VC 2.00H 10-JUL-2003 23-JUL-2003 12:11:25 AM by RD Stewart (trebor@purdue.edu) PROGRAM FILE INPUT/OUTPUT AND RUN-TIME OPTIONS: ================================================================================ CMD: vc lpl.inp ..\xdat\hx118.xdat -c FNI: lpl.inp FNO: lpl.out RADX: ..\xdat\hx118.radx (22 exposures) XDAT: ..\xdat\hx118.xdat (22 experiments) -------------------------------------------------------------------------------- MCAT SUMMARY: BEST-FIT MODEL PARAMETERS (method = BCONF): ========================================================================================== --- PARAMETER --- ----- CONSTRAINTS ----- INITIAL OPTIMAL PID CID NAM Minimum Maximum GUESS VALUE 2001 CLRP BMR 0.00000E+00 1.00000E-02 1.00000E-04 1.2243103E-03 1101 IRAD FL 0.00000E+00 2.00000E+00 1.00000E-01 3.1442681E-01 2201 CLRP RHT 1.00000E-02 1.20000E+01 2.00000E+00 2.8291406E-01 ------------------------------------------------------------------------------------------ FIGURE OF MERIT (FOM): 9.33537E-03 (nfom=288, nsim=6336, nmx=22, nox=22, ndf=18, nap=3) COMBINED UNWEIGHTED RESIDUAL: Stats: min=-3.010E-01 max=4.939E-01, mean=1.3841E-02, stdev=2.0973E-01, var=4.3988E-02 Student's T test (NDF=18): Pr>ABS(0.30954)= 7.6047E-01 (accept fit) Shapiro-Wilk's Test: (Pr<0.94902)=3.002E-01 (accept normality) COMBINED WEIGHTED RESIDUAL: Stats: min=-6.417E-02 max=1.053E-01, mean=2.9510E-03, stdev=4.4715E-02, var=1.9995E-03 Student's T test (NDF=18): Pr>ABS(0.30954)= 7.6047E-01 (accept fit) Shapiro-Wilk's Test: (Pr<0.94902)=3.002E-01 (accept normality) QECK TABLE: Cell and tissue kinetic parameters. ================================================================================ Duration of cell cycle (TCC): 10.00000 h Potential doubling time (Tpot): 480.00000 h (= 20.000 days) Cell birth rate (AX): 1.6636E+00 cell/day (= LOG(2)/TCC) Cell death rate (BX): 1.6289E+00 cell/day [=AX-LOG(2)/TPOT] Net cell birth rate (AX-BX): 3.4657E-02 cell/day Initial Number of Cells: non-dividing: 1.00000E+00 (100.000% = 1-GF) dividing: 0.00000E+00 ( 0.000% = GF) total: 1.00000E+00 (100.000% total) Life Support Capacity of System: Initial system capacity utilization: 0.000000% (=F0) Peak cell density (cells/cm^3): 1.00000E+38 (=KAP) Tissue volume (cm^3): 1.00000E+00 (=VOL) Maximum number of cells (cells): 1.00000E+38 (=KAP*VOL) -------------------------------------------------------------------------------- LPLM TABLE: Summary of LPL parameters and related (derived) quantities. ======================================================================== G0/G1 DNA CONTENT: 5667.000 Mbp/cell = 3.482938E+12 Da/cell ENDOGENOUS DAMAGE: per (h-Da) per (h-Mbp) per (h-cell) DSB = 0.00000E+00 0.00000E+00 0.00000E+00 RADIATION DAMAGE: per (Gy-Da) per (Gy-Mbp) per (Gy-cell) DSB = 7.17785E-12 4.41151E-03 25.000000 FL = 9.02763E-14 5.54838E-05 0.314427 DAMAGE REPAIR/MISREPAIR: A0= 9.500000E-01 (=95.000%) (1-A0)= 5.000000E-02 (=5.000%) RHT= 0.282914 h (=16.97 minute) LAM= 2.4500274E+00 (1/h) BMR= 1.2243103E-03 (1/h) LAM/BMR= 2.0011490E+03 GAM= 0.250 ***** EQUIVALENT LQ MODEL PARAMETERS ***** alpha = 0.314427 (1/Gy) beta = 0.039040 (1/Gy^2) alpha/beta = 8.053951 (Gy) ------------------------------------------------------------------------ NOTE: 1 bp = 614.6 Da (amu) = 1.0205E-09 pg MCAT FIT: CELL SURVIVAL AS A FUNCTION OF DOSE AND DOSE RATE (HX 118 cells). ===================================================================================================== -------- EXPOSURE