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Tutorial#13: The use of KAP and VOL parameters
How to set up DSB formation and repair models

KAP, the peak cell density and VOL, the effective volume of the cell culture or the tissue region of interest determines the bounding conditions of cell multiplication.

 
Example 13.1   Time variation of the cell count after a multi-fraction exposure for different choices of the peak cell
                          density (rmrsim6.inp -> rmrsim6.out,  rmrsim7.inp -> rmrsim7.out).    
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Cell count  after multi-fraction dose exposure for different values
of the peak cell density, KAP.
 
Comments:
  • The cell culture volume is chosen as 1 cm3 so that the peak cell density (cells/cm3) directly represents the number of cells at a given time.
  • The number of cells sharply decreases during the time of irradiation.
  • The multiplication of cells plateaus to the specified peak cell density, KAP.
 
Suggested Problems
  1. Investigate the effect of the parameter VOL for a chosen value of KAP (you are expected to determine the plots that may be needed for the purpose).
  2. Suppose, the calculation shown in Example 13.1 for KAP = 140 denotes a cell culture that contains 100 cells at the beginning of irradiation. If 99% presence of the maximum cell population denotes confluence, how long does it take for this cell population to reach confluence?    
  
Tip: Experiment by changing the various model parameters and physically interpreting your results.
 
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Last updated: 10 June, 2011
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