PUBLICATIONS

Hamilton GE, Helgeson LA, Noland CL, Asbury CL, Dimitrova YN, Davis TN, "Reconstitution reveals two paths of force transmission through the kinetochore." (2020) eLIFE 9:e56582. doi:10.7554/eLife.56582. (pdf)

Miller MP, Evans RK, Zelter A, Geyer EA, MacCoss MJ, Rice LM, Davis TN, Asbury CL, Biggins S, "Kinetochore-associated Stu2 promotes chromosome biorientation in vivo." (2019) PLoS Genetics 15(10), e1008423. (pdf)

Koch LB, Opoku KN, Deng Y, Barber A, Littleton AJ, London N, Biggins S, & Asbury CL, "Autophosphorylation is sufficient to release Mps1 kinase from native kinetochores." (2019) Proc. Natl. Acad. Sci. USA 116(35), 17355–17360. (pdf)

Scarborough EA, Davis TN, Asbury CL, "Tight bending of the Ndc80 complex provides intrinsic regulation of its binding to microtubules." (2019) eLIFE 8:e44489. doi:10.7554/eLife.44489. (pdf)

Larson JD, Asbury CL, "Relax, kinetochores are exquisitely sensitive to tension" (2019) Developmental Cell 49(1):5-7. doi: 10.1016/j.devcel.2019.03.019. (pdf)
Comment on: Mukherjee et al., "A gradient in metaphase tension leads to a scaled cellular response in mitosis" Developmental Cell 49(1):63-76. (link)

Llauró A, Hayashi H, Bailey ME, Wilson A, Ludzia P, Asbury CL, Akiyoshi B, "The Kinetoplastid kinetochore protein KKT4 is an unconventional microtubule tip-coupling protein" (2018) Journal of Cell Biology 217(11):3886-3900. (pdf)

Jung SR, Deng Y, Kushmerick C, Asbury CL, Hille B, Koh DS, "Minimizing ATP depletion by oxygen scavengers for single-molecule fluorescence imaging in live cells" (2018) Proc. Natl. Acad. Sci. USA 115(25):E5706-E5715. (pdf)

Helgeson LA, Zelter A, Riffle M, MacCoss MJ, Asbury CL, Davis TN, "Human Ska complex and Ndc80 complex interact to form a load-bearing assembly that strengthens kinetochore-microtubule attachments" (2018) Proc. Natl. Acad. Sci. USA 115(11):2740-2745. (pdf)

Asbury CL, "Anaphase A: Disassembling microtubules move chromosomes toward spindle poles" (2017) Biology (Basel) 6(1) doi: 10.3390/biology6010015. (pdf)
Part of a special issue, "Mechanics of Mitotic Chromosome Segregation", edited by McIntosh JR and available free online (link)

Fong KK, Sarangapani KK, Yusko EC, Riffle M, Llauró A, Graczyk B, Davis TN, Asbury CL, "Direct measurement of the strength of microtubule attachment to yeast centrosomes" (2017) Molecular Biology of the Cell 28(14):1853-61. (pdf)

Kim JO, Zelter A, Umbreit NT, Bollozos A, Riffle M, Johnson R, MacCoss MJ, Asbury CL, Davis TN, "The Ndc80 complex bridges two Dam1 complex rings" (2017) eLIFE doi: 10.7554/eLife.21069. (pdf)

Driver JW, Geyer EA, Bailey ME, Rice LM, Asbury CL, "Direct measurement of conformational strain energy in protofilaments curling outward from disassembling microtubule tips" (2017) eLIFE doi: 10.7554/eLife.e28433. (pdf)

Jung SR, Seo JB, Deng Y, Asbury CL, Hille B, Koh DS, "Contributions of protein kinases and β-arrestin to termination of protease-activated receptor 2 signaling" (2016) Journal of General Physiology 147(3):255-271. (pdf)

Miller MP, Asbury CL, Biggins S, "A TOG protein confers tension sensitivity to kinetochore-microtubule attachments" (2016) Cell 165:1428-39. (pdf)
Highlighted in: Cheerambathur DK, Prevo B, and Desai A, "A TOGgle for tension at kinetochores" (2016) Cell 165:1316-8. (pdf)
Highlighted in: McCarthy M, "Tension-sensitive protein a key player in cell division" (2016) NewsBeat UW Health Sciences. (link)

Deng Y, Asbury CL, "Simultaneous Manipulation and Super-Resolution Fluorescence Imaging of Individual Kinetochores Coupled to Microtubule Tips" (2016) in Optical Tweezers: Methods and Protocols, edited by A Gennerich, Methods in Molecular Biology 1486:437-467. (pdf)