SUMMARY -------- Duration Dose Dose Rate ---------------- SURVIVING FRACTION -------------- EXPERIMENT (h) (Gy) (Gy/h)* Measured Norm. Wt Predicted Residual ----------------------------------------------------------------------------------------------------- 1 1.9896E+00 1.9100E+00 9.6000E-01 6.00870E-01 4.55E-02 5.23743E-01 -1.4726E-01 2 3.0521E+00 2.9300E+00 9.6000E-01 3.80573E-01 4.55E-02 3.68508E-01 -3.2741E-02 3 4.2708E+00 4.1000E+00 9.6000E-01 2.91696E-01 4.55E-02 2.46199E-01 -1.8480E-01 4 5.1354E+00 4.9300E+00 9.6000E-01 2.16803E-01 4.55E-02 1.84937E-01 -1.7231E-01 5 6.1771E+00 5.9300E+00 9.6000E-01 1.56318E-01 4.55E-02 1.31012E-01 -1.9315E-01 6 7.2396E+00 6.9500E+00 9.6000E-01 7.58190E-02 4.55E-02 9.21735E-02 1.7743E-01 7 8.3021E+00 7.9700E+00 9.6000E-01 6.81950E-02 4.55E-02 6.48484E-02 -5.1606E-02 8 1.1458E+01 1.1000E+01 9.6000E-01 1.31680E-02 4.55E-02 2.28177E-02 4.2290E-01 9 1.5719E+01 1.5090E+01 9.6000E-01 5.33100E-03 4.55E-02 5.57119E-03 4.3113E-02 10 2.0175E-01 9.2000E-01 4.5600E+00 7.54728E-01 4.55E-02 7.28123E-01 -3.6540E-02 11 4.1228E-01 1.8800E+00 4.5600E+00 5.35921E-01 4.55E-02 5.01055E-01 -6.9585E-02 12 8.7061E-01 3.9700E+00 4.5600E+00 2.68203E-01 4.55E-02 2.06151E-01 -3.0101E-01 13 1.3070E+00 5.9600E+00 4.5600E+00 1.05961E-01 4.55E-02 8.51411E-02 -2.4453E-01 14 1.7478E+00 7.9700E+00 4.5600E+00 3.45980E-02 4.55E-02 3.44303E-02 -4.8714E-03 15 2.1952E+00 1.0010E+01 4.5600E+00 1.24740E-02 4.55E-02 1.36809E-02 8.8215E-02 16 2.6425E+00 1.2050E+01 4.5600E+00 4.85400E-03 4.55E-02 5.42898E-03 1.0591E-01 17 1.0000E-02 9.0000E-01 9.0000E+01 6.78309E-01 4.55E-02 7.30433E-01 7.1360E-02 18 2.0889E-02 1.8800E+00 9.0000E+01 4.01119E-01 4.55E-02 4.84464E-01 1.7204E-01 19 4.4333E-02 3.9900E+00 9.0000E+01 1.49109E-01 4.55E-02 1.59565E-01 6.5528E-02 20 6.6222E-02 5.9600E+00 9.0000E+01 3.05780E-02 4.55E-02 4.37858E-02 3.0165E-01 21 8.9000E-02 8.0100E+00 9.0000E+01 4.55300E-03 4.55E-02 8.99679E-03 4.9393E-01 22 1.1167E-01 1.0050E+01 9.0000E+01 1.79900E-03 4.55E-02 1.50021E-03 -1.9916E-01 ----------------------------------------------------------------------------------------------------- RESIDUAL: Stats: min=-3.010E-01 max=4.939E-01, mean=1.3841E-02, stdev=2.0973E-01, var=4.3988E-02 Student's T test (NDF=18): Pr>ABS(0.30954)= 7.6047E-01 (accept fit) Shapiro-Wilk's Test: (Pr<0.94902)=3.002E-01 (accept normality) WEIGHTED RESIDUAL: Stats: min=-6.417E-02 max=1.053E-01, mean=2.9510E-03, stdev=4.4715E-02, var=1.9995E-03 Student's T test (NDF=18): Pr>ABS(0.30954)= 7.6047E-01 (accept fit) Shapiro-Wilk's Test: (Pr<0.94902)=3.002E-01 (accept normality) NOX=22, NPAR=3 CFE.SF: Cell survival data for HX 118 cells. ========================================================================================= -------- EXPOSURE SUMMARY -------- ------------ ENDPOINT ------------- DATASET ID Duration Dose Dose Rate Fraction Fraction Standard CFE RADX (h) (Gy) (Gy/h) Killed Viable Error (%) ----------------------------------------------------------------------------------------- 1 1 1.9896E+00 1.9100E+00 9.6000E-01 3.99130E-01 6.00870E-01 1.00E+01 2 2 3.0521E+00 2.9300E+00 9.6000E-01 6.19427E-01 3.80573E-01 1.00E+01 3 3 4.2708E+00 4.1000E+00 9.6000E-01 7.08304E-01 2.91696E-01 1.00E+01 4 4 5.1354E+00 4.9300E+00 9.6000E-01 7.83197E-01 2.16803E-01 1.00E+01 5 5 6.1771E+00 5.9300E+00 9.6000E-01 8.43682E-01 1.56318E-01 1.00E+01 6 6 7.2396E+00 6.9500E+00 9.6000E-01 