Sablefish Broodstock Development and Functional Genomics

Posted in Research


Research Summary: Sablefish (Anoplopoma fimbria, aka black cod) is an excellent species for commercial culture because of its high price and fast growth in culture.  NOAA is developing the resources and techniques for intensive aquaculture of this species including the establishment of broodstocks from the wild and the production of monosex and sterile stocks to enhance growth and mitigate escapement concerns, respectively.  Domesticated broodstocks will be an essential resource as the industry moves forward since it provides a known genetic background upon which further improvement in traits such as growth and disease resistance can be selected.  While there have been some expressed sequence tags (ESTs) released for this species, there are no genetic markers for sablefish that can be used to pedigree broodstocks and their offspring in the hatchery or genomic markers that can be used for selection of traits of interest.  NOAA is investigating the production of monsex or sterile stocks of sablefish by applying steroids such as 17a-methyltestosterone (17a-MT) at early life stages.  However, the effects of these treatments on growth and the expression of genes related to metabolic and growth pathways are unknown.  These effects are important for assessing the overall impact of steroids that are being used for reproductive manipulation. Thus, the goals of this work are to produce the molecular (genomic) tools and information required for NOAA to help establish sablefish broodstock for commercial aquaculture (i.e. genomic markers that can be used in selective breeding and to assess impacts of 17a-MT on growth and metabolism).

 

 

 

 

 

 

 

 

 

 

This research effort is in collaboration with

Dr. Frederick Goetz at the NOAA Manchester Research Station

 

This research effort is funded by: