VC 2.00I 15-JUL-2003 01-AUG-2003 01:46:12 PM by RD Stewart (trebor@purdue.edu) PROGRAM FILE INPUT/OUTPUT AND RUN-TIME OPTIONS: ================================================================================ CMD: vc tlk.inp ..\xdat\a549.xdat -c FNI: tlk.inp FNO: tlk.out RADX: ..\xdat\a549.radx (11 exposures) XDAT: ..\xdat\a549.xdat (11 experiments) -------------------------------------------------------------------------------- MCAT SUMMARY: BEST-FIT MODEL PARAMETERS (method = BCONF): ========================================================================================== --- PARAMETER --- ----- CONSTRAINTS ----- INITIAL OPTIMAL PID CID NAM Minimum Maximum GUESS VALUE 2001 CLRP BMR 0.00000E+00 1.00000E-01 2.00000E-04 1.1528046E-03 2202 CLRP RHT 1.00000E-02 1.20000E+01 8.00000E+00 2.3618067E+00 ------------------------------------------------------------------------------------------ FIGURE OF MERIT (FOM): 3.03726E-02 (nfom=110, nsim=1210, nmx=11, nox=11, ndf=8, nap=2) COMBINED UNWEIGHTED RESIDUAL: Stats: min=-4.951E-01 max=6.391E-01, mean=1.0918E-01, stdev=3.3117E-01, var=1.0967E-01 Student's T test (mean=0, NDF=8): Pr>ABS(1.09344)= 3.0603E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.96933)=8.650E-01 (passed normality test) COMBINED WEIGHTED RESIDUAL: Stats: min=-1.493E-01 max=1.927E-01, mean=3.2919E-02, stdev=9.9851E-02, var=9.9701E-03 Student's T test (mean=0, NDF=8): Pr>ABS(1.09344)= 3.0603E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.96933)=8.650E-01 (passed normality test) QECK TABLE: Cell and tissue kinetic parameters. ================================================================================ Duration of cell cycle (TCC): 10.00000 h Potential doubling time (Tpot): 480.00000 h (= 20.000 days) Cell birth rate (AX): 1.6636E+00 cell/day (= LOG(2)/TCC) Cell death rate (BX): 1.6289E+00 cell/day [=AX-LOG(2)/TPOT] Net cell birth rate (AX-BX): 3.4657E-02 cell/day Initial Number of Cells: non-dividing: 1.00000E+00 (100.000% = 1-GF) dividing: 0.00000E+00 ( 0.000% = GF) total: 1.00000E+00 (100.000% total) Life Support Capacity of System: Initial system capacity utilization: 0.000000% (=F0) Peak cell density (cells/cm^3): 1.00000E+38 (=KAP) Tissue volume (cm^3): 1.00000E+00 (=VOL) Maximum number of cells (cells): 1.00000E+38 (=KAP*VOL) -------------------------------------------------------------------------------- TLKM TABLE: Summary of TLK parameters and related (derived) quantities. ======================================================================== G0/G1 DNA CONTENT: 5667.000 Mbp/cell = 3.482938E+12 Da/cell ENDOGENOUS DAMAGE: per (h-Da) per (h-Mbp) per (h-cell) Simple DSB = 0.00000E+00 0.00000E+00 0.00000E+00 Complex DSB = 0.00000E+00 0.00000E+00 0.00000E+00 Total DSB = 0.00000E+00 0.00000E+00 0.00000E+00 DAMAGE FORMATION: per (Gy-Da) per (Gy-Mbp) per (Gy-cell) Simple DSB = 5.74228E-12 3.52920E-03 20.000000 Complex DSB = 1.43557E-12 8.82301E-04 5.000000 Total DSB = 7.17785E-12 4.41151E-03 25.000000 DAMAGE REPAIR/MISREPAIR: A1= 7.500000E-01 (=75.000%) A2= 2.500000E-01 (=25.000%) PHI1= 1.000000E-02 (=1.000%) PHI2= 1.000000E-02 (=1.000%) (1-A1)*PHI1= 2.500000E-03 (=0.250%) (1-A2)*PHI2= 7.500000E-03 (=0.750%) (1-A1)*(1-PHI1)= 2.475000E-01 (=24.750%) (1-A2)*(1-PHI2)= 7.425000E-01 (=74.250%) RHT1= 0.250000 h (=15.00 minute) RHT2= 2.361807 h (=141.71 minute) LAM1= 2.7725887E+00 (1/h) LAM2= 2.9348175E-01 (1/h) BMR= 1.1528046E-03 (1/h) LAM1/BMR= 2.4050812E+03 LAM2/BMR= 2.5458066E+02 (LAM1+LAM2)/BMR= 2.6596619E+03 GAM= 0.250 ************** EQUIVALENT LQ MODEL PARAMETERS ************************** Simple Complex Total alpha: 5.0000E-02 + 3.7500E-02 = 8.7500000E-02 (1/Gy) beta: 2.9793E-02 + 2.1119E-02 = 5.0911655E-02 (1/Gy^2) alpha/beta: 1.7186634 (Gy) ------------------------------------------------------------------------ NOTE: 1 bp = 614.6 Da (amu) = 1.0205E-09 pg MCAT FIT: CELL SURVIVAL AS A FUNCTION OF DOSE AND DOSE RATE (A 549 cells). ===================================================================================================== -------- EXPOSURE SUMMARY -------- Duration Dose Dose Rate ---------------- SURVIVING FRACTION -------------- EXPERIMENT (h) (Gy) (Gy/h)* Measured Norm. Wt Predicted Residual ----------------------------------------------------------------------------------------------------- 1 3.8933E+00 2.3360E+00 6.0000E-01 6.89071E-01 9.09E-02 7.32987E-01 5.9914E-02 2 1.2255E+01 7.3530E+00 6.0000E-01 3.70723E-01 9.09E-02 3.10183E-01 -1.9517E-01 3 2.0118E+01 1.2071E+01 6.0000E-01 1.99559E-01 9.09E-02 1.33472E-01 -4.9514E-01 4 2.7985E+01 1.6791E+01 6.0000E-01 2.33280E-02 9.09E-02 5.72709E-02 5.9267E-01 5 3.5705E+01 2.1423E+01 6.0000E-01 9.00800E-03 9.09E-02 2.49601E-02 6.3910E-01 6 1.2233E-02 1.4680E+00 1.2000E+02 7.55016E-01 9.09E-02 7.90331E-01 4.4684E-02 7 2.4817E-02 2.9780E+00 1.2000E+02 3.73661E-01 9.09E-02 5.02170E-01 2.5591E-01 8 3.7650E-02 4.5180E+00 1.2000E+02 2.13499E-01 9.09E-02 2.57839E-01 1.7197E-01 9 5.1542E-02 6.1850E+00 1.2000E+02 7.37470E-02 9.09E-02 1.01134E-01 2.7080E-01 10 6.4358E-02 7.7230E+00 1.2000E+02 3.74930E-02 9.09E-02 3.55305E-02 -5.5234E-02 11 7.6658E-02 9.1990E+00 1.2000E+02 1.21660E-02 9.09E-02 1.11767E-02 -8.8516E-02 ----------------------------------------------------------------------------------------------------- RESIDUAL: Stats: min=-4.951E-01 max=6.391E-01, mean=1.0918E-01, stdev=3.3117E-01, var=1.0967E-01 Student's T test (mean=0, NDF=8): Pr>ABS(1.09344)= 3.0603E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.96933)=8.650E-01 (passed normality test) WEIGHTED RESIDUAL: Stats: min=-1.493E-01 max=1.927E-01, mean=3.2919E-02, stdev=9.9851E-02, var=9.9701E-03 Student's