VC 2.00J 25-SEP-2003 11-NOV-2003 09:48:51 AM by RD Stewart (trebor@purdue.edu) PROGRAM FILE INPUT/OUTPUT AND RUN-TIME OPTIONS: ================================================================================ CMD: vc rmr.inp ..\xdat\pc3.xdat -c FNI: rmr.inp FNO: rmr.out RADX: ..\xdat\pc3.radx (8 exposures) XDAT: ..\xdat\pc3.xdat (8 experiments) -------------------------------------------------------------------------------- MCAT SUMMARY: BEST-FIT MODEL PARAMETERS (method = BCONF): ========================================================================================== --- PARAMETER --- ----- CONSTRAINTS ----- INITIAL OPTIMAL PID CID NAM Minimum Maximum GUESS VALUE 2001 CLRP BMR 0.00000E+00 1.00000E+00 1.95000E-05 1.9710144E-05 2401 CLRP PHI 0.00000E+00 1.00000E+00 3.62500E-03 3.4228370E-03 2201 CLRP RHT 1.00000E-02 1.20000E+01 6.50000E+00 6.7346443E+00 ------------------------------------------------------------------------------------------ FIGURE OF MERIT (FOM): 1.23319E-02 (nfom=25921, nsim=207368, nmx=8, nox=8, ndf=4, nap=3) COMBINED UNWEIGHTED RESIDUAL: Stats: min=-1.394E-01 max=1.519E-01, mean=-2.3856E-03, stdev=1.0544E-01, var=1.1117E-02 Student's T test (mean=0, NDF=4): Pr>ABS(0.06400)= 9.5204E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.94560)=6.699E-01 (passed normality test) COMBINED WEIGHTED RESIDUAL: Stats: min=-4.927E-02 max=5.371E-02, mean=-8.4344E-04, stdev=3.7277E-02, var=1.3896E-03 Student's T test (mean=0, NDF=4): Pr>ABS(0.06400)= 9.5204E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.94560)=6.699E-01 (passed normality test) QECK TABLE: Cell and tissue kinetic parameters. ================================================================================ Duration of cell cycle (TCC): 10.00000 h Potential doubling time (Tpot): 480.00000 h (= 20.000 days) Cell birth rate (AX): 1.6636E+00 cell/day (= LOG(2)/TCC) Cell death rate (BX): 1.6289E+00 cell/day [=AX-LOG(2)/TPOT] Net cell birth rate (AX-BX): 3.4657E-02 cell/day Initial Number of Cells: non-dividing: 1.00000E+00 (100.000% = 1-GF) dividing: 0.00000E+00 ( 0.000% = GF) total: 1.00000E+00 (100.000% total) Life Support Capacity of System: Initial system capacity utilization: 0.000000% (=F0) Peak cell density (cells/cm^3): 1.00000E+38 (=KAP) Tissue volume (cm^3): 1.00000E+00 (=VOL) Maximum number of cells (cells): 1.00000E+38 (=KAP*VOL) -------------------------------------------------------------------------------- RMRM TABLE: Summary of RMR parameters and related (derived) quantities. ======================================================================== G0/G1 DNA CONTENT: 5667.000 Mbp/cell = 3.482938E+12 Da/cell ENDOGENOUS DAMAGE: per (h-Da) per (h-Mbp) per (h-cell) DSB = 0.00000E+00 0.00000E+00 0.00000E+00 DAMAGE FORMATION: per (Gy-Da) per (Gy-Mbp) per (Gy-cell) DSB = 1.14846E-11 7.05841E-03 40.000000 DAMAGE REPAIR/MISREPAIR: A0= 0.000000E+00 (=0.000%) (1-A0)*PHI= 3.422837E-03 (=0.342%) (1-A0)*(1-PHI)= 9.965772E-01 (=99.658%) RHT= 6.734644 h (=404.08 minute) LAM= 1.0292261E-01 (1/h) BMR= 1.9710144E-05 (1/h) LAM/BMR= 5.2218091E+03 GAM= 0.250 ***** EQUIVALENT LQ MODEL PARAMETERS ***** alpha = 0.136913 (1/Gy) beta = 0.037777 (1/Gy^2) alpha/beta = 3.624302 (Gy) ------------------------------------------------------------------------ NOTE: 1 bp = 614.6 Da (amu) = 1.0205E-09 pg MCAT FIT: CELL SURVIVAL AS A FUNCTION OF DOSE AND DOSE RATE (PC3 cells). ===================================================================================================== -------- EXPOSURE SUMMARY -------- Duration Dose Dose Rate ---------------- SURVIVING FRACTION -------------- EXPERIMENT (h) (Gy) (Gy/h)* Measured