UW
VMBHRC

Hume Lab

Virginia Merrill Bloedel Hearing Research Center

Department of Otolaryngology-Head and Neck Surgery

Protocols

 

General Molecular Biology

Midipreps Qiagen RT PCR In situ probe preparation
Minipreps Qiagen Colony PCR RNA preparation
Maxipreps Qiagen Sequencing Preparation  

Cell Culture

Mouse cochlear explant cultures Counting cells  
Spiral ganglion cultures Media List  
Collagen gel explant cultures Lipofection for growth factor pellets  

Viral Preparation

Adenovirus Adeno-Associated Virus Purification
Ad 293 Transfection for primary Adeno 293 FT Transfection CaPO4  
Ad 293 Adeno amplification 293 FT Transfection Lipofectamine 2000  

Immunohistochemistry

Slide ICC Collagen gel explant ICC  
Whole mount embryo ICC In situ hybridization (except probe prep)  
Slide explant ICC    

Map Links