T test (mean=0, NDF=8): Pr>ABS(1.09344)= 3.0603E-01 (acceptable fit) Shapiro-Wilk's Test: (Pr<0.96933)=8.650E-01 (passed normality test) NOX=11, NPAR=2 CFE.SF: Cell survival data for A 549 cells. ========================================================================================= -------- EXPOSURE SUMMARY -------- ------------ ENDPOINT ------------- DATASET ID Duration Dose Dose Rate Fraction Fraction Standard CFE RADX (h) (Gy) (Gy/h) Killed Viable Error (%) ----------------------------------------------------------------------------------------- 1 1 3.8933E+00 2.3360E+00 6.0000E-01 3.10929E-01 6.89071E-01 1.00E+01 2 2 1.2255E+01 7.3530E+00 6.0000E-01 6.29277E-01 3.70723E-01 1.00E+01 3 3 2.0118E+01 1.2071E+01 6.0000E-01 8.00441E-01 1.99559E-01 1.00E+01 4 4 2.7985E+01 1.6791E+01 6.0000E-01 9.76672E-01 2.33280E-02 1.00E+01 5 5 3.5705E+01 2.1423E+01 6.0000E-01 9.90992E-01 9.00800E-03 1.00E+01 6 6 1.2233E-02 1.4680E+00 1.2000E+02 2.44984E-01 7.55016E-01 1.00E+01 7 7 2.4817E-02 2.9780E+00 1.2000E+02 6.26339E-01 3.73661E-01 1.00E+01 8 8 3.7650E-02 4.5180E+00 1.2000E+02 7.86501E-01 2.13499E-01 1.00E+01 9 9 5.1542E-02 6.1850E+00 1.2000E+02 9.26253E-01 7.37470E-02 1.00E+01 10 10 6.4358E-02 7.7230E+00 1.2000E+02 9.62507E-01 3.74930E-02 1.00E+01 11 11 7.6658E-02 9.1990E+00 1.2000E+02 9.87834E-01 1.21660E-02 1.00E+01 ----------------------------------------------------------------------------------------- CFE.DXA: Additional details from the cell survival assay (A 549). ===================================================================================== DATASET ID ---------- TIME (h) ----------- DOSE ---- LETHAL DAMAGE --- CFE RADX RADX TTP ETSI (Gy) #/(cell Gy) #/(cell) ------------------------------------------------------------------------------------- 1 1 3.893E+00 1.200E+01 4.000E+02 2.3360E+00 1.5942E-01 3.7241E-01 2 2 1.226E+01 1.200E+01 4.000E+02 7.3530E+00 1.3495E-01 9.9230E-01 3 3 2.012E+01 1.200E+01 4.000E+02 1.2071E+01 1.3351E-01 1.6116E+00 4 4 2.799E+01 1.200E+01 4.000E+02 1.6791E+01 2.2382E-01 3.7581E+00 5 5 3.570E+01 1.200E+01 4.000E+02 2.1423E+01 2.1984E-01 4.7096E+00 6 6 1.223E-02 1.200E+01 4.000E+02 1.4680E+00 1.9143E-01 2.8102E-01 7 7 2.482E-02 1.200E+01 4.000E+02 2.9780E+00 3.3056E-01 9.8441E-01 8 8 3.765E-02 1.200E+01 4.000E+02 4.5180E+00 3.4177E-01 1.5441E+00 9 9 5.154E-02 1.200E+01 4.000E+02 6.1850E+00 4.2152E-01 2.6071E+00 10 10 6.436E-02 1.200E+01 4.000E+02 7.7230E+00 4.2517E-01 3.2836E+00 11 11 7.666E-02 1.200E+01 4.000E+02 9.1990E+00 4.7930E-01 4.4091E+00 ------------------------------------------------------------------------------------- ETSI: Total elapsed time (after irradiation) before colonies are counted. TTP: Time available for repair before cells are plated. Total VC execution time (min): 0.13412 VC 2.00I 15-JUL-2003 01-AUG-2003 01:46:20 PM