VC 2.00H 10-JUL-2003 24-JUL-2003 02:50:50 PM by RD Stewart (trebor@purdue.edu) PROGRAM FILE INPUT/OUTPUT AND RUN-TIME OPTIONS: ================================================================================ CMD: vc lpl.inp ..\xdat\a549.xdat -c FNI: lpl.inp FNO: lpl.out RADX: ..\xdat\a549.radx (11 exposures) XDAT: ..\xdat\a549.xdat (11 experiments) -------------------------------------------------------------------------------- MCAT SUMMARY: BEST-FIT MODEL PARAMETERS (method = BCONF): ========================================================================================== --- PARAMETER --- ----- CONSTRAINTS ----- INITIAL OPTIMAL PID CID NAM Minimum Maximum GUESS VALUE 2001 CLRP BMR 0.00000E+00 1.00000E-02 1.00000E-04 1.0000000E-02 1101 IRAD FL 0.00000E+00 2.00000E+00 1.00000E-01 1.5610093E-01 2201 CLRP RHT 1.00000E-02 1.20000E+01 2.00000E+00 4.6943898E-02 ------------------------------------------------------------------------------------------ FIGURE OF MERIT (FOM): 2.98555E-02 (nfom=867, nsim=9537, nmx=11, nox=11, ndf=7, nap=3) COMBINED UNWEIGHTED RESIDUAL: Stats: min=-3.831E-01 max=7.202E-01, mean=7.8536E-02, stdev=3.3445E-01, var=1.1185E-01 Student's T test (NDF=7): Pr>ABS(0.77883)= 4.6159E-01 (accept fit) Shapiro-Wilk's Test: (Pr<0.87291)=8.297E-02 (accept normality) COMBINED WEIGHTED RESIDUAL: Stats: min=-1.155E-01 max=2.171E-01, mean=2.3680E-02, stdev=1.0084E-01, var=1.0169E-02 Student's T test (NDF=7): Pr>ABS(0.77883)= 4.6159E-01 (accept fit) Shapiro-Wilk's Test: (Pr<0.87291)=8.297E-02 (accept normality) QECK TABLE: Cell and tissue kinetic parameters. ================================================================================ Duration of cell cycle (TCC): 10.00000 h Potential doubling time (Tpot): 480.00000 h (= 20.000 days) Cell birth rate (AX): 1.6636E+00 cell/day (= LOG(2)/TCC) Cell death rate (BX): 1.6289E+00 cell/day [=AX-LOG(2)/TPOT] Net cell birth rate (AX-BX): 3.4657E-02 cell/day Initial Number of Cells: non-dividing: 1.00000E+00 (100.000% = 1-GF) dividing: 0.00000E+00 ( 0.000% = GF) total: 1.00000E+00 (100.000% total) Life Support Capacity of System: Initial system capacity utilization: 0.000000% (=F0) Peak cell density (cells/cm^3): 1.00000E+38 (=KAP) Tissue volume (cm^3): 1.00000E+00 (=VOL) Maximum number of cells (cells): 1.00000E+38 (=KAP*VOL) -------------------------------------------------------------------------------- LPLM TABLE: Summary of LPL parameters and related (derived) quantities. ======================================================================== G0/G1 DNA CONTENT: 5667.000 Mbp/cell = 3.482938E+12 Da/cell ENDOGENOUS DAMAGE: per (h-Da) per (h-Mbp) per (h-cell) DSB = 0.00000E+00 0.00000E+00 0.00000E+00 RADIATION DAMAGE: per (Gy-Da) per (Gy-Mbp) per (Gy-cell) DSB = 7.17785E-12 4.41151E-03 25.000000 FL = 4.48187E-14 2.75456E-05 0.156101 DAMAGE REPAIR/MISREPAIR: A0= 9.500000E-01 (=95.000%) (1-A0)= 5.000000E-02 (=5.000%) RHT= 0.046944 h (=2.82 minute) LAM= 1.4765437E+01 (1/h) BMR= 1.0000000E-02 (1/h) LAM/BMR= 1.4765437E+03 GAM= 0.250 ***** EQUIVALENT LQ MODEL PARAMETERS ***** alpha = 0.156101 (1/Gy) beta = 0.052911 (1/Gy^2) alpha/beta = 2.950270 (Gy) ------------------------------------------------------------------------ NOTE: 1 bp = 614.6 Da (amu) = 1.0205E-09 pg MCAT FIT: CELL SURVIVAL AS A FUNCTION OF DOSE AND DOSE RATE (A 549 cells). ===================================================================================================== -------- EXPOSURE SUMMARY -------- Duration Dose Dose Rate ---------------- SURVIVING FRACTION -------------- EXPERIMENT (h) (Gy) (Gy/h)* Measured Norm. Wt Predicted Residual ----------------------------------------------------------------------------------------------------- 1 3.8933E+00 2.3360E+00 6.0000E-01 6.89071E-01 9.09E-02 6.87627E-01 -2.1000E-03 2 1.2255E+01 7.3530E+00 6.0000E-01 3.70723E-01 9.09E-02 3.07521E-01 -2.0552E-01 3 2.0118E+01 1.2071E+01 6.0000E-01 1.99559E-01 