Kudalkar EM, Davis TN, Asbury CL, "Single-Molecule Total Internal Reflection Fluorescence Microscopy" (2016) Cold Spring Harbor Protocols doi: 10.1101/pdb.top077800. (pdf)

Asbury CL, "Data Analysis for Total Internal Reflection Fluorescence Microscopy" (2016) Cold Spring Harbor Protocols doi: 10.1101/pdb.prot085571. (pdf)

Kudalkar EM, Davis TN, Asbury CL, "Preparation of Reactions for Imaging with Total Internal Reflection Fluorescence Microscopy" (2016) Cold Spring Harbor Protocols doi: 10.1101/pdb.prot085563.(pdf)

Kudalkar EM, Deng Y, Davis TN, Asbury CL, "Coverslip Cleaning and Functionalization for Total Internal Reflection Fluorescence Microscopy." (2016) Cold Spring Harbor Protocols doi: 10.1101/pdb.prot085548. (pdf)

Kudalkar EM, Scarborough EA, Umbreit NT, Zelter A, Gestaut DR, Riffle M, Johnson RS, MacCoss MJ, Asbury CL, Davis TN, "Regulation of Ndc80-based microtubule attachments by the central kinetochore Mis12/MIND complex" (2015) Proc. Natl. Acad. Sci. USA 112(41):E5583-9. (pdf)

Sarangapani KK, Duro E, Deng Y, Alves FL, Ye Q, Opoku KN, Ceto S, Rappsilber J, Corbett KD, Biggins S, Marston AL, Asbury CL, "Sister kinetochores are mechanically fused during meiosis I in yeast" (2014) Science 346(6206):248-51. (pdf)
Highlighted in: Gray L, "Cells simply avoid chromosome confusion" (2014) NewsBeat UW Health Sciences. (link)
Highlighted in: O'Sullivan K, "Study could aid understanding of infertility" (2014) Business Scotland. (link)
Adèle interviewed on BBC Radio 4 - Today (beginning ~50 min into the program)

Yusko EC, Asbury CL, "Force is a signal that cells cannot ignore" (2014) Molecular Biology of the Cell 25:3717-25. (pdf)

Umbreit NT, Miller MP, Tien JF, Cattin-Ortola J, Gui L, Lee KK, Biggins S, Asbury CL, Davis TN, "Kinetochores require oligomerization of the Dam1 complex to maintain microtubule attachments against tension and promote biorientation" (2014) Nature Communications 5(4951)1-11. (pdf)

Tien JF, Umbreit NT, Zelter A, Riffle M, Hoopmann MR, Johnson RS, Fonslow BR, Yates JR, MacCoss MJ, Moritz RL, Asbury CL, Davis TN, "Kinetochore biorientation in Saccharomyces cerevisiae requires a tightly folded conformation of the Ndc80 complex" (2014) Genetics 198:1483-93. (pdf)
Highlighted in: Costanzo M, "A lot hinges on Ndc80" (2014) New & Noteworthy Research Spotlight, Saccharomyces Genome Database (link)

Sarangapani KK, Asbury CL, “Catch and release: How do kinetochores hook the right microtubules during mitosis?” (2014) Trends in Genetics 30(4):150-159. (pdf)
Featured on the cover of Trends in Genetics. (link)

Driver JW, Powers AF, Sarangapani KK, Biggins S, Asbury CL, “Measuring kinetochore-microtubule interaction in vitro” (2014) Methods in Enzymology 540:321-337.

Decarreau J, Driver J, Asbury CL, Wordeman L, "Rapid measurement of mitotic spindle orientation in cultured mammalian cells" (2014) Methods in Molecular Biology 1136:31-40. (pdf)

Tien JF, Fong KK, Umbreit NT, Payen C, Zelter A, Asbury CL, Dunham MJ, Davis TN, "Coupling unbiased mutagenesis to high-throughput DNA sequencing uncovers functional domains in the Ndc80 kinetochore protein of Saccharomyces cerevisiae" (2013) Genetics 195(1):159-170. (pdf)

Sarangapani KK, Akiyoshi B, Duggan NM, Biggins S, Asbury CL, "Phosphoregulation promotes release of kinetochores from dynamic microtubules via multiple mechanisms" (2013) Proc. Natl. Acad. Sci. USA 110(18):7282-7. (pdf)

Umbreit NT, Gestaut DR, Tien JF, Vollmar BS, Gonen T, Asbury CL, Davis TN, "The Ndc80 kinetochore complex directly modulates microtubule dynamics" (2012) Proc. Natl. Acad. Sci. USA 109(40):16113-8. (pdf)
Discussed in: Nannas NJ, Murray AW, "Complications dawn for kinetochore regulation by Aurora" (2012) Proc. Natl. Acad. Sci. USA 109(40):15972-3. (pdf)

