VC 2.00H 10-JUL-2003 23-JUL-2003 07:10:00 PM by RD Stewart (trebor@purdue.edu) PROGRAM FILE INPUT/OUTPUT AND RUN-TIME OPTIONS: ================================================================================ CMD: vc lpl.inp ..\xdat\hx160c.xdat -c FNI: lpl.inp FNO: lpl.out RADX: ..\xdat\hx160c.radx (12 exposures) XDAT: ..\xdat\hx160c.xdat (12 experiments) -------------------------------------------------------------------------------- MCAT SUMMARY: BEST-FIT MODEL PARAMETERS (method = BCONF): ========================================================================================== --- PARAMETER --- ----- CONSTRAINTS ----- INITIAL OPTIMAL PID CID NAM Minimum Maximum GUESS VALUE 2001 CLRP BMR 0.00000E+00 1.00000E-02 1.00000E-04 8.6776838E-04 1101 IRAD FL 0.00000E+00 2.00000E+00 1.00000E-01 7.2507276E-02 2201 CLRP RHT 1.00000E-02 1.20000E+01 2.00000E+00 1.9717779E+00 ------------------------------------------------------------------------------------------ FIGURE OF MERIT (FOM): 1.62345E-02 (nfom=106, nsim=1272, nmx=12, nox=12, ndf=8, nap=3) COMBINED UNWEIGHTED RESIDUAL: Stats: min=-4.455E-01 max=2.608E-01, mean=5.1288E-02, stdev=1.9630E-01, var=3.8533E-02 Student's T test (NDF=8): Pr>ABS(0.90508)= 3.9186E-01 (accept fit) Shapiro-Wilk's Test: (Pr<0.85630)=4.118E-02 (reject normality) COMBINED WEIGHTED RESIDUAL: Stats: min=-1.286E-01 max=7.528E-02, mean=1.4806E-02, stdev=5.6667E-02, var=3.2111E-03 Student's T test (NDF=8): Pr>ABS(0.90508)= 3.9186E-01 (accept fit) Shapiro-Wilk's Test: (Pr<0.85630)=4.118E-02 (reject normality) QECK TABLE: Cell and tissue kinetic parameters. ================================================================================ Duration of cell cycle (TCC): 10.00000 h Potential doubling time (Tpot): 480.00000 h (= 20.000 days) Cell birth rate (AX): 1.6636E+00 cell/day (= LOG(2)/TCC) Cell death rate (BX): 1.6289E+00 cell/day [=AX-LOG(2)/TPOT] Net cell birth rate (AX-BX): 3.4657E-02 cell/day Initial Number of Cells: non-dividing: 1.00000E+00 (100.000% = 1-GF) dividing: 0.00000E+00 ( 0.000% = GF) total: 1.00000E+00 (100.000% total) Life Support Capacity of System: Initial system capacity utilization: 0.000000% (=F0) Peak cell density (cells/cm^3): 1.00000E+38 (=KAP) Tissue volume (cm^3): 1.00000E+00 (=VOL) Maximum number of cells (cells): 1.00000E+38 (=KAP*VOL) -------------------------------------------------------------------------------- LPLM TABLE: Summary of LPL parameters and related (derived) quantities. ======================================================================== G0/G1 DNA CONTENT: 5667.000 Mbp/cell = 3.482938E+12 Da/cell ENDOGENOUS DAMAGE: per (h-Da) per (h-Mbp) per (h-cell) DSB = 0.00000E+00 0.00000E+00 0.00000E+00 RADIATION DAMAGE: per (Gy-Da) per (Gy-Mbp) per (Gy-cell) DSB = 7.17785E-12 4.41151E-03 25.000000 FL = 2.08178E-14 1.27946E-05 0.072507 DAMAGE REPAIR/MISREPAIR: A0= 9.500000E-01 (=95.000%) (1-A0)= 5.000000E-02 (=5.000%) RHT= 1.971778 h (=118.31 minute) LAM= 3.5153411E-01 (1/h) BMR= 8.6776838E-04 (1/h) LAM/BMR= 4.0510131E+02 GAM= 0.250 ***** EQUIVALENT LQ MODEL PARAMETERS ***** alpha = 0.072507 (1/Gy) beta = 0.192853 (1/Gy^2) alpha/beta = 0.375972 (Gy) ------------------------------------------------------------------------ NOTE: 1 bp = 614.6 Da (amu) = 1.0205E-09 pg MCAT FIT: CELL SURVIVAL AS A FUNCTION OF DOSE AND DOSE RATE (HX 160C cells). ===================================================================================================== -------- EXPOSURE SUMMARY -------- Duration Dose Dose Rate ---------------- SURVIVING FRACTION -------------- EXPERIMENT (h) (Gy) (Gy/h)* Measured Norm. Wt Predicted Residual ----------------------------------------------------------------------------------------------------- 1 1.0729E+00 2.0600E+00 1.9200E+00 3.81739E-01 8.33E-02 4.40012E-01 1.3244E-01 2 2.0625E+00 3.9600E+00 1.9200E+00 1.31560E-01 8.33E-02 9.10153E-02 -4.4547E-01 3 2.5677E+00 4.9300E+00 1.9200E+00 3.56650E-02 8.33E-02 3.38458E-02 -5.3750E-02 4 3.0625E+00 5.8800E+00 1.9200E+00 1.12150E-02 8.33E-02 1.18213E-02 