Keller Lab Links

last edited (haphazardly) 2016
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Reserved for Keller Lab:

How to Perform Biophysical Techniques & Record Data

How to Attend Group Meetings

  • Location: CHB239 Tuesdays 2:00-3:30pm
  • How to propose to change the group meeting time/place:
    1) Ask a secretary if a conference room is available at your time (in order of preference: CHB239, 339, 439, BAG319)
    2) Set up a 5-day chart at when2meet.com with the times that you and the rooms are available.
    3) E-mail amphiphiliphiles - ask them to enter their schedules. Send reminders. Verify that you can still book the room.
    4) Inform everyone of the change. Send reminders before the meeting to help old dogs learn new tricks.

How and Where to Buy/Build Stuff & Learn about Lipids

Microscopes

  • Nikon's Microscopy U
  • TECHNIQUE: At the end of the travel on the fine focus knob? Back up and use coarse focus, then fine again. New lab members commonly ruin the fine focus by overtwisting. For example, if the lubrication inside is too old, then the fine focus will act as if it is fused with the coarse focus.
  • TECHNIQUE: Micromanager, a free ImageJ plug-in to control microscope cameras
  • TECHNIQUE: Many of our lenses have correction collars. Adjusting them properly for the cover slip you use makes a big difference.
  • TECHNIQUE:Is the lens stuck? Use a rubber strap wrench, not a metal wrench.
  • BUY: Lubricate microscopes with "Nyoil II" to lubricate microscopes
  • BUY: "Packing oil" to lubricate microscope adjusting screws, because it stays in place.
  • BUY: "Sparkle" glass cleaner to clean lenses and objectives (any department store), or "Formula MD" lens cleaner. Ethanol/isopropanol may eventually dissolve the glue holding the lens in place.
  • BUY: Photodiode light sources: Jan Strelow recommends Chris Baumann at 89 North or perhaps AMG (advanced microscopy group)
  • BUY: Filter cubes. Jan Strelow recommends Chroma, inform that have older metal cubes in which filters have problems and want new hard coats.

User Facilities and Makerspaces

  • UW Makerspace (appears to be free except for consumables)
  • Metrix Maker Space on Capitol Hill - electronics, laser cutter, 3D printer, sewing machine, classes... and coffee! Membership and walk-ins.
  • UW Analyltical Biopharmacy Core with ultracentrifuge, DSC, ITC, SPR, viscometer, etc. Apply for matching funds up to $5K/lab.

Statistics, Computing, and Programs

Other Links for Keeping the Keller Lab Functioning

Conferences / Travel / Posters / Talks

  • MEMBERSHIP: APS (specify DBIO, Division of Biological Sciences)
  • MEMBERSHIP: Biophysical Society (specify the MSAS subgroup)
  • CONFERENCES: Worldwide Biophysics Conferences, and also through the EBSA, HERE
  • CONFERENCES - BIOPHYSICAL SOCIETY: [1] Become a member or renew. To find your member #, search e-mail for "membership receipt". Choose student membership. Upload copy of student ID with SLK's signature. [2] From main page, click "submit an abstract" and log in. [3] Select "poster only" if competing in SRAA competition, which you should if a student. Typical topic #1:"3A: Membrane physical chemistry", #2 "3E: Membrane structure". [4] Select "student member combo fee" and pay with credit card. At Chem Dept site, print reimbursement sheet, attach receipt, and submit to BAG109P. Ariba will e-mail, requiring verification of reimbursement. [5] Apply to SRAA competition using member # and control invoice # (e.g. "14-A-___-BPS") from BPS e-mail titled "Payment Receipt". You will receive confirmation e-mail titled "BPS SRAA Receipt".
  • REIMBURSEMENT: Form for pre-approval of travel for Faculty and Students/Postdocs/Staff
  • REIMBURSEMENT: If you have passed your 3rd Year exam, request a travel award from your dept. If you receive one from the Chem Dept, forward our group's administrative staff person (currently Jon Geraghty geraghti@uw.edu) the e-mail that announced your award so that the travel funds can be used to reimburse you.
  • POSTERS: Poster-Making Guide by Steve Block (.pdf)
  • Poster-Making Guide by Colin Purrington (Overwhelmingly useful AND funny)
  • POSTERS: Free help with figure/poster design from the UW Design Help Desk
  • POSTERS: How to print and tile 8.5x11 sheets to tape together as a poster test from Adobe.
  • POSTERS: Here is how to contact UW printing centers for Hours and Locations, Prices, Directly submitting your work. Inquire about proofs, either as an 11x17, or as a 2" strip.
  • POSTERS: Printing large format posters on the 2nd floor of the Art Building costs $40 (as of 2011). M-Th 9am-10pm, Fri-Sun 10-6. Their printer is 44"x62", but leave 1/2" margins, so that's 43"x61" maximum. Bring memory stick with poster in PDF with fonts embedded (or convert to outlines). Rotate once you're done to portrait instead of landscape. Powerpoint works worse. Long lines if student art projects are due. They prefer files made by Adobe Illustrator - otherwise your file type may not talk to the printer and you may need to help you format your file (e.g. Powerpoint). Ask if you should print a test. Expect to be humbled (rightly perhaps) that you are not using the most superior software that real artists use. The main method of payment is via a card that you have to buy (beforehand? only on weekdays?). JV fund transfer may be possible if arrange beforehand. No web presence/info since they are already at capacity.
  • FIGURES: UW's color palette
  • FIGURES: Color schemes readable by colorblind people (Click on "colorblind safe")
  • FIGURES: Color palettes readable by colorblind viewers (RGB and HTML color codes)
  • FIGURES: Free help with figure design from the UW Design Help Desk
  • TALKS: Free help from the UW Public Speaking Center
  • TALKS: Advice on giving talks by (1) Bertil Hille (.doc), and (2) Edward De Robertis (.docx).
  • TALKS: Fake, but pretty, movie of membrane rafts. (See 0:55 and 7:00.)

How to Write & Publish a Paper or Dissertation / How to Search for & Reference Literature

How to Maintain Lab Safety

How to Get Lab Ethics Training

Mass Transit to/from Our Lab

  • From Seatac aiport, take light rail to the University of Washington Station. (Buy a ticket before boarding.)
  • From Downtown, enter the "bus tunnel", for example at Westlake Station, and board a either the light rail or a northbound UW express bus (#71/72/73, or the #74 if it says "Univ Express"). Bus fare is $2.75 (as of 2016). Sit near the driver if you need to ask directions or questions. Your closest stop to the university will probably be your 2nd opportunity to depart is after crossing a freeway bridge over water. Ask the driver.
  • From anywhere else, use the King County Metro Trip Planner by entering "University of Washington" into the "To" or "From" field.
  • Walk to Bagley Hall, found on this map, next to Drumheller Fountain.
  • One Bus Away coordinates for Bagley Hall.

How to Get Yourself a Fellowship, Job, Grant, Plan, Mentor, Career

  • See the Keller Lab Mentoring Site Here.

How We (Attempt to) Stay Sane


Dept. of Chemistry 351700, Univ. of Washington, Seattle, WA, 98195-1700
Back to the Keller Group Page.