VC 2.00J 25-SEP-2003 20-NOV-2003 10:22:03 AM by RD Stewart (trebor@purdue.edu) PROGRAM FILE INPUT/OUTPUT AND RUN-TIME OPTIONS: ================================================================================ CMD: vc lpl.inp ..\xdat\ppc1.xdat -c FNI: lpl.inp FNO: lpl.out RADX: ..\xdat\ppc1.radx (8 exposures) XDAT: ..\xdat\ppc1.xdat (8 experiments) -------------------------------------------------------------------------------- MCAT SUMMARY: BEST-FIT MODEL PARAMETERS (method = BCONF): ========================================================================================== --- PARAMETER --- ----- CONSTRAINTS ----- INITIAL OPTIMAL PID CID NAM Minimum Maximum GUESS VALUE 2001 CLRP BMR 0.00000E+00 1.00000E+00 1.00000E-04 5.1941386E-05 1101 IRAD FL 0.00000E+00 2.00000E+00 1.00000E-01 1.0925695E-01 2201 CLRP RHT 1.00000E-02 2.40000E+01 8.40000E+00 9.0982871E+00 ------------------------------------------------------------------------------------------ FIGURE OF MERIT (FOM): 9.99475E-03 (nfom=3178, nsim=25424, nmx=8, nox=8, ndf=4, nap=3) COMBINED UNWEIGHTED RESIDUAL: Stats: min=-9.345E-02 max=1.876E-01, mean=8.1479E-03, stdev=8.5034E-02, var=7.2307E-03 Student's T test (mean=0, NDF=4): Pr>ABS(0.27102)= 7.9979E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.86977)=1.528E-01 (passed normality test) COMBINED WEIGHTED RESIDUAL: Stats: min=-3.304E-02 max=6.634E-02, mean=2.8807E-03, stdev=3.0064E-02, var=9.0384E-04 Student's T test (mean=0, NDF=4): Pr>ABS(0.27102)= 7.9979E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.86977)=1.528E-01 (passed normality test) QECK TABLE: Cell and tissue kinetic parameters. ================================================================================ Duration of cell cycle (TCC): 10.00000 h Potential doubling time (Tpot): 480.00000 h (= 20.000 days) Cell birth rate (AX): 1.6636E+00 cell/day (= LOG(2)/TCC) Cell death rate (BX): 1.6289E+00 cell/day [=AX-LOG(2)/TPOT] Net cell birth rate (AX-BX): 3.4657E-02 cell/day Initial Number of Cells: non-dividing: 1.00000E+00 (100.000% = 1-GF) dividing: 0.00000E+00 ( 0.000% = GF) total: 1.00000E+00 (100.000% total) Life Support Capacity of System: Initial system capacity utilization: 0.000000% (=F0) Peak cell density (cells/cm^3): 1.00000E+38 (=KAP) Tissue volume (cm^3): 1.00000E+00 (=VOL) Maximum number of cells (cells): 1.00000E+38 (=KAP*VOL) -------------------------------------------------------------------------------- LPLM TABLE: Summary of LPL parameters and related (derived) quantities. ======================================================================== G0/G1 DNA CONTENT: 5667.000 Mbp/cell = 3.482938E+12 Da/cell ENDOGENOUS DAMAGE: per (h-Da) per (h-Mbp) per (h-cell) DSB = 0.00000E+00 0.00000E+00 0.00000E+00 RADIATION DAMAGE: per (Gy-Da) per (Gy-Mbp) per (Gy-cell) DSB = 8.61342E-12 5.29381E-03 30.000000 FL = 3.13692E-14 1.92795E-05 0.109257 DAMAGE REPAIR/MISREPAIR: A0= 9.500000E-01 (=95.000%) (1-A0)= 5.000000E-02 (=5.000%) RHT= 9.098287 h (=545.90 minute) LAM= 7.6184360E-02 (1/h) BMR= 5.1941386E-05 (1/h) LAM/BMR= 1.4667371E+03 GAM= 0.250 ***** EQUIVALENT LQ MODEL PARAMETERS ***** alpha = 0.109257 (1/Gy) beta = 0.076701 (1/Gy^2) alpha/beta = 1.424455 (Gy) ------------------------------------------------------------------------ NOTE: 1 bp = 614.6 Da (amu) = 1.0205E-09 pg MCAT FIT: CELL SURVIVAL AS A FUNCTION OF DOSE AND DOSE RATE (PPC1 cells). ===================================================================================================== -------- EXPOSURE SUMMARY -------- Duration Dose Dose Rate ---------------- SURVIVING FRACTION -------------- EXPERIMENT (h) (Gy) (Gy/h)* Measured