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Thermal Melt With You

This parody (written by Greg Crowther) is sung to the tune of "I Melt With You" (written and performed by Modern English).


Lyrics

Youíre a protein from a pathogen,
And Iím a scientist who knows your T-sub-m.
Youíre in solution, and youíre tumbling all around,
But my new method will heat you up and break you down.

Iíll start a thermal melt with you;
I see a difference in stability when ligands bind.
Since 3D modeling wonít do,
Iíll start a thermal melt with you.

To raise my throughput, I use a protocol
First introduced by the team of Pantoliano et al.
Most compounds donít bind, but picture my delight
At a well where your melting curve is shifted to the right!

Iíll start a thermal melt with you;
I see a difference in stability when ligands bind.
Iíll find the ones that stick like glue;
Iíll start a thermal melt with you.

Your pocketís open wide....

Iíll start a thermal melt with you;
I see a difference in stability when ligands bind.
This works with other proteins too, but
Iíll start a thermal melt with you.

Your pocketís open wide....

Iíll start a thermal melt with you;
I see a difference in stability when ligands bind.
Since 3D modeling wonít do,
Iíll start a thermal melt with you.

Iíll start a thermal melt with you.
Iíll start a thermal melt with you.
Iíll start a thermal melt with you.
Iíll start a thermal melt with you.


Comments

Thermal melting -- also known as Differential Scanning Fluorimetry (DSF) and other names -- is a method used to identify compounds that bind to and stabilize a protein of interest, and thus raise its melting temperature (Tm). Its feasibility as a high-throughput screening method was first demonstrated in a 2001 paper by Pantoliano et al.

The line "Since 3D modeling won't do" refers to the relatively poor performance of structural models in predicting which compounds will bind to a protein. The line "Your pocket's open wide" refers to an active site or other pocket where a compound could conceivably bind.

This song was written for one of my favorite audiences: the biotechnology students at Glacier Peak High School who annually visit the University of Washington.