=============================== MCDS Version 2.01 19-JAN-2006 =============================== 20.379 running time (min) 1141746432 seed 100000 number of cells RADIATION TYPE: 1H 3.2000E+00 Kinetic energy (MeV) >= 1.0487E-01 MeV 9.3829E+02 Rest mass energy (MeV) 8.2379E-02 Speed (beta=v/c) 1.4735E+02 (Zeff/beta)^2 <= 3200 DAMAGE FORMATION AND CLUSTERING: 105246 segment length, nseg (bp per cell per Gy) 1300 number of strand breaks, sigSb (per cell per Gy) 3900 number of base damages, sigBd (per cell per Gy) 3.0 base damage to strand break ratio, f 9 minimum distance between clusters, Nmin (bp) 10 maximum distance between two Sb to compose a DSB, Ndsb (bp) 0.0 fraction Bl/Bd DMSO SIMULATION: 5.0000E-01 fraction of non-scavengeable DNA damage 5.0000E-01 concentration at half-level (mol dm^-3) 0.0000E+00 DMSO concentration (mol dm^-3) % DAMAGE YIELD (See Nikjoo et al. 1997 Int.J.Radiat.Biol. 71, 467-483; Nikjoo et al. 2001 Radiat.Res. 156, 577-583) =================================================================================================================== 65.381 BD - one or more Bd (no Sb) 30.184 SSB - Sb (no auxilary damage) 2.013 SSB+ - two Sb on the same strand 0.346 2SSB - two or more Sb on opposite strands separated by at least 10 bp 1.680 DSB - two Sb on opposite strands with a separation <= 10 bp 0.336 DSB+ - DSB accompanied by one (or more) additional Sb within 10 bp separation 0.060 DSB++ - more than one DSB whether within the 10 bp separation or further apart 7.250 SSBc - fraction of complex damage (SSB+ and 2SSB) among SSBs 51.629 SSBcb - fraction of complex damage (SSB+ and 2SSB) among SSBs; base damage included 19.092 DSBc - fraction of complex damage (DSB+ and DSB++) among DSBs 63.715 DSBcb - fraction of complex damage (DSB+ and DSB++) among DSBs; base damage included =================================================================================================================== NUMBER OF CLUSTERS (per cell per Gy) =================================================================================================================== Number of --------- DSB ---------- --------- SSB ---------- -------- OTHER --------- ----- ALL CLUSTERS ----- lesions Average SEM Average SEM Average SEM Average SEM =================================================================================================================== 1 --- --- 5.15351E+02 5.74798E-02 1.54564E+03 1.03671E-01 2.06099E+03 1.22782E-01 2 2.46726E+01 1.52561E-02 3.09629E+02 4.92247E-02 4.29792E+02 5.63924E-02 7.64094E+02 7.47788E-02 3 2.06986E+01 1.39216E-02 1.42770E+02 3.51509E-02 1.19361E+02 3.15657E-02 2.82830E+02 4.67653E-02 4 1.19286E+01 1.06771E-02 5.96559E+01 2.32229E-02 3.31103E+01 1.73775E-02 1.04695E+02 2.96518E-02 5 5.91877E+00 7.59536E-03 2.36542E+01 1.49340E-02 9.18081E+00 9.39762E-03 3.87537E+01 1.87701E-02 6 2.70878E+00 5.18268E-03 9.07923E+00 9.36485E-03 2.54503E+00 5.01922E-03 1.43330E+01 1.16470E-02 7 1.19112E+00 3.43873E-03 3.40093E+00 5.78028E-03 7.05010E-01 2.63996E-03 5.29706E+00 7.19596E-03 8 5.06570E-01 2.24482E-03 1.26057E+00 3.53973E-03 1.95480E-01 1.39323E-03 1.96262E+00 4.39616E-03 9 2.11590E-01 1.45397E-03 4.58570E-01 2.14287E-03 5.48700E-02 7.40944E-04 7.25030E-01 2.69137E-03 10 8.69200E-02 9.31374E-04 1.67390E-01 1.29604E-03 