VC 2.00I 15-JUL-2003 01-AUG-2003 02:12:43 PM by RD Stewart (trebor@purdue.edu) PROGRAM FILE INPUT/OUTPUT AND RUN-TIME OPTIONS: ================================================================================ CMD: vc lpl.inp ..\xdat\c3h.xdat -c FNI: lpl.inp FNO: lpl.out RADX: ..\xdat\c3h.radx (7 exposures) XDAT: ..\xdat\c3h.xdat (7 experiments) -------------------------------------------------------------------------------- MCAT SUMMARY: BEST-FIT MODEL PARAMETERS (method = BCONF): ========================================================================================== --- PARAMETER --- ----- CONSTRAINTS ----- INITIAL OPTIMAL PID CID NAM Minimum Maximum GUESS VALUE 2001 CLRP BMR 0.00000E+00 1.00000E-02 1.00000E-04 1.3624112E-04 1101 IRAD FL 0.00000E+00 2.00000E+00 1.00000E-01 2.2766928E-01 ------------------------------------------------------------------------------------------ FIGURE OF MERIT (FOM): 5.21331E-03 (nfom=39, nsim=273, nmx=7, nox=7, ndf=4, nap=2) COMBINED UNWEIGHTED RESIDUAL: Stats: min=-6.554E-02 max=4.805E-02, mean=-1.5193E-02, stdev=4.4619E-02, var=1.9908E-03 Student's T test (mean=0, NDF=4): Pr>ABS(0.90092)= 4.1857E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.89975)=3.428E-01 (passed normality test) COMBINED WEIGHTED RESIDUAL: Stats: min=-1.196E-02 max=2.892E-02, mean=-1.3674E-03, stdev=1.4825E-02, var=2.1978E-04 Student's T test (mean=0, NDF=4): Pr>ABS(0.24403)= 8.1921E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.77744)=2.399E-02 (normality questionable) QECK TABLE: Cell and tissue kinetic parameters. ================================================================================ Duration of cell cycle (TCC): 10.00000 h Potential doubling time (Tpot): 480.00000 h (= 20.000 days) Cell birth rate (AX): 1.6636E+00 cell/day (= LOG(2)/TCC) Cell death rate (BX): 1.6289E+00 cell/day [=AX-LOG(2)/TPOT] Net cell birth rate (AX-BX): 3.4657E-02 cell/day Initial Number of Cells: non-dividing: 1.00000E+00 (100.000% = 1-GF) dividing: 0.00000E+00 ( 0.000% = GF) total: 1.00000E+00 (100.000% total) Life Support Capacity of System: Initial system capacity utilization: 0.000000% (=F0) Peak cell density (cells/cm^3): 1.00000E+38 (=KAP) Tissue volume (cm^3): 1.00000E+00 (=VOL) Maximum number of cells (cells): 1.00000E+38 (=KAP*VOL) -------------------------------------------------------------------------------- LPLM TABLE: Summary of LPL parameters and related (derived) quantities. ======================================================================== G0/G1 DNA CONTENT: 5667.000 Mbp/cell = 3.482938E+12 Da/cell ENDOGENOUS DAMAGE: per (h-Da) per (h-Mbp) per (h-cell) DSB = 0.00000E+00 0.00000E+00 0.00000E+00 RADIATION DAMAGE: per (Gy-Da) per (Gy-Mbp) per (Gy-cell) DSB = 7.17785E-12 4.41151E-03 25.000000 FL = 6.53670E-14 4.01746E-05 0.227669 DAMAGE REPAIR/MISREPAIR: A0= 9.500000E-01 (=95.000%) (1-A0)= 5.000000E-02 (=5.000%) RHT= 2.000000 h (=120.00 minute) LAM= 3.4657359E-01 (1/h) BMR= 1.3624112E-04 (1/h) LAM/BMR= 2.5438251E+03 GAM= 0.250 ***** EQUIVALENT LQ MODEL PARAMETERS ***** alpha = 0.227669 (1/Gy) beta = 0.030712 (1/Gy^2) alpha/beta = 7.413131 (Gy) ------------------------------------------------------------------------ NOTE: 1 bp = 614.6 Da (amu) = 1.0205E-09 pg MCAT FIT: CELL SURVIVAL AS A FUNCTION OF DOSE AND DOSE RATE (C3H 10T1/2 cells). ===================================================================================================== -------- EXPOSURE SUMMARY -------- Duration Dose Dose Rate ---------------- SURVIVING