Microtubule nucleation by γ-tubulin complexes
Prof. Kollman’s postdoctoral work focused on the structures of γ-tubulin complexes. This work clarified the mechanism of microtubule nucleation, in which γ-tubulin is presented in a ring to serve as a template for microtubule growth via longitudinal contact with αβ-tubulin. The structure of oligomers of the γ-tubulin small complex (γTuSC) suggested that nucleation activity is regulated by a conformational change in a specific γTuSC component. Continuing work is focused on elucidating the details of this conformational change, and determining the structure of γTuSC interacting with microtubules.
Modes of γ-TuSC and γ-TuRC-specific attachment
1959 NE Pacific Street Box 357350
Seattle, WA 98195