HCV Capsid Assembly

The 100S HCV capsids from the cell-free system (CFS) are morphologically similar to authentic capsids. (A and B) Cell-free reactions programmed with C191 transcript were separated by velocity sedimentation, and fractions 6, 7, and 8 (containing 100S capsids) were pooled, dialyzed against phosphate-buffered saline, and subjected to negative-stain TEM. (C) Serum from an infected patient, treated with detergent to remove envelopes, was processed in parallel. Bars, 100 nm. From Klein, Polyak, and Lingappa, J. Virol. 78:957 (2004).

HBV Capsid Assembly | HCV Capsid Assembly | HIV-1 Capsid Assembly