Norm. Wt Predicted Residual ----------------------------------------------------------------------------------------------------- 1 2.4040E+01 6.0100E+00 2.5000E-01 1.03048E-01 1.25E-01 1.05076E-01 1.9302E-02 2 4.7872E+01 1.1968E+01 2.5000E-01 4.63300E-03 1.25E-01 4.42333E-03 -4.7400E-02 3 7.1772E+01 1.7943E+01 2.5000E-01 1.60000E-04 1.25E-01 1.63761E-04 2.2963E-02 4 3.3580E-02 2.0148E+00 6.0000E+01 5.54660E-01 1.25E-01 5.70311E-01 2.7443E-02 5 6.6613E-02 3.9968E+00 6.0000E+01 1.86990E-01 1.25E-01 1.88103E-01 5.9182E-03 6 9.9722E-02 5.9833E+00 6.0000E+01 4.05600E-02 1.25E-01 3.70937E-02 -9.3446E-02 7 1.3289E-01 7.9735E+00 6.0000E+01 3.68000E-03 1.25E-01 4.52995E-03 1.8763E-01 8 1.6624E-01 9.9746E+00 6.0000E+01 3.70000E-04 1.25E-01 3.49973E-04 -5.7226E-02 ----------------------------------------------------------------------------------------------------- RESIDUAL: Stats: min=-9.345E-02 max=1.876E-01, mean=8.1479E-03, stdev=8.5034E-02, var=7.2307E-03 Student's T test (mean=0, NDF=4): Pr>ABS(0.27102)= 7.9979E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.86977)=1.528E-01 (passed normality test) WEIGHTED RESIDUAL: Stats: min=-3.304E-02 max=6.634E-02, mean=2.8807E-03, stdev=3.0064E-02, var=9.0384E-04 Student's T test (mean=0, NDF=4): Pr>ABS(0.27102)= 7.9979E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.86977)=1.528E-01 (passed normality test) NOX=8, NPAR=3 CFE.SF: Cell survival data for PPC1 cells. ========================================================================================= -------- EXPOSURE SUMMARY -------- ------------ ENDPOINT ------------- DATASET ID Duration Dose Dose Rate Fraction Fraction Standard CFE RADX (h) (Gy) (Gy/h) Killed Viable Error (%) ----------------------------------------------------------------------------------------- 1 1 2.4040E+01 6.0100E+00 2.5000E-01 8.96952E-01 1.03048E-01 1.00E+01 2 2 4.7872E+01 1.1968E+01 2.5000E-01 9.95367E-01 4.63300E-03 1.00E+01 3 3 7.1772E+01 1.7943E+01 2.5000E-01 9.99840E-01 1.60000E-04 1.00E+01 4 4 3.3580E-02 2.0148E+00 6.0000E+01 4.45340E-01 5.54660E-01 1.00E+01 5 5 6.6613E-02 3.9968E+00 6.0000E+01 8.13010E-01 1.86990E-01 1.00E+01 6 6 9.9722E-02 5.9833E+00 6.0000E+01 9.59440E-01 4.05600E-02 1.00E+01 7 7 1.3289E-01 7.9735E+00 6.0000E+01 9.96320E-01 3.68000E-03 1.00E+01 8 8 1.6624E-01 9.9746E+00 6.0000E+01 9.99630E-01 3.70000E-04 1.00E+01 ----------------------------------------------------------------------------------------- CFE.DXA: Additional details from the cell survival assay (PPC1). ===================================================================================== DATASET ID ---------- TIME (h) ----------- DOSE ---- LETHAL DAMAGE --- CFE RADX RADX TTP ETSI (Gy) #/(cell Gy) #/(cell) ------------------------------------------------------------------------------------- 1 1 2.404E+01 1.200E+01 4.000E+02 6.0100E+00 3.7813E-01 2.2726E+00 2 2 4.787E+01 1.200E+01 4.000E+02 1.1968E+01 4.4908E-01 5.3746E+00 3 3 7.177E+01 1.200E+01 4.000E+02 1.7943E+01 4.8712E-01 8.7403E+00 4 4 3.358E-02 1.200E+01 4.000E+02 2.0148E+00 2.9254E-01 5.8940E-01 5 5 6.661E-02 1.200E+01 4.000E+02 3.9968E+00 4.1951E-01 1.6767E+00 6 6 9.972E-02 1.200E+01 4.000E+02 5.9833E+00 5.3565E-01 3.2050E+00 7 7 1.329E-01 1.200E+01 4.000E+02 7.9735E+00 7.0293E-01 5.6048E+00 8 8 1.662E-01 1.200E+01 4.000E+02 9.9746E+00 7.9221E-01 7.9020E+00 ------------------------------------------------------------------------------------- ETSI: Total elapsed time (after irradiation) before colonies are counted. TTP: Time available for repair before cells are plated. Total VC execution time (min): 1.2946 VC 2.00J 25-SEP-2003 20-NOV-2003 10:23:21 AM