This is a collaborative work with the Fliesler lab at SUNY-Buffalo titled “Prevention of Retinal Degeneration in a Rat Model of Smith-Lemli-Opitz Syndrome“. This work represents the first successful prevention of a SLOS phenotype, retinal degeneration, in an animal model! Congrats, Josi and Kelly!
Our collaborative work with the Werth Lab in the Department of Pharmacy and the Salipante Lab in the Department of Laboratory Medicine was published in mSphere! This work reports characterization of the lipidomic changes associated with daptomycin resistance in three Gram-positive pathogens, E. faecalis, S. aureus, and C. striatum, and correlation with their genetic changes. Read the paper here.
Our paper on the measurement of collision cross sections (CCS) of over 1400 drug and drug-like molecules using traveling wave IM-MS was accepted to Analytical Chemistry! Congratulations to Kelly and Dylan, and our collaborators at the Department of Chemistry, Kim Davidson and Matt Bush! Great team effort!
Our collaborative work titled: “Enzymatic and Free Radical Formation of Cis- and Trans- Epoxyeicosatrienoic Acids In Vitro and In Vivo” in collaboration with the Totah Lab was accepted for publication in the journal Free Radical Biology and Medicine. This is the first demonstration that both trans and cis- epoxyeicosatrienoic acids (EETs) can be formed from arachidonic acid under free radical oxidation condition.
Congratulations to Kelly and Josi on their paper “Assessment of Altered Lipid Homeostasis by HILIC-Ion Mobility-Mass Spectrometry-Based Lipidomics” being accepted to the Journal of Lipid Research! This lipidomics methodology was developed based on the orthogonal HILIC and ion mobility separations, which enables lipid identification by lipid class polarity and collision cross sections (CCS) of individual species. The method was demonstrated by elucidating the effect of small molecules on lipid homeostasis in neuronal cells.
This is a collaboration with Prof. Carrie Finno at UC – Davis. We contributed to this work on the oxysterol analysis! Congrats to Josi, David, and Kelly! Here is the link to the paper.
This paper established a set of calibrants for accurate determination of lipid collision cross section using traveling Wave Ion Mobility-Mass Spectrometry, which would serve as the foundation for multi-dimensional lipidomics studies. Congrats, Kelly! Check out this paper here.
This collaboration with the laboratory of Dr. Philip Beachy reported identification of 7-dehydrocholesterol-derived oxysterols as antagonists of Hedgehog signaling, specifically 5,8-endoperoxide and DHCEO! Read the paper here.
Congrats to Josi, our former member, Rosalyn Reese, and the rest of the team on their wonderful work! This marks the first paper from our lab at UW! This paper reports the identification of quaternary ammonium compounds as potent inhibitors of Dhcr7, the same enzyme that was affected in SLOS. Check out our publication here.
This collaborative work with Dr. Steven Fliesler at SUNY-Buffalo recently published on Experimental Eye Research found that photoreceptor-derived cells are the most sensitive cells to the insult of 7-DHC oxysterols. Check out the paper here.