VC 2.00J 25-SEP-2003 20-NOV-2003 06:22:58 PM by RD Stewart (trebor@purdue.edu) PROGRAM FILE INPUT/OUTPUT AND RUN-TIME OPTIONS: ================================================================================ CMD: vc rmr.inp ..\xdat\du145.xdat -c FNI: rmr.inp FNO: rmr.out RADX: ..\xdat\du145.radx (8 exposures) XDAT: ..\xdat\du145.xdat (8 experiments) -------------------------------------------------------------------------------- MCAT SUMMARY: BEST-FIT MODEL PARAMETERS (method = BCONF): ========================================================================================== --- PARAMETER --- ----- CONSTRAINTS ----- INITIAL OPTIMAL PID CID NAM Minimum Maximum GUESS VALUE 2001 CLRP BMR 0.00000E+00 1.00000E+00 1.95000E-05 6.8767381E-05 2401 CLRP PHI 0.00000E+00 1.00000E+00 3.62500E-03 7.5704226E-03 2201 CLRP RHT 1.00000E-02 1.20000E+01 2.00000E+00 5.8320194E+00 ------------------------------------------------------------------------------------------ FIGURE OF MERIT (FOM): 1.01794E-02 (nfom=1510, nsim=12080, nmx=8, nox=8, ndf=4, nap=3) COMBINED UNWEIGHTED RESIDUAL: Stats: min=-1.024E-01 max=1.114E-01, mean=1.0089E-02, stdev=8.6387E-02, var=7.4627E-03 Student's T test (mean=0, NDF=4): Pr>ABS(0.33032)= 7.5773E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.87477)=1.708E-01 (passed normality test) COMBINED WEIGHTED RESIDUAL: Stats: min=-3.619E-02 max=3.939E-02, mean=3.5670E-03, stdev=3.0542E-02, var=9.3284E-04 Student's T test (mean=0, NDF=4): Pr>ABS(0.33032)= 7.5773E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.87477)=1.708E-01 (passed normality test) QECK TABLE: Cell and tissue kinetic parameters. ================================================================================ Duration of cell cycle (TCC): 10.00000 h Potential doubling time (Tpot): 480.00000 h (= 20.000 days) Cell birth rate (AX): 1.6636E+00 cell/day (= LOG(2)/TCC) Cell death rate (BX): 1.6289E+00 cell/day [=AX-LOG(2)/TPOT] Net cell birth rate (AX-BX): 3.4657E-02 cell/day Initial Number of Cells: non-dividing: 1.00000E+00 (100.000% = 1-GF) dividing: 0.00000E+00 ( 0.000% = GF) total: 1.00000E+00 (100.000% total) Life Support Capacity of System: Initial system capacity utilization: 0.000000% (=F0) Peak cell density (cells/cm^3): 1.00000E+38 (=KAP) Tissue volume (cm^3): 1.00000E+00 (=VOL) Maximum number of cells (cells): 1.00000E+38 (=KAP*VOL) -------------------------------------------------------------------------------- RMRM TABLE: Summary of RMR parameters and related (derived) quantities. ======================================================================== G0/G1 DNA CONTENT: 5667.000 Mbp/cell = 3.482938E+12 Da/cell ENDOGENOUS DAMAGE: per (h-Da) per (h-Mbp) per (h-cell) DSB = 0.00000E+00 0.00000E+00 0.00000E+00 DAMAGE FORMATION: per (Gy-Da) per (Gy-Mbp) per (Gy-cell) DSB = 5.74228E-12 3.52920E-03 20.000000 DAMAGE REPAIR/MISREPAIR: A0= 0.000000E+00 (=0.000%) (1-A0)*PHI= 7.570423E-03 (=0.757%) (1-A0)*(1-PHI)= 9.924296E-01 (=99.243%) RHT= 5.832019 h (=349.92 minute) LAM= 1.1885200E-01 (1/h) BMR= 6.8767381E-05 (1/h) LAM/BMR= 1.7283194E+03 GAM= 0.250 ***** EQUIVALENT LQ MODEL PARAMETERS ***** alpha = 0.151408 (1/Gy) beta = 0.028054 (1/Gy^2) alpha/beta = 5.397076 (Gy) ------------------------------------------------------------------------ NOTE: 1 bp = 614.6 Da (amu) = 1.0205E-09 pg MCAT FIT: CELL SURVIVAL AS A FUNCTION OF DOSE AND DOSE RATE (DU145 cells). ===================================================================================================== -------- EXPOSURE SUMMARY -------- Duration Dose Dose Rate ---------------- SURVIVING FRACTION -------------- EXPERIMENT (h) (Gy) (Gy/h)* Measured