9.24181E-01 7.58190E-02 1.00E+01 7 7 8.3021E+00 7.9700E+00 9.6000E-01 9.31805E-01 6.81950E-02 1.00E+01 8 8 1.1458E+01 1.1000E+01 9.6000E-01 9.86832E-01 1.31680E-02 1.00E+01 9 9 1.5719E+01 1.5090E+01 9.6000E-01 9.94669E-01 5.33100E-03 1.00E+01 10 10 2.0175E-01 9.2000E-01 4.5600E+00 2.45272E-01 7.54728E-01 1.00E+01 11 11 4.1228E-01 1.8800E+00 4.5600E+00 4.64079E-01 5.35921E-01 1.00E+01 12 12 8.7061E-01 3.9700E+00 4.5600E+00 7.31797E-01 2.68203E-01 1.00E+01 13 13 1.3070E+00 5.9600E+00 4.5600E+00 8.94039E-01 1.05961E-01 1.00E+01 14 14 1.7478E+00 7.9700E+00 4.5600E+00 9.65402E-01 3.45980E-02 1.00E+01 15 15 2.1952E+00 1.0010E+01 4.5600E+00 9.87526E-01 1.24740E-02 1.00E+01 16 16 2.6425E+00 1.2050E+01 4.5600E+00 9.95146E-01 4.85400E-03 1.00E+01 17 17 1.0000E-02 9.0000E-01 9.0000E+01 3.21691E-01 6.78309E-01 1.00E+01 18 18 2.0889E-02 1.8800E+00 9.0000E+01 5.98881E-01 4.01119E-01 1.00E+01 19 19 4.4333E-02 3.9900E+00 9.0000E+01 8.50891E-01 1.49109E-01 1.00E+01 20 20 6.6222E-02 5.9600E+00 9.0000E+01 9.69422E-01 3.05780E-02 1.00E+01 21 21 8.9000E-02 8.0100E+00 9.0000E+01 9.95447E-01 4.55300E-03 1.00E+01 22 22 1.1167E-01 1.0050E+01 9.0000E+01 9.98201E-01 1.79900E-03 1.00E+01 ----------------------------------------------------------------------------------------- CFE.DXA: Additional details from the cell survival assay (HX 118). ===================================================================================== DATASET ID ---------- TIME (h) ----------- DOSE ---- LETHAL DAMAGE --- CFE RADX RADX TTP ETSI (Gy) #/(cell Gy) #/(cell) ------------------------------------------------------------------------------------- 1 1 1.990E+00 1.200E+01 4.000E+02 1.9100E+00 2.6669E-01 5.0938E-01 2 2 3.052E+00 1.200E+01 4.000E+02 2.9300E+00 3.2972E-01 9.6608E-01 3 3 4.271E+00 1.200E+01 4.000E+02 4.1000E+00 3.0050E-01 1.2320E+00 4 4 5.135E+00 1.200E+01 4.000E+02 4.9300E+00 3.1009E-01 1.5288E+00 5 5 6.177E+00 1.200E+01 4.000E+02 5.9300E+00 3.1296E-01 1.8559E+00 6 6 7.240E+00 1.200E+01 4.000E+02 6.9500E+00 3.7114E-01 2.5794E+00 7 7 8.302E+00 1.200E+01 4.000E+02 7.9700E+00 3.3694E-01 2.6854E+00 8 8 1.146E+01 1.200E+01 4.000E+02 1.1000E+01 3.9363E-01 4.3300E+00 9 9 1.572E+01 1.200E+01 4.000E+02 1.5090E+01 3.4687E-01 5.2342E+00 10 10 2.018E-01 1.200E+01 4.000E+02 9.2000E-01 3.0587E-01 2.8140E-01 11 11 4.123E-01 1.200E+01 4.000E+02 1.8800E+00 3.3179E-01 6.2377E-01 12 12 8.706E-01 1.200E+01 4.000E+02 3.9700E+00 3.3149E-01 1.3160E+00 13 13 1.307E+00 1.200E+01 4.000E+02 5.9600E+00 3.7662E-01 2.2447E+00 14 14 1.748E+00 1.200E+01 4.000E+02 7.9700E+00 4.2208E-01 3.3640E+00 15 15 2.195E+00 1.200E+01 4.000E+02 1.0010E+01 4.3797E-01 4.3841E+00 16 16 2.643E+00 1.200E+01 4.000E+02 1.2050E+01 4.4215E-01 5.3280E+00 17 17 1.000E-02 1.200E+01 4.000E+02 9.0000E-01 4.3128E-01 3.8815E-01 18 18 2.089E-02 1.200E+01 4.000E+02 1.8800E+00 4.8590E-01 9.1350E-01 19 19 4.433E-02 1.200E+01 4.000E+02 3.9900E+00 4.7696E-01 1.9031E+00 20 20 6.622E-02 1.200E+01 4.000E+02 5.9600E+00 5.8515E-01 3.4875E+00 21 21 8.900E-02 1.200E+01 4.000E+02 8.0100E+00 6.7315E-01 5.3920E+00 22 22 1.117E-01 1.200E+01 4.000E+02 1.0050E+01 6.2891E-01 6.3205E+00 ------------------------------------------------------------------------------------- ETSI: Total elapsed time (after irradiation) before colonies are counted. TTP: Time available for repair before cells are plated. Total VC execution time (min): 0.28515 VC 2.00H 10-JUL-2003 23-JUL-2003 12:11:43 AM