robertslab.info - News and Updates

Marine science mentoring over the summer at FHL

Feature on UW SAFS Website

Uncovering Population-specific Resilience to Ocean Acidification in the Olympia Oyster

A Deep Dive into Our Latest Publication

The Double-Edged Sword of Triploidy in Pacific Oysters

New Research Highlights Increased Vulnerability to Climate Stressors

qPCR Analysis - C.gigas Matt George Poly:IC Diploids

Ran a “quick and dirty” qPCR analysis for the qPCR’s I’d previously run:

Transcript Identification and Alignments - C.virginica RNAseq with NCBI Genome GCF_002022765.2 Using Hisat2 and Stringtie on Mox Again

In the process of generating expression matrices for CEABIGR (GitHub repo), and in turn distance matrices for these samples, I realized a couple of things:

qPCR - C.gigas PolyIC Diploid MgCl2

Using diploid MgCl₂ control cDNA from 20230816, performed qPCR on the following primer sets selected by Steven (GitHub Issue), along with Cg_Actin as…

Reverse Transcription - C.gigas PolyIC Diploid MgCl2

After quantifying diploid MgCl₂ (Notebook) earlier today and proceeded to make cDNA. Reverse transcription was performed using oligo dT primers using M-MLV RT (Promega), per the manufacturer’s…

RNA Quantification - C.gigas PolyIC Diploid MgCl2

Steven asked me to generate cDNA (GitHub Issue) from Crassostrea gigas (Pacific oyster) RNA, as part of Matt George’s polyIC project. I had previously quantified RNA from…

Genetics: Does it really matter?

Here I will describe a recent…