The Lingappa Lab - Department of Global Health, University of Washington

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Jonathan Reed | Motoko Tanaka

Jon Reed

Bio:

Jonathan Reed is a graduate student in the Ph.D. in Molecular and Cellular Biology (MCB) and M.S. in Epidemiology joint program. As an undergraduate at Oregon State University, he majored in Life Sciences/Botany and studied suppressors of RNA silencing encoded by plant viruses in the laboratory of Dr. Valerian Dolja. In 2004, he entered the University of Washington (UW) MCB PhD program, and in 2005-2006, Jon completed his coursework for his M.S. in Epidemiology at UW. As an undergraduate, Jon received numerous awards, including two HHMI summer undergraduate research fellowships and the outstanding senior award at Oregon State University. In 2006, Jon received a 3-year NSF graduate research fellowship.

Research:

As a rotation student, Jon identifiied the HIV capsid assembly intermediate that appears to direct the nascent viral capsid from the cytosol to membranes. For his PhD thesis, Jon is studying the other cellular factors, besides ABCE1, that are present in HIV capsid assembly intermediates.

Publications:

Klein KC, Reed JC, Tanaka M, Nguyen V, Giri S, and Lingappa JR. HIV Gag-Leucine zipper chimeras form ABCE1-containing intermediates and RNAse-resistant immature capsids similar to those formed by wild-type HIV-1 Gag. Journal of Virology, 2011 Jul;85(14):7419-35. [Download]
Klein KC, Reed JC, and Lingappa JR. Intracellular destinies: degradation, targeting, assembly, and endocytosis of HIV Gag. AIDS Reviews. 2007 Jul-Sep;9(3):150-61. [Abstract]
Dooher, Julia E., Schneider, Bobbie L., Reed, Jonathan C. & Lingappa, Jaisri R. (2006) Host ABCE1 is at plasma membrane HIV assembly sites and its dissociation from Gag is linked to subsequent events of virus production. Traffic. 2007 Mar; 8(3):195-211[Abstract]
TChiba M, Reed JC, Prokhnevsky AI, Chapman EJ, Mawassi M, Koonin EV, Carrington JC, Dolja VV. Diverse suppressors of RNA silencing enhance agroinfection by a viral replicon. Virology. 2006 Mar 1;346(1):7-14.
Tzanetakis IE, Reed J, Martin RR. Nucleotide sequence, genome organization and phylogenetic analysis of Strawberry pallidosis associated virus, a new member of the genus Crinivirus. Arch Virol. 2005 Feb;150(2):273-86.
Reed JC, Kasschau KD, Prokhnevsky AI, Gopinath K, Pogue GP, Carrington JC, Dolja VV. Suppressor of RNA silencing encoded by Beet yellows virus. Virology. 2003 Feb 15;306(2):203-9.

Motoko Tanaka

Bio:

Motoko Tanaka grew up in Awaji Island in Japan, which is famous for having the world's longest suspension bridge, the fourth fastest currents in the world (in the Naruto whirlpools), and a 500-year-old form of traditional puppet theatre. She did her undergraduate studies at the University of Tokushima, where she majored in Biological science and Technology and worked in the laboratory of Professor Hitoshi Hori. In 2007, she entered the Kobe University PhD program, where she studied host genetic factors related to the susceptibility to Mycobacterium infection in the infection control laboratory of Dr. Masato Kawabata. In 2008, she moved to the laboratory of Dr. Yoshihiro Kawaoka, in the Division of Zoonosis, Department of Microbiology and Infectious Diseases, Graduate School of Medicine at Kobe University, where she studied the host adaptation mechanism of influenza virus. In April 2010, she joined the Lingappa laboratory as a visiting graduate student, through the University of Washington School of Medicine and Kobe University Graduate School of Medicine exchange program and the Kobe University Global COE Program Kobe University Global COE Program funded by the Ministry of Education, Culture, Sports, Science, and Technology of Japan. She is currently enrolled in the Visiting International Student Internship and Training Program (VISIT) at University of Washington.

Outside of lab, Motoko enjoys traveling to foreign countries, photography, and hiking.

Research in the Lingappa Lab:

In her first project in the Lingappa lab, Motoko worked with Kevin Klein and Jon Reed studying the role of the nucleocapsid domain in HIV capsid assembly using Gag-leucine zipper chimeras (Klein, Reed, Tanaka, et al., manuscript submitted). Currently, she is working on identifying the binding site for ABCE1 in HIV-1 Gag and understanding how HIV-1 capsid assembly is linked to budding.

Publications:

Klein KC, Reed JC, Tanaka M, Nguyen V, Giri S, and Lingappa JR. HIV Gag-Leucine zipper chimeras form ABCE1-containing intermediates and RNAse-resistant immature capsids similar to those formed by wild-type HIV-1 Gag. Journal of Virology, 2011 Jul;85(14):7419-35. [Download]
Toll-like receptor pre-stimulation protects mice against lethal infection with highly pathogenic influenza viruses. Shinya K, Okamura T, Sueta S, Kasai N, Tanaka M, Ginting TE, Makino A, Eisfeld AJ, Kawaoka Y. Virol J. 2011 Mar 4;8:97. [Abstract]
Design, synthesis, and radiosensitizing activities of sugar-hybrid hypoxic cell radiosensitizers. Nakae T, Uto Y, Tanaka M, Shibata H, Nakata E, Tominaga M, Maezawa H, Hashimoto T, Kirk KL, Nagasawa H, Hori H. Bioorg Med Chem. 2008 Jan 15;16(2):675-82. [Abstract]
NRAMP1/SLC11A1 gene polymorphisms and host susceptibility to Mycobacterium tuberculosis and M. leprae in South Sulawesi, Indonesia. Hatta M, Ratnawati, Tanaka M, Ito J, Shirakawa T, Kawabata M. Southeast Asian J Trop Med Public Health. 2010 Mar;41(2):386-94. [Abstract]