1.43400E-02 3.78081E-04 2.68650E-01 1.63939E-03 11 3.51300E-02 5.92252E-04 5.93200E-02 7.69816E-04 4.04000E-03 2.00592E-04 9.84900E-02 9.91518E-04 12 1.38900E-02 3.71446E-04 2.13300E-02 4.63845E-04 9.90000E-04 9.94500E-05 3.62100E-02 6.02488E-04 13 5.04000E-03 2.24380E-04 7.42000E-03 2.72489E-04 3.00000E-04 5.47643E-05 1.27600E-02 3.57454E-04 14 2.23000E-03 1.49166E-04 2.57000E-03 1.60107E-04 5.00000E-05 2.23602E-05 4.85000E-03 2.20148E-04 15 9.30000E-04 9.63921E-05 9.50000E-04 9.74221E-05 4.00000E-05 1.99997E-05 1.92000E-03 1.38432E-04 16 2.90000E-04 5.38441E-05 3.40000E-04 5.82999E-05 1.00000E-05 1.00000E-05 6.40000E-04 7.99748E-05 17 1.20000E-04 3.46391E-05 6.00000E-05 2.44943E-05 --- --- 1.80000E-04 4.24228E-05 18 8.00000E-05 2.82833E-05 2.00000E-05 1.41421E-05 --- --- 1.00000E-04 3.16214E-05 19 1.00000E-05 1.00000E-05 --- --- --- --- 1.00000E-05 1.00000E-05 20 --- --- --- --- --- --- --- --- 21 --- --- --- --- --- --- --- --- 22 --- --- --- --- --- --- --- --- 23 --- --- --- --- --- --- --- --- 24 --- --- --- --- --- --- --- --- 25 --- --- --- --- --- --- --- --- 26 --- --- --- --- --- --- --- --- 27 --- --- --- --- --- --- --- --- 28 --- --- --- --- --- --- --- --- 29 --- --- --- --- --- --- --- --- 30 --- --- --- --- --- --- --- --- 31 --- --- --- --- --- --- --- --- 32 --- --- --- --- --- --- --- --- 33 --- --- --- --- --- --- --- --- 34 --- --- --- --- --- --- --- --- 35 --- --- --- --- --- --- --- --- 36 --- --- --- --- --- --- --- --- 37 --- --- --- --- --- --- --- --- 38 --- --- --- --- --- --- --- --- 39 --- --- --- --- --- --- --- --- 40 --- --- --- --- --- --- --- --- 41 --- --- --- --- --- --- --- --- 42 --- --- --- --- --- --- --- --- 43 --- --- --- --- --- --- --- --- 44 --- --- --- --- --- --- --- --- 45 --- --- --- --- --- --- --- --- 46 --- --- --- --- --- --- --- --- 47 --- --- --- --- --- --- --- --- 48 --- --- --- --- --- --- --- --- 49 --- --- --- --- --- --- --- --- 50 --- --- --- --- --- --- --- --- Total 6.79812E+01 2.32894E-02 1.06552E+03 5.25847E-02 2.14060E+03 8.43402E-02 3.27410E+03 8.77975E-02 ------------------------------------------------------------------------------------------------------------------- NOTES: The damage yields are for an average human cell (genome length = 6E+09 base pairs). The category OTHER includes all classes of single and clustered damage sites that do not contain strand breaks (Sb). SEM - standard error of the mean. CLUSTER COMPOSITION (% Sb) =================================================================================================================== Number of --------- DSB ---------- --------- SSB ---------- -------- OTHER --------- ----- ALL CLUSTERS ----- lesions Average SEM Average SEM Average SEM Average SEM =================================================================================================================== 1 --- --- 1.00000E+02 0.00000E+00 0.00000E+00 0.00000E+00 2.50050E+01 2.34722E-03 2 1.00000E+02 0.00000E+00 5.37230E+01 2.33904E-03 0.00000E+00 0.00000E+00 2.49980E+01 2.94052E-03 3 7.21019E+01 8.75071E-03 3.90525E+01 3.45124E-03 0.00000E+00 0.00000E+00 2.49886E+01 4.29923E-03 4 5.87942E+01 1.29449E-02 3.21219E+01 5.18177E-03 0.00000E+00 0.00000E+00 2.49996E+01 6.41130E-03 5 5.11240E+01 1.94679E-02 2.81817E+01 7.96371E-03 0.00000E+00 