Mac’s April Goals

Posted in tumblelog

This has been reposted from our lab tumblelog

This month has started off with a flurry of emails (from: me, to: those more experienced), asking for advice.  This is a wonderful thing.  It is so awesome to get enthusiastic responses from colleagues who are willing to share their knowledge and pass on your questions to other experts in the field.  It makes me love science even when I don’t know what I’m doing.

The first thing I have been looking for expertise on is determining sex in early oyster gonad samples.  I am very confident that I can determine sex of a late stage oyster gonad using histology, but the early gonad is quite challenging as early male and female gonad tissue can be almost indistinguishable.  I have posted pictures of the slides and have been getting some great feedback and suggestions for helpful literature (e.g. Franco et al 2010 ).  After sex has been determined, I’ll be isolating DNA and looking to see how DNA methylation changes in response to synthetic estrogen exposure.

The other thing I have been soliciting advice on is the development of educational computer games.  So far, Jessica Rhode has sent me a great storify on science video games and I am finding really cool examples on UW’s Center for Game Science page.  My goal for this month is to develop more ideas for the ‘Oyster Game of Life’ and hopefully identify a student that would be interested in helping with this project.

A few other things going on this month include getting some DNA methylation data back from Nanostring (samples include oysters whose parents were exposed to a pesticide), and starting a new class, Studies on local adaptation: why, what, how and where? (FISH 510).

I will also need to figure out how to exact revenge for this..

via the Lab Tumblr: http://genefish.tumblr.com/post/47046926088