Plasmodium proteins

Below is a list of purified, concentrated Plasmodium proteins currently in UW freezers and available for thermal melt assays, enzyme activity assays, etc. Total proteins as of July 24th, 2009: 68.

Protein name/function	PlasmoDB ID
2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	PVX_003920
6-phosphogluconolactonase	PF14_0511
6-pyruvoyltetrahydropterin synthase -- P. falciparum	PFF1360w
6-pyruvoyltetrahydropterin synthase -- P. vivax	PVX_114505
Adenosine deaminase (ADA) -- P. falciparum	PF10_0289
Adenosine deaminase (ADA) -- P. vivax	PVX_111245
Adenylosuccinate lyase	PVX_003765
Adenylosuccinate synthetase	PF13_0287
Aspartate carbamoyltransferase	PVX_083135
ATP-dependent CLP protease	PFC0310c
Branched-chain alpha keto-acid dehydrogenase	PF13_0070
CDK-related protein kinase 6 (PK6)	MAL13P1.185
Choline kinase	PF14_0020
Cyclophilin	PFE1430c
D-ribulose-5-phosphate 3-epimerase	PFL0960w
Deoxyribose-phosphate aldolase	PY02252
DNA helicase	PFI0910w
dUTPase	PF11_0282
Dynein light chain 1	PFL0660w
Eukaryotic translation initiation factor 5a (eIF5a)	PFL0210c
Farnesyl pyrophosphate synthase	PVX_092040
Glutamate dehydrogenase (NADP-specific)	PF14_0164
Glyceraldehyde-3-phosphate dehydrogenase	PF14_0598
Glycerol-3-phosphate dehydrogenase	PFL0780w
Glycogen synthase kinase 3 (GSK)	PFC0525c
Glyoxalase I	PF11_0145
Guanylate kinase	PVX_099895
Hydroxymethyldihydropterin pyrophosphokinase	PVX_123230
Hypothetical protein, conserved	MAL13P1.257
Hypothetical protein, conserved	PFF0880c
Methionine adenosyltransferase (a.k.a. SAM synthetase)	PFI1090w
Methionine aminopeptidase 1	PF10_0150
N-myristoyltransferase	PF14_0127
Nucleoside diphosphate kinase B	PF13_0349
Nucleosome assembly protein 1	PKH_130240
Ornithine aminotransferase	PY00104
Orotidine 5' monophosphate decarboxylase (OMPDC)	PF10_0225
Peptidase, putative	PF14_0517
Phosphatidylethanolamine-binding protein	PVX_123630
Phosphoethanolamine N-methyltransferase (PENMT)	MAL13P1.214
Phosphoglycerate kinase (PGK)	PFI1105w
Phosphoglycerate mutase (PGM)	PF11_0208
Phosphomethylpyrimidine kinase (PMPK)	PFE1030c
Protein-L-isoaspartate O-methyltransferase	PF14_0309
Pyrroline carboxylate reductase	MAL13P1.284
Rab11 GTPase	PF13_0119
Rab18 GTPase	PF08_0110
Ribonucleotide reductase, small subunit	PVX_086155
Ribose 5-phosphate isomerase	PFE0730c
Ribosomal RNA methyltransferase	PF13_0052
S-Adenosyl-homocysteine hydrolase (SAHH)	PFE1050w
Spermidine synthase	PF11_0301
Superoxide dismutase (SOD) -- P. berghei	PB000490.02.0
Superoxide dismutase (SOD) -- P. knowlesi	PKH_142350
Thioredoxin	PF14_0545
Thioredoxin	MAL13P1.159
Thioredoxin peroxidase	PFL0725w
Thioredoxin reductase	PFI1170c
Tryptophan tRNA ligase	PF13_0205
Ubiquitin carboxyl-terminal hydrolase	PF14_0576
Ubiquitin conjugating enzyme	PF10_0330
Ubiquitin conjugating enzyme	PFE1350c
Ubiquitin conjugating enzyme	PF13_0301
Ubiquitin conjugating enzyme	PF08_0085
Ubiquitin conjugating enzyme	PFC0255c
Ubiquitin conjugating enzyme	PFL0190w
Uridine phosphorylase (purine nucleoside phosphorylase)	PFE0660c
Vacuolar protein-sorting protein	PF08_0064

Notes on selected proteins that are not currently available or useful....

Protein name/function	PlasmoDB ID	Comments
5-aminolevulinate synthase	PKH_146340	The protein labeled as 5-aminolevulinate synthase turned out (according to sequencing data) to be aminomethyltransferase from L. naiffi.
Dihydrofolate synthase/folylpolyglutamate synthase	PF13_0140	Protein prep looks pure but gives poor melting curves.
DNA helicase	PF13_0308	Bad melting curves.
Dual-specificity protein phosphatase	PFC0380w	Bad melting curves.
Eukaryotic translation initiation factor 2b (eIF2b), alpha subunit	PF08_0009	Bad melting curves.
HSP90	PF11_0188	Bad melting curves.
Hydroxymethyldihydropterin pyrophosphokinase	PVX_123230	Bad melting curves.
Hypothetical protein, conserved	PFL2235w	This protein turned out (according to sequencing data) to be a ubiquitin conjugating enzyme (PFL0190w).
Nucleolar rRNA processing protein EBP2	PFL2235w	This protein turned out (according to sequencing data) to be a hypothetical protein (PFF0880c).
Pyrroline carboxylate reductase	MAL13P1.284	Crashed out of solution during concentration.
Serine/threonine protein kinase	PF13_0085	The protein labeled as serine/threonine protein kinase turned out (according to sequencing data) to be eTIF5a from Leishmania.