Model-building and refinement into cryoEM density

Our cryoEM model-building and refinement tools are included as part of the Rosetta software package.


The tutorial is intended to introduce users to several different ways Rosetta may be used to solve various structure determination tasks given 3-5Å cryoEM density data.

These tutorials are based on a tutorial given at the hybrid methods conference, in Lake Tahoe, March 2015.

Last updated, April 2017.

The complete tutorial: [instructions (pdf)] [all files (tgz)]

Download individual parts:

  • Part 1: Introduction to Rosetta for cryoEM  [files (tgz)]
  • Part 2: Fragment-based structure refinement [files (tgz)]
  • Part 3: Model rebuilding using RosettaCM [files (tgz)]
  • Part 4: De novo structure determination [files (tgz)]

If you get XML parsing errors while running the above, try an older version of the tutorial [all files.2015 (tgz)].

Slides from the Jülich “Hands-on” Workshop on Computational Biophysics, August 2016

  • Lecture 1 – Intro to Rosetta for cryoEM modelling [pdf]
  • Lecture 2 – Advanced Rosetta topics [pdf]