance gene,"
Science 304: 1151-4, 2004.
PubMed ID #15155947;
T.D.Y. Chung and D.J. Murphy, "Enzyme screens," from
Handbook of Drug Screening (R. Seethala and P.B. Fernandes, eds.), 2001.
Google Books link;
G.J. Crowther et al., "Buffer optimization of thermal melt assays of Plasmodium proteins for detection of small-molecule
Journal of Biomolecular Screening 14: 700-7, 2009.
PubMed ID #19470714;
M.B. Elowitz and S. Leibler, "A synthetic oscillatory network of transcriptional regulators,"
Nature 403: 335-338, 2000.
PubMed ID #10659856;
I. Faik et al., "Parasite-host interaction in malaria: genetic clues and copy number variation,"
Genome Medicine 1: 82, 2009.
PubMed ID #19725943;
W. Gilbert and B. Muller-Hill, "The lac operator is DNA,"
Proceedings of the National Academy of Sciences USA
58: 2415-2421, 1967.
PubMed ID #4873589;
S.H. Grossman, "A note on the dual role of glucose in the protection of glucokinase against inactivation,"
Biochimica et Biophysica Acta 452: 392-7, 1976.
PubMed ID #1009116;
R. Gujjar et al., "Identification of a metabolically stable triazolopyrimidine-based dihydroorotate dehydrogenase inhibitor with
antimalarial activity in mice,"
Journal of Medicinal Chemistry 52: 1864-72, 2009.
PubMed ID #19296651;
V.M. Ingram, "Gene mutations in haemoglobin: the chemical difference between normal and
sickle-cell haemoglobin," Nature 180: 326-328, 1957.
PubMed ID #13464827;
E.R. Kandel, "Calcium and the control of synaptic strength by learning,"
Nature 293: 697-700, 1981.
PubMed ID #7290207;
J.X. Kelly et al., "Discovery of dual function acridones as a new antimalarial chemotype,"
Nature 459: 270-3, 2009.
PubMed ID #19357645;
E.T. Larson et al., "Toxoplasma gondii cathepsin L is the primary target of the invasion-inhibitory compound
morpholinurea-luecyl-homophenyl-vinyl sulfone phenyl,"
Journal of Biological Chemistry 284: 26839-50, 2009.
PubMed ID #19596863;
K.P. Lemon and A.D. Grossman, "Localization of bacterial DNA polymerase: evidence for a factory model of replication,"
Science 282: 1516-1519, 1998.
PubMed ID #9822387;
S.L. Lindstedt et al., "Limitations to aerobic performance in mammals: interaction of structure and demand,"
International Journal of Sports Medicine 9: 210-217, 1988.
PubMed ID #3410627;
H.-R. Luscher et al., "How the size of motoneurones determines their susceptibility to discharge,"
Nature 282: 859-861, 1979.
PubMed ID #229426;
G.J. Lutz and L.C. Rome, "Built for jumping: the design of the frog muscular system,"
Science 263: 370-372, 1994.
PubMed ID #8278808;
P.J. Myler et al., "The Seattle Structural Genomics Center for Infectious Disease (SSGCID),"
Infectious Disorders - Drug Targets 9: 493-506, 2009.
PubMed ID #19594426;
M.A. Phillips et al., "Triazolopyrimidine-based didhydroorotate dehydrogenase inhibitors with potent and selective activity
against the malaria parasite Plasmodium falciparum,"
Journal of Medicinal Chemistry 51: 3649-53, 2008.
PubMed ID #18522386;
M. Rottmann et al., "Spiroindolones, a potent compound class for the treatment of malaria,"
Science 329: 1175-80, 2010.
PubMed ID #20813948;
F. Sanger et al., "DNA sequencing with chain-terminating inhibitors,"
Proceedings of the National Academy of Sciences USA 74: 5463-5467, 1977.
PubMed ID #271968;
J.G. Swoboda et al., "Discovery of a small molecule that blocks wall teichoic acid biosynthesis in
ACS Chemical Biology 4: 875-83, 2009.
PubMed ID #19689117;
M. Tamborrini et al., "Design and pre-clinical profiling of a Plasmodium falciparum MSP-3 derived component for a multi-valent
virosomal malaria vaccine,"
Malaria Journal 8: 314, 2009.
PubMed ID #20042100;
H.M. Taylor et al., "The malaria parasite cyclic GMP-dependent protein kinase plays a central role in
Eukaryotic Cell 9: 37-45, 2010.
PubMed ID #19915077;
M. Vedadi et al., "Chemical screening methods to identify ligands that promote protein stability,
protein crystallization, and structure determination,"
Proc. Natl. Acad. Sci. USA 103: 15835-40, 2006.
Pub Med ID #17035505;
X.-T. Wang and P.C. Engel, "Clinical mutants of human glucose 6-phosphate dehydrogenase: impairment of NADP(+) binding affects both folding and stability,"
Biochimica et Biophysica Acta 1792: 804-9, 2009.
PubMed ID #19465117;