VC 2.00J 25-SEP-2003 20-NOV-2003 09:02:22 PM by RD Stewart (trebor@purdue.edu) PROGRAM FILE INPUT/OUTPUT AND RUN-TIME OPTIONS: ================================================================================ CMD: vc lpl.inp ..\xdat\pc3.xdat -c FNI: lpl.inp FNO: lpl.out RADX: ..\xdat\pc3.radx (6 exposures) XDAT: ..\xdat\pc3.xdat (6 experiments) -------------------------------------------------------------------------------- MCAT SUMMARY: BEST-FIT MODEL PARAMETERS (method = BCONF): ========================================================================================== --- PARAMETER --- ----- CONSTRAINTS ----- INITIAL OPTIMAL PID CID NAM Minimum Maximum GUESS VALUE 2001 CLRP BMR 0.00000E+00 1.00000E+00 1.00000E-04 5.1227824E-05 1101 IRAD FL 0.00000E+00 2.00000E+00 4.00000E-01 3.8469922E-01 ------------------------------------------------------------------------------------------ FIGURE OF MERIT (FOM): 1.61726E-02 (nfom=277, nsim=1662, nmx=6, nox=6, ndf=3, nap=2) COMBINED UNWEIGHTED RESIDUAL: Stats: min=-1.531E-01 max=1.666E-01, mean=8.4941E-03, stdev=1.0589E-01, var=1.1213E-02 Student's T test (mean=0, NDF=3): Pr>ABS(0.19649)= 8.5678E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.95065)=7.632E-01 (passed normality test) COMBINED WEIGHTED RESIDUAL: Stats: min=-6.250E-02 max=6.802E-02, mean=3.4677E-03, stdev=4.3229E-02, var=1.8688E-03 Student's T test (mean=0, NDF=3): Pr>ABS(0.19649)= 8.5678E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.95065)=7.632E-01 (passed normality test) QECK TABLE: Cell and tissue kinetic parameters. ================================================================================ Duration of cell cycle (TCC): 10.00000 h Potential doubling time (Tpot): 480.00000 h (= 20.000 days) Cell birth rate (AX): 1.6636E+00 cell/day (= LOG(2)/TCC) Cell death rate (BX): 1.6289E+00 cell/day [=AX-LOG(2)/TPOT] Net cell birth rate (AX-BX): 3.4657E-02 cell/day Initial Number of Cells: non-dividing: 1.00000E+00 (100.000% = 1-GF) dividing: 0.00000E+00 ( 0.000% = GF) total: 1.00000E+00 (100.000% total) Life Support Capacity of System: Initial system capacity utilization: 0.000000% (=F0) Peak cell density (cells/cm^3): 1.00000E+38 (=KAP) Tissue volume (cm^3): 1.00000E+00 (=VOL) Maximum number of cells (cells): 1.00000E+38 (=KAP*VOL) -------------------------------------------------------------------------------- LPLM TABLE: Summary of LPL parameters and related (derived) quantities. ======================================================================== G0/G1 DNA CONTENT: 5667.000 Mbp/cell = 3.482938E+12 Da/cell ENDOGENOUS DAMAGE: per (h-Da) per (h-Mbp) per (h-cell) DSB = 0.00000E+00 0.00000E+00 0.00000E+00 RADIATION DAMAGE: per (Gy-Da) per (Gy-Mbp) per (Gy-cell) DSB = 1.14846E-11 7.05841E-03 40.000000 FL = 1.10453E-13 6.78841E-05 0.384699 DAMAGE REPAIR/MISREPAIR: A0= 9.500000E-01 (=95.000%) (1-A0)= 5.000000E-02 (=5.000%) RHT= 6.000000 h (=360.00 minute) LAM= 1.1552453E-01 (1/h) BMR= 5.1227824E-05 (1/h) LAM/BMR= 2.2551130E+03 GAM= 0.250 ***** EQUIVALENT LQ MODEL PARAMETERS ***** alpha = 0.384699 (1/Gy) beta = 0.088687 (1/Gy^2) alpha/beta = 4.337701 (Gy) ------------------------------------------------------------------------ NOTE: 1 bp = 614.6 Da (amu) = 1.0205E-09 pg MCAT FIT: CELL SURVIVAL AS A FUNCTION OF DOSE AND DOSE RATE (PC3 cells). ===================================================================================================== -------- EXPOSURE