VC 2.00J 25-SEP-2003 19-NOV-2003 10:29:57 AM by RD Stewart (trebor@purdue.edu) PROGRAM FILE INPUT/OUTPUT AND RUN-TIME OPTIONS: ================================================================================ CMD: vc rmr.inp ..\xdat\pc3b.xdat -c FNI: rmr.inp FNO: rmr.out RADX: ..\xdat\pc3b.radx (7 exposures) XDAT: ..\xdat\pc3b.xdat (7 experiments) -------------------------------------------------------------------------------- MCAT SUMMARY: BEST-FIT MODEL PARAMETERS (method = BCONF): ========================================================================================== --- PARAMETER --- ----- CONSTRAINTS ----- INITIAL OPTIMAL PID CID NAM Minimum Maximum GUESS VALUE 2001 CLRP BMR 0.00000E+00 1.00000E-02 1.00000E-04 1.4836442E-04 2401 CLRP PHI 0.00000E+00 1.00000E+00 1.00000E-02 5.5433341E-03 ------------------------------------------------------------------------------------------ FIGURE OF MERIT (FOM): 6.91621E-03 (nfom=87, nsim=609, nmx=7, nox=7, ndf=4, nap=2) COMBINED UNWEIGHTED RESIDUAL: Stats: min=-6.038E-02 max=8.403E-02, mean=-9.1203E-04, stdev=5.2283E-02, var=2.7335E-03 Student's T test (mean=0, NDF=4): Pr>ABS(0.04615)= 9.6540E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.94565)=7.062E-01 (passed normality test) COMBINED WEIGHTED RESIDUAL: Stats: min=-2.282E-02 max=3.176E-02, mean=-3.4472E-04, stdev=1.9761E-02, var=3.9050E-04 Student's T test (mean=0, NDF=4): Pr>ABS(0.04615)= 9.6540E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.94565)=7.062E-01 (passed normality test) QECK TABLE: Cell and tissue kinetic parameters. ================================================================================ Duration of cell cycle (TCC): 10.00000 h Potential doubling time (Tpot): 480.00000 h (= 20.000 days) Cell birth rate (AX): 1.6636E+00 cell/day (= LOG(2)/TCC) Cell death rate (BX): 1.6289E+00 cell/day [=AX-LOG(2)/TPOT] Net cell birth rate (AX-BX): 3.4657E-02 cell/day Initial Number of Cells: non-dividing: 1.00000E+00 (100.000% = 1-GF) dividing: 0.00000E+00 ( 0.000% = GF) total: 1.00000E+00 (100.000% total) Life Support Capacity of System: Initial system capacity utilization: 0.000000% (=F0) Peak cell density (cells/cm^3): 1.00000E+38 (=KAP) Tissue volume (cm^3): 1.00000E+00 (=VOL) Maximum number of cells (cells): 1.00000E+38 (=KAP*VOL) -------------------------------------------------------------------------------- RMRM TABLE: Summary of RMR parameters and related (derived) quantities. ======================================================================== G0/G1 DNA CONTENT: 5667.000 Mbp/cell = 3.482938E+12 Da/cell ENDOGENOUS DAMAGE: per (h-Da) per (h-Mbp) per (h-cell) DSB = 0.00000E+00 0.00000E+00 0.00000E+00 DAMAGE FORMATION: per (Gy-Da) per (Gy-Mbp) per (Gy-cell) DSB = 1.14846E-11 7.05841E-03 40.000000 DAMAGE REPAIR/MISREPAIR: A0= 0.000000E+00 (=0.000%) (1-A0)*PHI= 5.543334E-03 (=0.554%) (1-A0)*(1-PHI)= 9.944567E-01 (=99.446%) RHT= 2.000000 h (=120.00 minute) LAM= 3.4657359E-01 (1/h) BMR= 1.4836442E-04 (1/h) LAM/BMR= 2.3359616E+03 GAM= 0.250 ***** EQUIVALENT LQ MODEL PARAMETERS ***** alpha = 0.221733 (1/Gy) beta = 0.083719 (1/Gy^2) alpha/beta = 2.648530 (Gy) ------------------------------------------------------------------------ NOTE: 1 bp = 614.6 Da (amu) = 1.0205E-09 pg MCAT FIT: CELL SURVIVAL AS A FUNCTION OF DOSE AND DOSE RATE (PC3B cells). ===================================================================================================== -------- EXPOSURE SUMMARY -------- Duration Dose Dose Rate ---------------- SURVIVING