Keller Lab Links

last edited (haphazardly) 2016
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Reserved for Keller Lab:

How to Attend Group Meetings

  • Location: CHB239 Tuesdays 2:00-3:30pm
  • How to propose to change the group meeting time/place:
    1) Ask a secretary if a conference room is available at your time (in order of preference: CHB239, 339, 439, BAG319)
    2) Set up a 5-day chart at with the times that you and the rooms are available.
    3) E-mail amphiphiliphiles - ask them to enter their schedules. Send reminders. Verify that you can still book the room.
    4) Inform everyone of the change. Send reminders before the meeting to help old dogs learn new tricks.

How to Buy/Build Things & Learn about Membrane Biophysics

How to Use and Maintain Microscopes

  • START HERE: Nikon's Microscopy U
  • TECHNIQUE: Procedure for changing lamp in Nikon microscope (.pdf)
  • TECHNIQUE: At the end of the travel on the fine focus knob? Back up and use coarse focus, then fine again. New lab members commonly ruin the fine focus by overtwisting. For example, if the lubrication inside is too old, then the fine focus will act as if it is fused with the coarse focus.
  • TECHNIQUE: Micromanager, a free ImageJ plug-in to control microscope cameras
  • TECHNIQUE: Many of our lenses have correction collars. Adjusting them properly for the cover slip you use makes a big difference.
  • TECHNIQUE:Is the lens stuck? Use a rubber strap wrench, not a metal wrench.
  • BUY: Lubricate microscopes with "Nyoil II" to lubricate microscopes
  • BUY: "Packing oil" to lubricate microscope adjusting screws, because it stays in place.
  • BUY: "Sparkle" glass cleaner to clean lenses and objectives (any department store), or "Formula MD" lens cleaner. Ethanol/isopropanol may eventually dissolve the glue holding the lens in place.
  • BUY: Photodiode light sources: Jan Strelow recommends Chris Baumann at 89 North or perhaps AMG (advanced microscopy group)
  • BUY: Filter cubes. Jan Strelow recommends Chroma, inform that have older metal cubes in which filters have problems and want new hard coats.

How to Complete Yearly IDP Forms, Mentor-Mentee Expectations Checklists, and Compacts

User Facilities and Makerspaces

  • UW Makerspace (appears to be free except for consumables)
  • Metrix Maker Space on Capitol Hill - electronics, laser cutter, 3D printer, sewing machine, classes... and coffee! Membership and walk-ins.
  • UW Analyltical Biopharmacy Core with ultracentrifuge, DSC, ITC, SPR, viscometer, etc. Apply for matching funds up to $5K/lab.