Norm. Wt Predicted Residual ----------------------------------------------------------------------------------------------------- 1 2.3996E+01 5.9990E+00 2.5000E-01 2.80237E-01 1.25E-01 2.54217E-01 -1.0235E-01 2 4.7784E+01 1.1946E+01 2.5000E-01 5.75430E-02 1.25E-01 5.34582E-02 -7.6412E-02 3 7.1640E+01 1.7910E+01 2.5000E-01 1.02730E-02 1.25E-01 1.10773E-02 7.2610E-02 4 3.3583E-02 2.0150E+00 6.0000E+01 6.15280E-01 1.25E-01 6.58667E-01 6.5871E-02 5 6.6628E-02 3.9977E+00 6.0000E+01 3.14080E-01 1.25E-01 3.53464E-01 1.1142E-01 6 9.9763E-02 5.9858E+00 6.0000E+01 1.61160E-01 1.25E-01 1.54919E-01 -4.0284E-02 7 1.3279E-01 7.9676E+00 6.0000E+01 5.05200E-02 1.25E-01 5.62516E-02 1.0189E-01 8 1.6592E-01 9.9550E+00 6.0000E+01 1.78500E-02 1.25E-01 1.69671E-02 -5.2033E-02 ----------------------------------------------------------------------------------------------------- RESIDUAL: Stats: min=-1.024E-01 max=1.114E-01, mean=1.0089E-02, stdev=8.6387E-02, var=7.4627E-03 Student's T test (mean=0, NDF=4): Pr>ABS(0.33032)= 7.5773E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.87477)=1.708E-01 (passed normality test) WEIGHTED RESIDUAL: Stats: min=-3.619E-02 max=3.939E-02, mean=3.5670E-03, stdev=3.0542E-02, var=9.3284E-04 Student's T test (mean=0, NDF=4): Pr>ABS(0.33032)= 7.5773E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.87477)=1.708E-01 (passed normality test) NOX=8, NPAR=3 CFE.SF: Cell survival data for DU145 cells. ========================================================================================= -------- EXPOSURE SUMMARY -------- ------------ ENDPOINT ------------- DATASET ID Duration Dose Dose Rate Fraction Fraction Standard CFE RADX (h) (Gy) (Gy/h) Killed Viable Error (%) ----------------------------------------------------------------------------------------- 1 1 2.3996E+01 5.9990E+00 2.5000E-01 7.19763E-01 2.80237E-01 1.00E+01 2 2 4.7784E+01 1.1946E+01 2.5000E-01 9.42457E-01 5.75430E-02 1.00E+01 3 3 7.1640E+01 1.7910E+01 2.5000E-01 9.89727E-01 1.02730E-02 1.00E+01 4 4 3.3583E-02 2.0150E+00 6.0000E+01 3.84720E-01 6.15280E-01 1.00E+01 5 5 6.6628E-02 3.9977E+00 6.0000E+01 6.85920E-01 3.14080E-01 1.00E+01 6 6 9.9763E-02 5.9858E+00 6.0000E+01 8.38840E-01 1.61160E-01 1.00E+01 7 7 1.3279E-01 7.9676E+00 6.0000E+01 9.49480E-01 5.05200E-02 1.00E+01 8 8 1.6592E-01 9.9550E+00 6.0000E+01 9.82150E-01 1.78500E-02 1.00E+01 ----------------------------------------------------------------------------------------- CFE.DXA: Additional details from the cell survival assay (DU145). ===================================================================================== DATASET ID ---------- TIME (h) ----------- DOSE ---- LETHAL DAMAGE --- CFE RADX RADX TTP ETSI (Gy) #/(cell Gy) #/(cell) ------------------------------------------------------------------------------------- 1 1 2.400E+01 1.200E+01 4.000E+02 5.9990E+00 2.1206E-01 1.2721E+00 2 2 4.778E+01 1.200E+01 4.000E+02 1.1946E+01 2.3901E-01 2.8552E+00 3 3 7.164E+01 1.200E+01 4.000E+02 1.7910E+01 2.5562E-01 4.5782E+00 4 4 3.358E-02 1.200E+01 4.000E+02 2.0150E+00 2.4103E-01 4.8568E-01 5 5 6.663E-02 1.200E+01 4.000E+02 3.9977E+00 2.8969E-01 1.1581E+00 6 6 9.976E-02 1.200E+01 4.000E+02 5.9858E+00 3.0495E-01 1.8254E+00 7 7 1.328E-01 1.200E+01 4.000E+02 7.9676E+00 3.7469E-01 2.9854E+00 8 8 1.659E-01 1.200E+01 4.000E+02 9.9550E+00 4.0439E-01 4.0258E+00 ------------------------------------------------------------------------------------- ETSI: Total elapsed time (after irradiation) before colonies are counted. TTP: Time available for repair before cells are plated. Total VC execution time (min): 0.63990 VC 2.00J 25-SEP-2003 20-NOV-2003 06:23:37 PM