SUMMARY -------- Duration Dose Dose Rate ---------------- SURVIVING FRACTION -------------- EXPERIMENT (h) (Gy) (Gy/h)* Measured Norm. Wt Predicted Residual ----------------------------------------------------------------------------------------------------- 1 1.7100E-02 1.0260E+00 6.0000E+01 6.45343E-01 1.67E-01 6.14162E-01 -5.0771E-02 2 3.3767E-02 2.0260E+00 6.0000E+01 3.08808E-01 1.67E-01 3.21205E-01 3.8595E-02 3 4.9867E-02 2.9920E+00 6.0000E+01 1.22397E-01 1.67E-01 1.46868E-01 1.6662E-01 4 6.6200E-02 3.9720E+00 6.0000E+01 6.57900E-02 1.67E-01 5.70558E-02 -1.5308E-01 5 8.3367E-02 5.0020E+00 6.0000E+01 1.75520E-02 1.67E-01 1.80178E-02 2.5852E-02 6 1.0008E-01 6.0050E+00 6.0000E+01 4.92200E-03 1.67E-01 5.04175E-03 2.3751E-02 ----------------------------------------------------------------------------------------------------- RESIDUAL: Stats: min=-1.531E-01 max=1.666E-01, mean=8.4941E-03, stdev=1.0589E-01, var=1.1213E-02 Student's T test (mean=0, NDF=3): Pr>ABS(0.19649)= 8.5678E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.95065)=7.632E-01 (passed normality test) WEIGHTED RESIDUAL: Stats: min=-6.250E-02 max=6.802E-02, mean=3.4677E-03, stdev=4.3229E-02, var=1.8688E-03 Student's T test (mean=0, NDF=3): Pr>ABS(0.19649)= 8.5678E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.95065)=7.632E-01 (passed normality test) NOX=6, NPAR=2 CFE.SF: Cell survival data for PC3 cells. ========================================================================================= -------- EXPOSURE SUMMARY -------- ------------ ENDPOINT ------------- DATASET ID Duration Dose Dose Rate Fraction Fraction Standard CFE RADX (h) (Gy) (Gy/h) Killed Viable Error (%) ----------------------------------------------------------------------------------------- 1 1 1.7100E-02 1.0260E+00 6.0000E+01 3.54657E-01 6.45343E-01 1.00E+01 2 2 3.3767E-02 2.0260E+00 6.0000E+01 6.91192E-01 3.08808E-01 1.00E+01 3 3 4.9867E-02 2.9920E+00 6.0000E+01 8.77603E-01 1.22397E-01 1.00E+01 4 4 6.6200E-02 3.9720E+00 6.0000E+01 9.34210E-01 6.57900E-02 1.00E+01 5 5 8.3367E-02 5.0020E+00 6.0000E+01 9.82448E-01 1.75520E-02 1.00E+01 6 6 1.0008E-01 6.0050E+00 6.0000E+01 9.95078E-01 4.92200E-03 1.00E+01 ----------------------------------------------------------------------------------------- CFE.DXA: Additional details from the cell survival assay (PC3). ===================================================================================== DATASET ID ---------- TIME (h) ----------- DOSE ---- LETHAL DAMAGE --- CFE RADX RADX TTP ETSI (Gy) #/(cell Gy) #/(cell) ------------------------------------------------------------------------------------- 1 1 1.710E-02 1.200E+01 4.000E+02 1.0260E+00 4.2687E-01 4.3797E-01 2 2 3.377E-02 1.200E+01 4.000E+02 2.0260E+00 5.7998E-01 1.1750E+00 3 3 4.987E-02 1.200E+01 4.000E+02 2.9920E+00 7.0203E-01 2.1005E+00 4 4 6.620E-02 1.200E+01 4.000E+02 3.9720E+00 6.8512E-01 2.7213E+00 5 5 8.337E-02 1.200E+01 4.000E+02 5.0020E+00 8.0819E-01 4.0426E+00 6 6 1.001E-01 1.200E+01 4.000E+02 6.0050E+00 8.8494E-01 5.3140E+00 ------------------------------------------------------------------------------------- ETSI: Total elapsed time (after irradiation) before colonies are counted. TTP: Time available for repair before cells are plated. Total VC execution time (min): 8.77167E-02 VC 2.00J 25-SEP-2003 20-NOV-2003 09:02:27 PM