0.00000E+00 2.50056E+01 9.73525E-03 6 4.61493E+01 2.91812E-02 2.57128E+01 1.29063E-02 0.00000E+00 0.00000E+00 2.50069E+01 1.52143E-02 7 4.26678E+01 4.04482E-02 2.40311E+01 2.21167E-02 0.00000E+00 0.00000E+00 2.50210E+01 2.52920E-02 8 3.99575E+01 5.65132E-02 2.28455E+01 3.36464E-02 0.00000E+00 0.00000E+00 2.49758E+01 3.96883E-02 9 3.80389E+01 8.22628E-02 2.20189E+01 5.07416E-02 0.00000E+00 0.00000E+00 2.50416E+01 5.80145E-02 10 3.62307E+01 1.21702E-01 2.13858E+01 7.94487E-02 0.00000E+00 0.00000E+00 2.50412E+01 8.59014E-02 11 3.52056E+01 1.83738E-01 2.08387E+01 1.26768E-01 0.00000E+00 0.00000E+00 2.50906E+01 1.33470E-01 12 3.38752E+01 2.75608E-01 2.06232E+01 2.04562E-01 0.00000E+00 0.00000E+00 2.51490E+01 2.05424E-01 13 3.24820E+01 4.36498E-01 2.05007E+01 3.22876E-01 0.00000E+00 0.00000E+00 2.47465E+01 3.21407E-01 14 3.38245E+01 6.86573E-01 1.97610E+01 5.59721E-01 0.00000E+00 0.00000E+00 2.60404E+01 5.49890E-01 15 3.05376E+01 1.02091E+00 1.86667E+01 7.63742E-01 0.00000E+00 0.00000E+00 2.40278E+01 7.93795E-01 16 3.36207E+01 1.43537E+00 1.91176E+01 1.41957E+00 0.00000E+00 0.00000E+00 2.53906E+01 1.39806E+00 17 3.23529E+01 1.69809E+00 2.74510E+01 3.61551E+00 --- --- 3.07190E+01 1.68509E+00 18 2.70833E+01 4.38420E+00 2.22222E+01 0.00000E+00 --- --- 2.61111E+01 3.51852E+00 19 2.63158E+01 0.00000E+00 --- --- --- --- 2.63158E+01 0.00000E+00 20 --- --- --- --- --- --- --- --- 21 --- --- --- --- --- --- --- --- 22 --- --- --- --- --- --- --- --- 23 --- --- --- --- --- --- --- --- 24 --- --- --- --- --- --- --- --- 25 --- --- --- --- --- --- --- --- 26 --- --- --- --- --- --- --- --- 27 --- --- --- --- --- --- --- --- 28 --- --- --- --- --- --- --- --- 29 --- --- --- --- --- --- --- --- 30 --- --- --- --- --- --- --- --- 31 --- --- --- --- --- --- --- --- 32 --- --- --- --- --- --- --- --- 33 --- --- --- --- --- --- --- --- 34 --- --- --- --- --- --- --- --- 35 --- --- --- --- --- --- --- --- 36 --- --- --- --- --- --- --- --- 37 --- --- --- --- --- --- --- --- 38 --- --- --- --- --- --- --- --- 39 --- --- --- --- --- --- --- --- 40 --- --- --- --- --- --- --- --- 41 --- --- --- --- --- --- --- --- 42 --- --- --- --- --- --- --- --- 43 --- --- --- --- --- --- --- --- 44 --- --- --- --- --- --- --- --- 45 --- --- --- --- --- --- --- --- 46 --- --- --- --- --- --- --- --- 47 --- --- --- --- --- --- --- --- 48 --- --- --- --- --- --- --- --- 49 --- --- --- --- --- --- --- --- 50 --- --- --- --- --- --- --- --- Average 7.60849E+01 8.56501E-03 7.19705E+01 2.61335E-03 0.00000E+00 0.00000E+00 2.50018E+01 9.47476E-04 ------------------------------------------------------------------------------------------------------------------- NOTES: (% Sb) is the percentage of lesions that are strand breaks. The number of Sb is ((% Sb)/100)*(# lesions). The number of Bd is (1-(% Sb)/100)*(# lesions). CLUSTER LENGTH (base pairs) =================================================================================================================== Number of --------- DSB ---------- --------- SSB ---------- -------- OTHER --------- ----- ALL CLUSTERS ----- lesions Average SEM Average SEM Average SEM Average SEM =================================================================================================================== 1 --- --- 1.00000E+00 0.00000E+00 