Norm. Wt Predicted Residual ----------------------------------------------------------------------------------------------------- 1 2.4000E+01 6.0000E+00 2.5000E-01 2.08547E-01 1.25E-01 2.21975E-01 6.0495E-02 2 4.7752E+01 1.1938E+01 2.5000E-01 3.13760E-02 1.25E-01 3.54370E-02 1.1460E-01 3 7.1572E+01 1.7893E+01 2.5000E-01 5.80823E-03 1.25E-01 5.47814E-03 -6.0256E-02 4 3.3588E-02 2.0153E+00 6.0000E+01 7.15134E-01 1.25E-01 6.49434E-01 -1.0117E-01 5 6.6543E-02 3.9926E+00 6.0000E+01 3.63164E-01 1.25E-01 3.18743E-01 -1.3936E-01 6 9.9663E-02 5.9798E+00 6.0000E+01 1.15640E-01 1.25E-01 1.18027E-01 2.0225E-02 7 1.3269E-01 7.9612E+00 6.0000E+01 2.84121E-02 1.25E-01 3.35016E-02 1.5192E-01 8 1.6589E-01 9.9535E+00 6.0000E+01 7.74348E-03 1.25E-01 7.26722E-03 -6.5536E-02 ----------------------------------------------------------------------------------------------------- RESIDUAL: Stats: min=-1.394E-01 max=1.519E-01, mean=-2.3856E-03, stdev=1.0544E-01, var=1.1117E-02 Student's T test (mean=0, NDF=4): Pr>ABS(0.06400)= 9.5204E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.94560)=6.699E-01 (passed normality test) WEIGHTED RESIDUAL: Stats: min=-4.927E-02 max=5.371E-02, mean=-8.4344E-04, stdev=3.7277E-02, var=1.3896E-03 Student's T test (mean=0, NDF=4): Pr>ABS(0.06400)= 9.5204E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.94560)=6.699E-01 (passed normality test) NOX=8, NPAR=3 CFE.SF: Cell survival data for PC3 cells. ========================================================================================= -------- EXPOSURE SUMMARY -------- ------------ ENDPOINT ------------- DATASET ID Duration Dose Dose Rate Fraction Fraction Standard CFE RADX (h) (Gy) (Gy/h) Killed Viable Error (%) ----------------------------------------------------------------------------------------- 1 1 2.4000E+01 6.0000E+00 2.5000E-01 7.91453E-01 2.08547E-01 1.00E+01 2 2 4.7752E+01 1.1938E+01 2.5000E-01 9.68624E-01 3.13760E-02 1.00E+01 3 3 7.1572E+01 1.7893E+01 2.5000E-01 9.94192E-01 5.80823E-03 1.00E+01 4 4 3.3588E-02 2.0153E+00 6.0000E+01 2.84866E-01 7.15134E-01 1.00E+01 5 5 6.6543E-02 3.9926E+00 6.0000E+01 6.36836E-01 3.63164E-01 1.00E+01 6 6 9.9663E-02 5.9798E+00 6.0000E+01 8.84360E-01 1.15640E-01 1.00E+01 7 7 1.3269E-01 7.9612E+00 6.0000E+01 9.71588E-01 2.84121E-02 1.00E+01 8 8 1.6589E-01 9.9535E+00 6.0000E+01 9.92257E-01 7.74348E-03 1.00E+01 ----------------------------------------------------------------------------------------- CFE.DXA: Additional details from the cell survival assay (PC3). ===================================================================================== DATASET ID ---------- TIME (h) ----------- DOSE ---- LETHAL DAMAGE --- CFE RADX RADX TTP ETSI (Gy) #/(cell Gy) #/(cell) ------------------------------------------------------------------------------------- 1 1 2.400E+01 1.200E+01 4.000E+02 6.0000E+00 2.6127E-01 1.5676E+00 2 2 4.775E+01 1.200E+01 4.000E+02 1.1938E+01 2.8997E-01 3.4617E+00 3 3 7.157E+01 1.200E+01 4.000E+02 1.7893E+01 2.8774E-01 5.1485E+00 4 4 3.359E-02 1.200E+01 4.000E+02 2.0153E+00 1.6637E-01 3.3529E-01 5 5 6.654E-02 1.200E+01 4.000E+02 3.9926E+00 2.5369E-01 1.0129E+00 6 6 9.966E-02 1.200E+01 4.000E+02 5.9798E+00 3.6076E-01 2.1573E+00 7 7 1.327E-01 1.200E+01 4.000E+02 7.9612E+00 4.4729E-01 3.5609E+00 8 8 1.659E-01 1.200E+01 4.000E+02 9.9535E+00 4.8836E-01 4.8609E+00 ------------------------------------------------------------------------------------- ETSI: Total elapsed time (after irradiation) before colonies are counted. TTP: Time available for repair before cells are plated. Total VC execution time (min): 11.259 VC 2.00J 25-SEP-2003 11-NOV-2003 10:00:06 AM