FRACTION -------------- EXPERIMENT (h) (Gy) (Gy/h)* Measured Norm. Wt Predicted Residual ----------------------------------------------------------------------------------------------------- 1 2.7778E-13 0.0000E+00 0.0000E+00 9.99941E-01 1.43E-01 1.00000E+00 5.9000E-05 2 1.1893E-02 9.9900E-01 8.4000E+01 7.82405E-01 1.43E-01 7.37853E-01 -6.0380E-02 3 2.3821E-02 2.0010E+00 8.4000E+01 4.55533E-01 1.43E-01 4.62761E-01 1.5619E-02 4 3.5702E-02 2.9990E+00 8.4000E+01 2.28105E-01 1.43E-01 2.49030E-01 8.4027E-02 5 4.7631E-02 4.0010E+00 8.4000E+01 1.17650E-01 1.43E-01 1.15068E-01 -2.2439E-02 6 5.9595E-02 5.0060E+00 8.4000E+01 4.86160E-02 1.43E-01 4.58632E-02 -6.0021E-02 7 7.1524E-02 6.0080E+00 8.4000E+01 1.53510E-02 1.43E-01 1.59367E-02 3.6752E-02 ----------------------------------------------------------------------------------------------------- RESIDUAL: Stats: min=-6.038E-02 max=8.403E-02, mean=-9.1203E-04, stdev=5.2283E-02, var=2.7335E-03 Student's T test (mean=0, NDF=4): Pr>ABS(0.04615)= 9.6540E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.94565)=7.062E-01 (passed normality test) WEIGHTED RESIDUAL: Stats: min=-2.282E-02 max=3.176E-02, mean=-3.4472E-04, stdev=1.9761E-02, var=3.9050E-04 Student's T test (mean=0, NDF=4): Pr>ABS(0.04615)= 9.6540E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.94565)=7.062E-01 (passed normality test) NOX=7, NPAR=2 CFE.SF: Cell survival data for PC3B cells. ========================================================================================= -------- EXPOSURE SUMMARY -------- ------------ ENDPOINT ------------- DATASET ID Duration Dose Dose Rate Fraction Fraction Standard CFE RADX (h) (Gy) (Gy/h) Killed Viable Error (%) ----------------------------------------------------------------------------------------- 1 1 2.7778E-13 0.0000E+00 0.0000E+00 5.90000E-05 9.99941E-01 1.00E+01 2 2 1.1893E-02 9.9900E-01 8.4000E+01 2.17595E-01 7.82405E-01 1.00E+01 3 3 2.3821E-02 2.0010E+00 8.4000E+01 5.44467E-01 4.55533E-01 1.00E+01 4 4 3.5702E-02 2.9990E+00 8.4000E+01 7.71895E-01 2.28105E-01 1.00E+01 5 5 4.7631E-02 4.0010E+00 8.4000E+01 8.82350E-01 1.17650E-01 1.00E+01 6 6 5.9595E-02 5.0060E+00 8.4000E+01 9.51384E-01 4.86160E-02 1.00E+01 7 7 7.1524E-02 6.0080E+00 8.4000E+01 9.84649E-01 1.53510E-02 1.00E+01 ----------------------------------------------------------------------------------------- CFE.DXA: Additional details from the cell survival assay (PC3B). ===================================================================================== DATASET ID ---------- TIME (h) ----------- DOSE ---- LETHAL DAMAGE --- CFE RADX RADX TTP ETSI (Gy) #/(cell Gy) #/(cell) ------------------------------------------------------------------------------------- 1 1 2.778E-13 1.200E+01 4.000E+02 0.0000E+00 0.0000E+00 0.0000E+00 2 2 1.189E-02 1.200E+01 4.000E+02 9.9900E-01 2.4563E-01 2.4538E-01 3 3 2.382E-02 1.200E+01 4.000E+02 2.0010E+00 3.9295E-01 7.8629E-01 4 4 3.570E-02 1.200E+01 4.000E+02 2.9990E+00 4.9281E-01 1.4779E+00 5 5 4.763E-02 1.200E+01 4.000E+02 4.0010E+00 5.3488E-01 2.1400E+00 6 6 5.960E-02 1.200E+01 4.000E+02 5.0060E+00 6.0404E-01 3.0238E+00 7 7 7.152E-02 1.200E+01 4.000E+02 6.0080E+00 6.9517E-01 4.1766E+00 ------------------------------------------------------------------------------------- ETSI: Total elapsed time (after irradiation) before colonies are counted. TTP: Time available for repair before cells are plated. Total VC execution time (min): 2.34333E-02 VC 2.00J 25-SEP-2003 19-NOV-2003 10:29:59 AM