FRACTION -------------- EXPERIMENT (h) (Gy) (Gy/h)* Measured Norm. Wt Predicted Residual ----------------------------------------------------------------------------------------------------- 1 2.0833E-02 2.5000E-01 1.2000E+01 9.70000E-01 1.68E-01 9.42868E-01 -2.8776E-02 2 4.1667E-02 5.0000E-01 1.2000E+01 8.50000E-01 5.17E-01 8.85635E-01 4.0236E-02 3 8.3333E-02 1.0000E+00 1.2000E+01 8.00000E-01 1.14E-01 7.72680E-01 -3.5358E-02 4 1.2500E-01 1.5000E+00 1.2000E+01 6.70000E-01 1.43E-01 6.64340E-01 -8.5191E-03 5 1.6667E-01 2.0000E+00 1.2000E+01 6.00000E-01 2.86E-02 5.63092E-01 -6.5545E-02 6 2.5000E-01 3.0000E+00 1.2000E+01 4.10000E-01 1.34E-02 3.88094E-01 -5.6444E-02 7 4.1667E-01 5.0000E+00 1.2000E+01 1.50000E-01 1.61E-02 1.57572E-01 4.8052E-02 ----------------------------------------------------------------------------------------------------- RESIDUAL: Stats: min=-6.554E-02 max=4.805E-02, mean=-1.5193E-02, stdev=4.4619E-02, var=1.9908E-03 Student's T test (mean=0, NDF=4): Pr>ABS(0.90092)= 4.1857E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.89975)=3.428E-01 (passed normality test) WEIGHTED RESIDUAL: Stats: min=-1.196E-02 max=2.892E-02, mean=-1.3674E-03, stdev=1.4825E-02, var=2.1978E-04 Student's T test (mean=0, NDF=4): Pr>ABS(0.24403)= 8.1921E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.77744)=2.399E-02 (normality questionable) NOX=7, NPAR=2 CFE.SF: Cell survival data for C3H 10T1/2 cells. ========================================================================================= -------- EXPOSURE SUMMARY -------- ------------ ENDPOINT ------------- DATASET ID Duration Dose Dose Rate Fraction Fraction Standard CFE RADX (h) (Gy) (Gy/h) Killed Viable Error (%) ----------------------------------------------------------------------------------------- 1 1 2.0833E-02 2.5000E-01 1.2000E+01 3.00000E-02 9.70000E-01 4.12E-02 2 2 4.1667E-02 5.0000E-01 1.2000E+01 1.50000E-01 8.50000E-01 2.35E-02 3 3 8.3333E-02 1.0000E+00 1.2000E+01 2.00000E-01 8.00000E-01 5.00E-02 4 4 1.2500E-01 1.5000E+00 1.2000E+01 3.30000E-01 6.70000E-01 4.48E-02 5 5 1.6667E-01 2.0000E+00 1.2000E+01 4.00000E-01 6.00000E-01 1.00E-01 6 6 2.5000E-01 3.0000E+00 1.2000E+01 5.90000E-01 4.10000E-01 1.46E-01 7 7 4.1667E-01 5.0000E+00 1.2000E+01 8.50000E-01 1.50000E-01 1.33E-01 ----------------------------------------------------------------------------------------- CFE.DXA: Additional details from the cell survival assay (C3H 10T1/2). ===================================================================================== DATASET ID ---------- TIME (h) ----------- DOSE ---- LETHAL DAMAGE --- CFE RADX RADX TTP ETSI (Gy) #/(cell Gy) #/(cell) ------------------------------------------------------------------------------------- 1 1 2.083E-02 1.200E+01 4.000E+02 2.5000E-01 1.2184E-01 3.0459E-02 2 2 4.167E-02 1.200E+01 4.000E+02 5.0000E-01 3.2504E-01 1.6252E-01 3 3 8.333E-02 1.200E+01 4.000E+02 1.0000E+00 2.2314E-01 2.2314E-01 4 4 1.250E-01 1.200E+01 4.000E+02 1.5000E+00 2.6699E-01 4.0048E-01 5 5 1.667E-01 1.200E+01 4.000E+02 2.0000E+00 2.5541E-01 5.1083E-01 6 6 2.500E-01 1.200E+01 4.000E+02 3.0000E+00 2.9720E-01 8.9160E-01 7 7 4.167E-01 1.200E+01 4.000E+02 5.0000E+00 3.7942E-01 1.8971E+00 ------------------------------------------------------------------------------------- ETSI: Total elapsed time (after irradiation) before colonies are counted. TTP: Time available for repair before cells are plated. Total VC execution time (min): 1.25000E-02 VC 2.00I 15-JUL-2003 01-AUG-2003 02:12:43 PM