1.00000E+00 0.00000E+00 1.00000E+00 0.00000E+00 2 5.36084E+00 1.78542E-03 5.51951E+00 4.76715E-04 5.50801E+00 4.05706E-04 5.50791E+00 3.03458E-04 3 9.43193E+00 2.64154E-03 1.01139E+01 9.97207E-04 1.00270E+01 1.08794E-03 1.00272E+01 7.06153E-04 4 1.37119E+01 4.39551E-03 1.47140E+01 1.89414E-03 1.45421E+01 2.56415E-03 1.45453E+01 1.42532E-03 5 1.81135E+01 7.68901E-03 1.92989E+01 3.50300E-03 1.90661E+01 5.90465E-03 1.90618E+01 2.72490E-03 6 2.25920E+01 1.34055E-02 2.38757E+01 6.62335E-03 2.35836E+01 1.36558E-02 2.35830E+01 5.17097E-03 7 2.71231E+01 2.10593E-02 2.84474E+01 1.29372E-02 2.80893E+01 2.62763E-02 2.81085E+01 1.01312E-02 8 3.16472E+01 3.30061E-02 3.30506E+01 2.19492E-02 3.26772E+01 5.14823E-02 3.26738E+01 1.82804E-02 9 3.61758E+01 5.32097E-02 3.76582E+01 3.63828E-02 3.71387E+01 1.02950E-01 3.71742E+01 2.99970E-02 10 4.07528E+01 8.57709E-02 4.21773E+01 6.25793E-02 4.15887E+01 2.13094E-01 4.16910E+01 5.01380E-02 11 4.51778E+01 1.43907E-01 4.65878E+01 1.09126E-01 4.66708E+01 4.33693E-01 4.60857E+01 8.61381E-02 12 4.94129E+01 2.32830E-01 5.16140E+01 1.83356E-01 5.02525E+01 8.15656E-01 5.07261E+01 1.43067E-01 13 5.45616E+01 4.03890E-01 5.55088E+01 3.25711E-01 5.55000E+01 1.77320E+00 5.51330E+01 2.51665E-01 14 5.95426E+01 6.31032E-01 5.99728E+01 6.30168E-01 5.78000E+01 2.95635E+00 5.97645E+01 4.43355E-01 15 6.36882E+01 1.01220E+00 6.29684E+01 1.14985E+00 6.92500E+01 7.69605E+00 6.34479E+01 7.64717E-01 16 6.56552E+01 1.49323E+00 6.78824E+01 2.21957E+00 7.00000E+01 0.00000E+00 6.69062E+01 1.35707E+00 17 7.40833E+01 1.19632E+00 6.78333E+01 5.81043E+00 --- --- 7.20000E+01 2.10663E+00 18 7.98750E+01 2.62840E+00 6.70000E+01 8.00000E+00 --- --- 7.73000E+01 2.94411E+00 19 9.10000E+01 0.00000E+00 --- --- --- --- 9.10000E+01 0.00000E+00 20 --- --- --- --- --- --- --- --- 21 --- --- --- --- --- --- --- --- 22 --- --- --- --- --- --- --- --- 23 --- --- --- --- --- --- --- --- 24 --- --- --- --- --- --- --- --- 25 --- --- --- --- --- --- --- --- 26 --- --- --- --- --- --- --- --- 27 --- --- --- --- --- --- --- --- 28 --- --- --- --- --- --- --- --- 29 --- --- --- --- --- --- --- --- 30 --- --- --- --- --- --- --- --- 31 --- --- --- --- --- --- --- --- 32 --- --- --- --- --- --- --- --- 33 --- --- --- --- --- --- --- --- 34 --- --- --- --- --- --- --- --- 35 --- --- --- --- --- --- --- --- 36 --- --- --- --- --- --- --- --- 37 --- --- --- --- --- --- --- --- 38 --- --- --- --- --- --- --- --- 39 --- --- --- --- --- --- --- --- 40 --- --- --- --- --- --- --- --- 41 --- --- --- --- --- --- --- --- 42 --- --- --- --- --- --- --- --- 43 --- --- --- --- --- --- --- --- 44 --- --- --- --- --- --- --- --- 45 --- --- --- --- --- --- --- --- 46 --- --- --- --- --- --- --- --- 47 --- --- --- --- --- --- --- --- 48 --- --- --- --- --- --- --- --- 49 --- --- --- --- --- --- --- --- 50 --- --- --- --- --- --- --- --- Average 1.06190E+01 2.75374E-03 5.05584E+00 5.25190E-04 2.73588E+00 2.31820E-04 3.65462E+00 2.28857E-04 ------------------------------------------------------------------------------------------------------------------- NUMBER OF LESIONS DIVIDED BY TWICE THE CLUSTER LENGTH (lesions per nucleotide) =================================================================================================================== Number of --------- DSB ---------- --------- SSB ---------- -------- OTHER --------- ----- ALL CLUSTERS ----- lesions Average SEM Average SEM Average SEM Average SEM =================================================================================================================== 1 --- --- 5.00000E-01 0.00000E+00 5.00000E-01 0.00000E+00 5.00000E-01 0.00000E+00 2 2.79886E-01 1.48314E-04 2.53353E-01 3.32257E-05 2.55295E-01 2.88031E-05 2.55304E-01 2.15420E-05 3 1.95080E-01 7.91661E-05 1.79515E-01 2.66377E-05 1.81520E-01 2.96538E-05 1.81504E-01 1.91747E-05 4 1.67640E-01 7.24845E-05 1.53967E-01 2.76751E-05 1.56291E-01 3.82509E-05 1.56262E-01 2.12912E-05 5 1.53014E-01 8.26478E-05 1.41844E-01 3.34003E-05 1.44074E-01 5.78259E-05 1.44087E-01 2.66183E-05 6 1.43962E-01 1.04427E-04 1.34923E-01 4.59928E-05 1.36953E-01 9.82163E-05 1.36991E-01 3.69914E-05 7 1.37771E-01 1.25846E-04 1.30479E-01 7.01469E-05 1.32420E-01 1.46747E-04 1.32346E-01 5.65923E-05 8 1.33595E-01 1.62027E-04 1.27183E-01 9.71827E-05 1.28979E-01 2.37960E-04 1.28933E-01 8.36699E-05 9 1.30490E-01 2.16417E-04 1.24695E-01 1.35784E-04 1.26783E-01 3.92011E-04 1.26596E-01 1.15591E-04 10 1.27885E-01 3.02432E-04 1.23241E-01 2.06384E-04 1.25184E-01 7.05963E-04 1.24830E-01 1.68840E-04 11 1.26489E-01 4.46934E-04 1.22259E-01 3.18763E-04 1.22303E-01 1.23064E-03 1.23785E-01 2.57627E-04 12 1.25592E-01 6.68912E-04 1.19607E-01 4.60988E-04 1.22636E-01 2.09591E-03 1.21997E-01 3.81768E-04 13 1.22675E-01 9.74398E-04 1.20326E-01 7.65014E-04 1.20858E-01 4.10824E-03 1.21272E-01 5.97593E-04 14 1.20617E-01 1.33118E-03 1.20290E-01 1.38862E-03 1.22342E-01 6.05156E-03 1.20439E-01 9.58589E-04 15 1.20896E-01 2.21880E-03 1.23039E-01 2.36533E-03 1.12522E-01 1.27826E-02 1.21782E-01 1.60621E-03 16 1.23584E-01 2.75272E-03 1.21769E-01 3.73232E-03 1.14286E-01 0.00000E+00 1.22474E-01 2.33069E-03 17 1.15066E-01 1.86294E-03 1.30453E-01 1.23398E-02 --- --- 1.20195E-01 4.41820E-03 18 1.13550E-01 3.80240E-03 1.36271E-01 1.62712E-02 --- --- 1.18094E-01 4.90484E-03 19 1.04396E-01 0.00000E+00 --- --- --- --- 1.04396E-01 0.00000E+00 20 --- --- --- --- --- --- --- --- 21 --- --- --- --- --- --- --- --- 22 --- --- --- --- --- --- --- --- 23 --- --- --- --- --- --- --- --- 24 --- --- --- --- --- --- --- --- 25 --- --- --- --- --- --- --- --- 26 --- --- --- --- --- --- --- --- 27 --- --- --- --- --- --- --- --- 28 --- --- --- --- --- --- --- --- 29 --- --- --- --- --- --- --- --- 30 --- --- --- --- --- --- --- --- 31 --- --- --- --- --- --- --- --- 32 --- --- --- --- --- --- --- --- 33 --- --- --- --- --- --- --- --- 34 --- --- --- --- --- --- --- --- 35 --- --- --- --- --- --- --- --- 36 --- --- --- --- --- --- --- --- 37 --- --- --- --- --- --- --- --- 38 --- --- --- --- --- --- --- --- 39 --- --- --- --- --- --- --- --- 40 --- --- --- --- --- --- --- --- 41 --- --- --- --- --- --- --- --- 42 --- --- --- --- --- --- --- --- 43 --- --- --- --- --- --- --- --- 44 --- --- --- --- --- --- --- --- 45 --- --- --- --- --- --- --- --- 46 --- --- --- --- --- --- --- --- 47 --- --- --- --- --- --- --- --- 48 --- --- --- --- --- --- --- --- 49 --- --- --- --- --- --- --- --- 50 --- --- --- --- --- --- --- --- Average 2.13534E-01 6.35128E-05 3.53067E-01 1.68787E-05 4.25651E-01 9.75103E-06 3.97624E-01 8.46516E-06 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