VC 2.00J 25-SEP-2003 20-NOV-2003 09:59:45 PM by RD Stewart (trebor@purdue.edu) PROGRAM FILE INPUT/OUTPUT AND RUN-TIME OPTIONS: ================================================================================ CMD: vc lpl.inp ..\xdat\du145.xdat -c FNI: lpl.inp FNO: lpl.out RADX: ..\xdat\du145.radx (6 exposures) XDAT: ..\xdat\du145.xdat (6 experiments) -------------------------------------------------------------------------------- MCAT SUMMARY: BEST-FIT MODEL PARAMETERS (method = BCONF): ========================================================================================== --- PARAMETER --- ----- CONSTRAINTS ----- INITIAL OPTIMAL PID CID NAM Minimum Maximum GUESS VALUE 2001 CLRP BMR 0.00000E+00 1.00000E+00 1.00000E-04 3.1324991E-05 1101 IRAD FL 0.00000E+00 2.00000E+00 1.00000E-01 1.5575049E-01 ------------------------------------------------------------------------------------------ FIGURE OF MERIT (FOM): 3.42408E-03 (nfom=290, nsim=1740, nmx=6, nox=6, ndf=3, nap=2) COMBINED UNWEIGHTED RESIDUAL: Stats: min=-3.423E-02 max=2.806E-02, mean=1.6789E-04, stdev=2.2505E-02, var=5.0646E-04 Student's T test (mean=0, NDF=3): Pr>ABS(0.01827)= 9.8657E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.96673)=8.690E-01 (passed normality test) COMBINED WEIGHTED RESIDUAL: Stats: min=-1.398E-02 max=1.145E-02, mean=6.8540E-05, stdev=9.1875E-03, var=8.4410E-05 Student's T test (mean=0, NDF=3): Pr>ABS(0.01827)= 9.8657E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.96673)=8.690E-01 (passed normality test) QECK TABLE: Cell and tissue kinetic parameters. ================================================================================ Duration of cell cycle (TCC): 10.00000 h Potential doubling time (Tpot): 480.00000 h (= 20.000 days) Cell birth rate (AX): 1.6636E+00 cell/day (= LOG(2)/TCC) Cell death rate (BX): 1.6289E+00 cell/day [=AX-LOG(2)/TPOT] Net cell birth rate (AX-BX): 3.4657E-02 cell/day Initial Number of Cells: non-dividing: 1.00000E+00 (100.000% = 1-GF) dividing: 0.00000E+00 ( 0.000% = GF) total: 1.00000E+00 (100.000% total) Life Support Capacity of System: Initial system capacity utilization: 0.000000% (=F0) Peak cell density (cells/cm^3): 1.00000E+38 (=KAP) Tissue volume (cm^3): 1.00000E+00 (=VOL) Maximum number of cells (cells): 1.00000E+38 (=KAP*VOL) -------------------------------------------------------------------------------- LPLM TABLE: Summary of LPL parameters and related (derived) quantities. ======================================================================== G0/G1 DNA CONTENT: 5667.000 Mbp/cell = 3.482938E+12 Da/cell ENDOGENOUS DAMAGE: per (h-Da) per (h-Mbp) per (h-cell) DSB = 0.00000E+00 0.00000E+00 0.00000E+00 RADIATION DAMAGE: per (Gy-Da) per (Gy-Mbp) per (Gy-cell) DSB = 1.14846E-11 7.05841E-03 40.000000 FL = 4.47181E-14 2.74838E-05 0.155750 DAMAGE REPAIR/MISREPAIR: A0= 9.500000E-01 (=95.000%) (1-A0)= 5.000000E-02 (=5.000%) RHT= 6.000000 h (=360.00 minute) LAM= 1.1552453E-01 (1/h) BMR= 3.1324991E-05 (1/h) LAM/BMR= 3.6879350E+03 GAM= 0.250 ***** EQUIVALENT LQ MODEL PARAMETERS ***** alpha = 0.155750 (1/Gy) beta = 0.054231 (1/Gy^2) alpha/beta = 2.871988 (Gy) ------------------------------------------------------------------------ NOTE: 1 bp = 614.6 Da (amu) = 1.0205E-09 pg MCAT FIT: CELL SURVIVAL AS A FUNCTION OF DOSE AND DOSE RATE (DU145 cells). ===================================================================================================== -------- EXPOSURE