9.09E-02 1.44286E-01 -3.8308E-01 4 2.7985E+01 1.6791E+01 6.0000E-01 2.33280E-02 9.09E-02 6.76762E-02 6.5530E-01 5 3.5705E+01 2.1423E+01 6.0000E-01 9.00800E-03 9.09E-02 3.21942E-02 7.2020E-01 6 1.2233E-02 1.4680E+00 1.2000E+02 7.55016E-01 9.09E-02 7.15398E-01 -5.5380E-02 7 2.4817E-02 2.9780E+00 1.2000E+02 3.73661E-01 9.09E-02 4.19389E-01 1.0904E-01 8 3.7650E-02 4.5180E+00 1.2000E+02 2.13499E-01 9.09E-02 2.08004E-01 -2.6417E-02 9 5.1542E-02 6.1850E+00 1.2000E+02 7.37470E-02 9.09E-02 8.46735E-02 1.2904E-01 10 6.4358E-02 7.7230E+00 1.2000E+02 3.74930E-02 9.09E-02 3.34076E-02 -1.2229E-01 11 7.6658E-02 9.1990E+00 1.2000E+02 1.21660E-02 9.09E-02 1.27407E-02 4.5106E-02 ----------------------------------------------------------------------------------------------------- RESIDUAL: Stats: min=-3.831E-01 max=7.202E-01, mean=7.8536E-02, stdev=3.3445E-01, var=1.1185E-01 Student's T test (NDF=7): Pr>ABS(0.77883)= 4.6159E-01 (accept fit) Shapiro-Wilk's Test: (Pr<0.87291)=8.297E-02 (accept normality) WEIGHTED RESIDUAL: Stats: min=-1.155E-01 max=2.171E-01, mean=2.3680E-02, stdev=1.0084E-01, var=1.0169E-02 Student's T test (NDF=7): Pr>ABS(0.77883)= 4.6159E-01 (accept fit) Shapiro-Wilk's Test: (Pr<0.87291)=8.297E-02 (accept normality) NOX=11, NPAR=3 CFE.SF: Cell survival data for A 549 cells. ========================================================================================= -------- EXPOSURE SUMMARY -------- ------------ ENDPOINT ------------- DATASET ID Duration Dose Dose Rate Fraction Fraction Standard CFE RADX (h) (Gy) (Gy/h) Killed Viable Error (%) ----------------------------------------------------------------------------------------- 1 1 3.8933E+00 2.3360E+00 6.0000E-01 3.10929E-01 6.89071E-01 1.00E+01 2 2 1.2255E+01 7.3530E+00 6.0000E-01 6.29277E-01 3.70723E-01 1.00E+01 3 3 2.0118E+01 1.2071E+01 6.0000E-01 8.00441E-01 1.99559E-01 1.00E+01 4 4 2.7985E+01 1.6791E+01 6.0000E-01 9.76672E-01 2.33280E-02 1.00E+01 5 5 3.5705E+01 2.1423E+01 6.0000E-01 9.90992E-01 9.00800E-03 1.00E+01 6 6 1.2233E-02 1.4680E+00 1.2000E+02 2.44984E-01 7.55016E-01 1.00E+01 7 7 2.4817E-02 2.9780E+00 1.2000E+02 6.26339E-01 3.73661E-01 1.00E+01 8 8 3.7650E-02 4.5180E+00 1.2000E+02 7.86501E-01 2.13499E-01 1.00E+01 9 9 5.1542E-02 6.1850E+00 1.2000E+02 9.26253E-01 7.37470E-02 1.00E+01 10 10 6.4358E-02 7.7230E+00 1.2000E+02 9.62507E-01 3.74930E-02 1.00E+01 11 11 7.6658E-02 9.1990E+00 1.2000E+02 9.87834E-01 1.21660E-02 1.00E+01 ----------------------------------------------------------------------------------------- CFE.DXA: Additional details from the cell survival assay (A 549). ===================================================================================== DATASET ID ---------- TIME (h) ----------- DOSE ---- LETHAL DAMAGE --- CFE RADX RADX TTP ETSI (Gy) #/(cell Gy) #/(cell) ------------------------------------------------------------------------------------- 1 1 3.893E+00 1.200E+01 4.000E+02 2.3360E+00 1.5942E-01 3.7241E-01 2 2 1.226E+01 1.200E+01 4.000E+02 7.3530E+00 1.3495E-01 9.9230E-01 3 3 2.012E+01 1.200E+01 4.000E+02 1.2071E+01 1.3351E-01 1.6116E+00 4 4 2.799E+01 1.200E+01 4.000E+02 1.6791E+01 2.2382E-01 3.7581E+00 5 5 3.570E+01 1.200E+01 4.000E+02 2.1423E+01 2.1984E-01 4.7096E+00 6 6 1.223E-02 1.200E+01 4.000E+02 1.4680E+00 1.9143E-01 2.8102E-01 7 7 2.482E-02 1.200E+01 4.000E+02 2.9780E+00 3.3056E-01 9.8441E-01 8 8 3.765E-02 1.200E+01 4.000E+02 4.5180E+00 3.4177E-01 1.5441E+00 9 9 5.154E-02 1.200E+01 4.000E+02 6.1850E+00 4.2152E-01 2.6071E+00 10 10 6.436E-02 1.200E+01 4.000E+02 7.7230E+00 4.2517E-01 3.2836E+00 11 11 7.666E-02 1.200E+01 4.000E+02 9.1990E+00 4.7930E-01 4.4091E+00 ------------------------------------------------------------------------------------- ETSI: Total elapsed time (after irradiation) before colonies are counted. TTP: Time available for repair before cells are plated. Total VC execution time (min): 0.43853 VC 2.00H 10-JUL-2003 24-JUL-2003 02:51:17 PM