Stumpff J, Wagenbach M, Franck A, Asbury CL, Wordeman L, "Kif18A and chromokinesins confine centromere movements via microtubule growth suppression and spatial control of kinetochore tension" (2012) Developmental Cell 22(5):1017-29. (pdf)
Jason's kymographs featured on the cover of Developmental Cell. (link)

Stumpff J, Du Y, English CA, Maliga Z, Wagenbach M, Asbury CL, Wordeman L, Ohi R, "A tethering mechanism controls the processivity and kinetochore-microtubule plus-end enrichment of the kinesin-8 Kif18A" (2011) Molecular Cell 43(5):764-75.(pdf)

Asbury CL, Tien JF, Davis TN, "Kinetochores' gripping feat: biased diffusion or conformational wave?" (2011) Trends in Cell Biology 21(1):38-46. (pdf)

Akiyoshi B, Sarangapani KK, Powers AF, Nelson CR, Reichow SL, Arellano-Santoyo H, Gonen T, Ranish JA, Asbury CL, Biggins S, "Tension directly stabilizes reconstituted kinetochore-microtubule attachments" (2010) Nature, 468(7323):576-9. (pdf)
Discussed in: Shimamoto Y, "Cellular seat belts" (2010) Nature, 468(7323):518-9. (pdf)
Highlighted in: Gray L, "Finger-trap tension stabilizes cells' chromosome separating machinery" (2010) UW news and information. (link)

Tien JF, Umbreit NT, Gestaut DR, Franck AD, Cooper J, Wordeman L, Gonen T, Asbury CL, Davis TN, "Cooperation of the Dam1 and Ndc80 kinetochore complexes enhances microtubule coupling and is regulated by aurora B" (2010) Journal of Cell Biology 189(4):713-23. (pdf)
Discussed in: Short B, "Kinetochores hang on for the ride" (2010) Journal of Cell Biology 189(4):615. (pdf)

Wargacki MM, Tay JC, Muller EG, Asbury CL, Davis TN, "Kip3, the yeast kinesin-8, is required for clustering of kinetochores at metaphase" (2010) Cell Cycle 9(13):2581-8. (pdf)
Discussed in: Rizk R, Gupta ML, "Kip3 clusters kinetochores" (2010) Cell Cycle 9(13):2496. (pdf)
Discussed in: Ohi R, "Kip3-ing kinetochores clustered" (2010) Cell Cycle 9(13):2497. (pdf)

Gestaut DR, Cooper J, Asbury CL, Davis TN, Wordeman L, "Reconstitution and functional analysis of kinetochore subcomplexes" (2010) Methods in Cell Biology 95:641-56. (pdf)

Franck AD, Powers AF, Gestaut DR, Davis TN, Asbury CL, "Direct physical study of kinetochore-microtubule interactions by reconstitution and interrogation with an optical force clamp" (2010) Methods 51(2):242-50. (pdf)

Powers AF, Franck AD, Gestaut DR, Cooper J, Gracyzk B, Wei RR, Wordeman L, Davis TN, Asbury CL, "The Ndc80 kinetochore complex forms load-bearing attachments to dynamic microtubules via biased diffusion" (2009) Cell 136:865-875. (pdf)
Discussed in: Joglekar AP and DeLuca JG, "Chromosome segregation: Ndc80 can carry the load" (2009) Current Biology R404-R407. (pdf)
Highlighted in: Gray L, "Single molecule tracking helps reveal mechanism of chromosome separation in dividing cells" (2009) UW news and information. (link)
Online interview with Chip: posted at Cell PaperClips (podcast)

Cooper JR, Wagenbach M, Asbury CL, Wordeman L, "Catalysis of the microtubule on-rate is the major parameter regulating the depolymerase activity of MCAK" (2009) Nature Structural & Molecular Biology 17(1):77-82. (pdf)

Fehr A, Gutiérrez-Medina B, Asbury CL, Block SM, "On the origin of kinesin limping" (2009) Biophysical Journal 97(6):1663-1670. (pdf)

Asbury CL and Davis TN, "Insights into the kinetochore" (2008) Structure 16:834-836. (pdf)

Asbury CL, “XMAP215: a tip tracker that really moves” (2008) Cell 132:19-20. (pdf)

Gestaut DR, Graczyk B, Cooper J, Widlund PO, Zelter A, Wordeman L, Asbury CL, and Davis TN, "Phosphoregulation and depolymerization-driven movement of the Dam1 complex do not require ring formation" (2008) Nature Cell Biology 10(4):407-414. (pdf)
Discussed in: Gardner MK and Odde DJ, "Dam1 complexes go it alone on disassembling microtubules" (2008) Nature Cell Biology 10(4):379-381. (pdf)