SUMMARY -------- Duration Dose Dose Rate ---------------- SURVIVING FRACTION -------------- EXPERIMENT (h) (Gy) (Gy/h)* Measured Norm. Wt Predicted Residual ----------------------------------------------------------------------------------------------------- 1 1.5717E-02 9.4300E-01 6.0000E+01 8.20989E-01 1.67E-01 8.22578E-01 1.9322E-03 2 3.3133E-02 1.9880E+00 6.0000E+01 5.88664E-01 1.67E-01 5.93424E-01 8.0210E-03 3 4.9717E-02 2.9830E+00 6.0000E+01 4.04862E-01 1.67E-01 3.91461E-01 -3.4232E-02 4 6.6167E-02 3.9700E+00 6.0000E+01 2.31292E-01 1.67E-01 2.34684E-01 1.4451E-02 5 8.3417E-02 5.0050E+00 6.0000E+01 1.20274E-01 1.67E-01 1.23746E-01 2.8056E-02 6 9.9950E-02 5.9970E+00 6.0000E+01 6.18490E-02 1.67E-01 6.08019E-02 -1.7221E-02 ----------------------------------------------------------------------------------------------------- RESIDUAL: Stats: min=-3.423E-02 max=2.806E-02, mean=1.6789E-04, stdev=2.2505E-02, var=5.0646E-04 Student's T test (mean=0, NDF=3): Pr>ABS(0.01827)= 9.8657E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.96673)=8.690E-01 (passed normality test) WEIGHTED RESIDUAL: Stats: min=-1.398E-02 max=1.145E-02, mean=6.8540E-05, stdev=9.1875E-03, var=8.4410E-05 Student's T test (mean=0, NDF=3): Pr>ABS(0.01827)= 9.8657E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.96673)=8.690E-01 (passed normality test) NOX=6, NPAR=2 CFE.SF: Cell survival data for DU145 cells. ========================================================================================= -------- EXPOSURE SUMMARY -------- ------------ ENDPOINT ------------- DATASET ID Duration Dose Dose Rate Fraction Fraction Standard CFE RADX (h) (Gy) (Gy/h) Killed Viable Error (%) ----------------------------------------------------------------------------------------- 1 1 1.5717E-02 9.4300E-01 6.0000E+01 1.79011E-01 8.20989E-01 1.00E+01 2 2 3.3133E-02 1.9880E+00 6.0000E+01 4.11336E-01 5.88664E-01 1.00E+01 3 3 4.9717E-02 2.9830E+00 6.0000E+01 5.95138E-01 4.04862E-01 1.00E+01 4 4 6.6167E-02 3.9700E+00 6.0000E+01 7.68708E-01 2.31292E-01 1.00E+01 5 5 8.3417E-02 5.0050E+00 6.0000E+01 8.79726E-01 1.20274E-01 1.00E+01 6 6 9.9950E-02 5.9970E+00 6.0000E+01 9.38151E-01 6.18490E-02 1.00E+01 ----------------------------------------------------------------------------------------- CFE.DXA: Additional details from the cell survival assay (DU145). ===================================================================================== DATASET ID ---------- TIME (h) ----------- DOSE ---- LETHAL DAMAGE --- CFE RADX RADX TTP ETSI (Gy) #/(cell Gy) #/(cell) ------------------------------------------------------------------------------------- 1 1 1.572E-02 1.200E+01 4.000E+02 9.4300E-01 2.0917E-01 1.9725E-01 2 2 3.313E-02 1.200E+01 4.000E+02 1.9880E+00 2.6655E-01 5.2990E-01 3 3 4.972E-02 1.200E+01 4.000E+02 2.9830E+00 3.0312E-01 9.0421E-01 4 4 6.617E-02 1.200E+01 4.000E+02 3.9700E+00 3.6878E-01 1.4641E+00 5 5 8.342E-02 1.200E+01 4.000E+02 5.0050E+00 4.2317E-01 2.1180E+00 6 6 9.995E-02 1.200E+01 4.000E+02 5.9970E+00 4.6408E-01 2.7831E+00 ------------------------------------------------------------------------------------- ETSI: Total elapsed time (after irradiation) before colonies are counted. TTP: Time available for repair before cells are plated. Total VC execution time (min): 9.23667E-02 VC 2.00J 25-SEP-2003 20-NOV-2003 09:59:50 PM