5.1286E-02 5 3.5469E+00 6.8100E+00 1.9200E+00 3.63700E-03 8.33E-02 3.97274E-03 8.4512E-02 6 4.0677E+00 7.8100E+00 1.9200E+00 1.08700E-03 8.33E-02 1.16879E-03 6.9978E-02 7 1.8667E-02 1.1200E+00 6.0000E+01 6.50261E-01 8.33E-02 7.31960E-01 1.1162E-01 8 3.4333E-02 2.0600E+00 6.0000E+01 3.12680E-01 8.33E-02 4.05965E-01 2.2979E-01 9 5.0000E-02 3.0000E+00 6.0000E+01 1.27644E-01 8.33E-02 1.72673E-01 2.6078E-01 10 6.6167E-02 3.9700E+00 6.0000E+01 5.23750E-02 8.33E-02 5.56612E-02 5.9039E-02 11 8.1500E-02 4.8900E+00 6.0000E+01 1.14530E-02 8.33E-02 1.53884E-02 2.5574E-01 12 9.7167E-02 5.8300E+00 6.0000E+01 3.88900E-03 8.33E-02 3.40994E-03 -1.4049E-01 ----------------------------------------------------------------------------------------------------- RESIDUAL: Stats: min=-4.455E-01 max=2.608E-01, mean=5.1288E-02, stdev=1.9630E-01, var=3.8533E-02 Student's T test (NDF=8): Pr>ABS(0.90508)= 3.9186E-01 (accept fit) Shapiro-Wilk's Test: (Pr<0.85630)=4.118E-02 (reject normality) WEIGHTED RESIDUAL: Stats: min=-1.286E-01 max=7.528E-02, mean=1.4806E-02, stdev=5.6667E-02, var=3.2111E-03 Student's T test (NDF=8): Pr>ABS(0.90508)= 3.9186E-01 (accept fit) Shapiro-Wilk's Test: (Pr<0.85630)=4.118E-02 (reject normality) NOX=12, NPAR=3 CFE.SF: Cell survival data for HX 160C cells. ========================================================================================= -------- EXPOSURE SUMMARY -------- ------------ ENDPOINT ------------- DATASET ID Duration Dose Dose Rate Fraction Fraction Standard CFE RADX (h) (Gy) (Gy/h) Killed Viable Error (%) ----------------------------------------------------------------------------------------- 1 1 1.0729E+00 2.0600E+00 1.9200E+00 6.18261E-01 3.81739E-01 1.00E+01 2 2 2.0625E+00 3.9600E+00 1.9200E+00 8.68440E-01 1.31560E-01 1.00E+01 3 3 2.5677E+00 4.9300E+00 1.9200E+00 9.64335E-01 3.56650E-02 1.00E+01 4 4 3.0625E+00 5.8800E+00 1.9200E+00 9.88785E-01 1.12150E-02 1.00E+01 5 5 3.5469E+00 6.8100E+00 1.9200E+00 9.96363E-01 3.63700E-03 1.00E+01 6 6 4.0677E+00 7.8100E+00 1.9200E+00 9.98913E-01 1.08700E-03 1.00E+01 7 7 1.8667E-02 1.1200E+00 6.0000E+01 3.49739E-01 6.50261E-01 1.00E+01 8 8 3.4333E-02 2.0600E+00 6.0000E+01 6.87320E-01 3.12680E-01 1.00E+01 9 9 5.0000E-02 3.0000E+00 6.0000E+01 8.72356E-01 1.27644E-01 1.00E+01 10 10 6.6167E-02 3.9700E+00 6.0000E+01 9.47625E-01 5.23750E-02 1.00E+01 11 11 8.1500E-02 4.8900E+00 6.0000E+01 9.88547E-01 1.14530E-02 1.00E+01 12 12 9.7167E-02 5.8300E+00 6.0000E+01 9.96111E-01 3.88900E-03 1.00E+01 ----------------------------------------------------------------------------------------- CFE.DXA: Additional details from the cell survival assay (HX 160C). ===================================================================================== DATASET ID ---------- TIME (h) ----------- DOSE ---- LETHAL DAMAGE --- CFE RADX RADX TTP ETSI (Gy) #/(cell Gy) #/(cell) ------------------------------------------------------------------------------------- 1 1 1.073E+00 1.200E+01 4.000E+02 2.0600E+00 4.6748E-01 9.6302E-01 2 2 2.062E+00 1.200E+01 4.000E+02 3.9600E+00 5.1220E-01 2.0283E+00 3 3 2.568E+00 1.200E+01 4.000E+02 4.9300E+00 6.7618E-01 3.3336E+00 4 4 3.062E+00 1.200E+01 4.000E+02 5.8800E+00 7.6369E-01 4.4905E+00 5 5 3.547E+00 1.200E+01 4.000E+02 6.8100E+00 8.2476E-01 5.6166E+00 6 6 4.068E+00 1.200E+01 4.000E+02 7.8100E+00 8.7379E-01 6.8243E+00 7 7 1.867E-02 1.200E+01 4.000E+02 1.1200E+00 3.8427E-01 4.3038E-01 8 8 3.433E-02 1.200E+01 4.000E+02 2.0600E+00 5.6436E-01 1.1626E+00 9 9 5.000E-02 1.200E+01 4.000E+02 3.0000E+00 6.8617E-01 2.0585E+00 10 10 6.617E-02 1.200E+01 4.000E+02 3.9700E+00 7.4290E-01 2.9493E+00 11 11 8.150E-02 1.200E+01 4.000E+02 4.8900E+00 9.1401E-01 4.4695E+00 12 12 9.717E-02 1.200E+01 4.000E+02 5.8300E+00 9.5190E-01 5.5496E+00 ------------------------------------------------------------------------------------- ETSI: Total elapsed time (after irradiation) before colonies are counted. TTP: Time available for repair before cells are plated. Total VC execution time (min): 6.66667E-02 VC 2.00H 10-JUL-2003 23-JUL-2003 07:10:04 PM