Fehr A, Asbury CL, Block SM, "Kinesin steps do not alternate in size" (2008) Biophysical Journal 94(3):L20-22. (pdf)

Stumpff J, Von Dassow G, Wagenbach M, Asbury CL, Wordeman L, “The Kinesin-8 motor Kif18A suppresses kinetochore movements to control mitotic chromosome alignment” (2008) Developmental Cell 14:252-262. (pdf)
Discussed in: Gardner MK, Odde DJ, and Bloom K, "Kinesin-8 molecular motors: putting the brakes on chromosome oscillations" (2008) Trends in Cell Biology 18(7):307-310. (pdf)

Stumpff J, Asbury CL, “Chromosome bi-orientation: euclidian euploidy” (2007) Current Biology 18(2):R81-83. (pdf)

Franck AD, Powers AF, Gestaut DR, Gonen T, Davis TN, and Asbury CL, “Tension applied through the Dam1 complex promotes microtubule elongation providing a direct mechanism for length control in mitosis” (2007) Nature Cell Biology 9:832-837. (pdf)
Discussed in: Gardner MK, Hunt AJ, Goodson HV, and Odde DJ, "Microtubule assembly dynamics: new insights at the nanoscale" (2008) Current Opinion in Cell Biology 20:64-70. (pdf)

Asbury CL, Gestaut DR, Powers AF, Franck AD, and Davis TN, “The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement” (2006) Proc. Natl. Acad. Sci. USA 103(26):9873-9878. (pdf)

Asbury CL “Kinesin: World’s Tiniest Biped” (2005) Current Opinion in Cell Biology 17:89-97. (pdf)

Asbury CL, and Block SM, “Motor proteins” (2005) in Encyclopedia of Molecular Cell Biology and Molecular Medicine, RA Meyers, Ed., Wiley-VCH, Weinheim, Germany, pgs. 575-600. (pdf)

Asbury CL, Fehr AN, and Block SM, “Kinesin Moves by an Asymmetric Hand-over-hand Mechanism” (2003) Science, 302:2130-2134. (pdf)
Discussed in: Perkel JM "Investigating molecular motors step by step" (2004) The Scientist 18:19-23. (pdf)
Discussed in: "Breakthrough of the year: the runners-up" (2003) Science 302:2039-2045. (pdf)

Block SM, Asbury CL, Shaevitz JW, and Lang MJ, “Probing the Kinesin Reaction Cycle with a Two-Dimensional Optical Force Clamp” (2003) Proc. Natl. Acad. Sci. USA 100(5):2351-2356. (pdf)

Lang MJ, Asbury CL, Shaevitz JW, and Block SM, “An Automated Two-Dimensional Optical Force Clamp for Single Molecule Studies” (2002) Biophysical Journal 83:491-501. (pdf)

Uy JL, Asbury CL, Petersen TW, and van den Engh G, “The polarization of fluorescence of DNA stains depends on the incorporation density of the dye molecules” (2004) Cytometry 61:18-25. (pdf)

Asbury CL, Diercks AH, and van den Engh G, “Trapping of DNA by Dielectrophoresis” (2002) Electrophoresis 23:2658-2666. (pdf)

Asbury CL, Uy JL, and van den Engh G, “Polarization of Scatter and Fluorescence Signals in Flow Cytometry” (2000) Cytometry 40:88-101. (pdf)
Discussed in: Shapiro HM, "Everything you never wanted to know about polarization -- but were afraid you might find out" (2000) Cytometry 40:87. (pdf)

Asbury CL, and van den Engh G, “Trapping of DNA in Nonuniform Oscillating Electric Fields” (1998) Biophysical Journal 74:1024-1030. (pdf)

Asbury CL, Esposito R, Farmer C, and van den Engh G, “Fluorescence Spectra of DNA Dyes Measured in a Flow Cytometer” (1996) Cytometry 24:234-242.

Peattie RA, Asbury CL, Bluth EI, and Ruberti JW, “Steady Flow in Models of Abdominal Aortic Aneurysms. Part I: Investigation of the Velocity Patterns” (1996) Journal of Ultrasound in Medicine 15:679-688.

Peattie RA, Asbury CL, Bluth EI, and Ruberti JW, “Steady Flow in Models of Abdominal Aortic Aneurysms. Part II: Wall Stresses and their Implication for In Vivo Thrombosis and Rupture” (1996) Journal of Ultrasound in Medicine 15:689-696

Asbury CL, Ruberti JW, Bluth EI, and Peattie RA, “Experimental Investigation of Steady Flow in Rigid Models of Abdominal Aortic Aneurysms” (1995) Annals of Biomedical Engineering 23:29-39.