Data Management, Statistics, Computing, and Programs

Other Links for Keeping the Keller Lab Functioning

Conferences / Travel / Posters / Talks

  • MEMBERSHIP: APS (specify DBIO, Division of Biological Sciences)
  • MEMBERSHIP: Biophysical Society (specify the MSAS subgroup)
  • CONFERENCES: Worldwide Biophysics Conferences, and also through the EBSA, HERE
  • CONFERENCES - BIOPHYSICAL SOCIETY: [1] Become a member or renew. To find your member #, search e-mail for "membership receipt". Choose student membership. Upload copy of student ID with SLK's signature. [2] From main page, click "submit an abstract" and log in. [3] Select "poster only" if competing in SRAA competition, which you should if a student. Typical topic #1:"3A: Membrane physical chemistry", #2 "3E: Membrane structure". [4] Select "student member combo fee" (which means you are paying for the conference registration at the same time as the abstract fee) and pay with credit card. Fill out the Chem online travel request form. If you have questions, see the information and FAQs here. Ariba will e-mail, requiring verification of reimbursement. [5] Go to the website for this year's BPS meeting. There should be a tab for "Awards & Competitions" that leads to a link to "Poster Competition". The form will ask for your "Abstract Control Number", which you can find in the receipt that the BPS sent you after you paid for the submission of your abstract. The number is inside the "Product Name" box. You have to select a 1st and 2nd choice for the subgroup. For almost everyone in our group, you should select "Membrane Structure & Assembly" for both! [6] If you do not receive notification that you are enrolled in the poster competition (but want to be), e-mail the BPS! SNAFUs happen!
  • TRAVEL: UW requirements for international travel of students and departmental rules for travel reimbursements.
  • TRAVEL: In order to be reimbursed for travel, all students, postdocs and staff must be pre-approved BEFORE traveling! First, fill out the online form for prior approval for either Students/Postdocs/Staff or Faculty. Then fill out a separate paper form that you get from the Purchasing Office, (or you can print out the separate faculty form HERE and cross out "faculty".) Fill out the paper form, have Sarah sign it (anywhere... there's no line on the form), and submit it in person to Purchasing upstairs. You can have the staff buy your plane ticket at that same time. You can also get an advance on your travel budget if you need one. You can also arrange for the department to pay your hotel bill directly if you do so about a week in advance.
  • TRAVEL: If you have passed your 3rd Year exam, request a travel award from your dept. If you receive one from the Chem Dept, forward our group's administrative staff person (currently Madison Canfield) the e-mail that announced your award so that the travel funds can be used to reimburse you. Current Chem grad students get these funds about every other year.
  • POSTERS: Poster-Making Guide by Steve Block (.pdf)
  • POSTERS: Poster-Making Guide by Colin Purrington (Overwhelmingly useful AND funny)
  • POSTERS: Free help with figure/poster design from the UW Design Help Desk
  • POSTERS: How to print and tile 8.5x11 sheets to tape together as a poster test from Adobe.
  • POSTERS: Here is how to contact UW printing centers for Hours and Locations, Prices, Directly submitting your work. Inquire about proofs, either as an 11x17, or as a 2" strip. As of 2017, Creative Communications poster printing is $60. CC's online form might say your poster will be done in a week, so call them to make sure you'll get your poster in time. Sometimes they do it in one day.
  • POSTERS: Printing large format posters in the SoACC (Rm 299 on the 2nd floor of the Art Bldg) costs $30-60 (as of 2017). M-Th 9am-10pm, Fri-Sun 10-6. For location, click HERE. For prices, click HERE. They can print posters that are 44"x62" or 44"x36". Leave 1/2" margins, so that's 43"x61" or 43"x35" max. Bring memory stick with poster in PDF with fonts embedded (or convert to outlines). Rotate once you're done to portrait instead of landscape. Powerpoint works worse. Expect lines if student art projects are due. They prefer files made by Adobe Illustrator - otherwise your file type may not talk to the printer and you may need help to format your file (e.g. Powerpoint). Ask if you should print a test. Expect to be humbled (rightly perhaps) that you are not using the most superior software that artists use. So far, JV fund transfer to a UW acct has been possible if arrange beforehand with staff (not student helper). You can also pay online with Visa, MasterCard, and Discover credit cards (click HERE.) If your printed poster needs to have excess paper trimmed, there is a large-format cutter in room 023 in the Art Bldg (do it yourself). Bring a pencil to mark trimming lines or include cutting guide lines in the printed poster design.
  • FIGURES: UW's color palette
  • FIGURES: Color schemes readable by colorblind people (Click on "colorblind safe")
  • FIGURES: Color palettes readable by colorblind viewers (RGB and HTML color codes)
  • FIGURES: ASBMB on figures for colorblind viewers
  • FIGURES: Free help with figure design from the UW Design Help Desk
  • FIGURES: Data visualization video by John Rauser
  • FIGURES: Guide to making good graphs (Novartis Graphics Principles Cheat Sheet)
  • TALKS: Free feedback from the UW Public Speaking Center
  • TALKS: Free UW public speaking course through Coursera
  • TALKS: UW Drama250 on "Presence: Voice, Speech, and Persona for Public Professionals" (Summer 2017 course)
  • TALKS: YouTube videos on eye contact, posture, pacing, fluency, gestures, volume, and tone.
  • TALKS: Advice on giving talks by (1) Bertil Hille (.doc), and (2) Edward De Robertis (.docx).
  • TALKS: Fake, but pretty, movie of membrane rafts. (See 0:55 and 7:00.)

How to Write & Publish a Paper or Dissertation / How to Search for & Reference Literature

How to Maintain Lab Safety

How to Get Lab Ethics Training

Mass Transit to/from Our Lab

  • From Seatac aiport, take light rail to the University of Washington Station. (Buy a ticket before boarding.)
  • From Downtown, enter the "bus tunnel", for example at Westlake Station, and board a either the light rail or a northbound UW express bus (#71/72/73, or the #74 if it says "Univ Express"). Bus fare is $2.75 (as of 2016). Sit near the driver if you need to ask directions or questions. Your closest stop to the university will probably be your 2nd opportunity to depart is after crossing a freeway bridge over water. Ask the driver.
  • From anywhere else, use the King County Metro Trip Planner by entering "University of Washington" into the "To" or "From" field.
  • Walk to Bagley Hall, found on this map, next to Drumheller Fountain.
  • One Bus Away coordinates for Bagley Hall.

How to Get Yourself a Fellowship, Job, Grant, Plan, Mentor, Career

  • See the Keller Lab Mentoring Site Here.

How to Reduce Stress

Dept. of Chemistry 351700, Univ. of Washington, Seattle, WA, 98195-1700
Back to the Keller Group Page.