VC 2.00H 10-JUL-2003 23-JUL-2003 04:24:51 PM by RD Stewart (trebor@purdue.edu) PROGRAM FILE INPUT/OUTPUT AND RUN-TIME OPTIONS: ================================================================================ CMD: vc rmr.inp ..\xdat\hx156c.xdat -c FNI: rmr.inp FNO: rmr.out RADX: ..\xdat\hx156c.radx (11 exposures) XDAT: ..\xdat\hx156c.xdat (11 experiments) -------------------------------------------------------------------------------- MCAT SUMMARY: BEST-FIT MODEL PARAMETERS (method = BCONF): ========================================================================================== --- PARAMETER --- ----- CONSTRAINTS ----- INITIAL OPTIMAL PID CID NAM Minimum Maximum GUESS VALUE 2001 CLRP BMR 0.00000E+00 1.00000E-02 1.00000E-04 3.1385898E-04 2401 CLRP PHI 0.00000E+00 1.00000E+00 1.00000E-02 7.6643567E-03 2201 CLRP RHT 1.00000E-02 1.20000E+01 2.00000E+00 1.1870581E+00 ------------------------------------------------------------------------------------------ FIGURE OF MERIT (FOM): 6.73623E-03 (nfom=96, nsim=1056, nmx=11, nox=11, ndf=7, nap=3) COMBINED UNWEIGHTED RESIDUAL: Stats: min=-1.191E-01 max=1.272E-01, mean=-2.9253E-03, stdev=7.7655E-02, var=6.0302E-03 Student's T test (NDF=7): Pr>ABS(0.12494)= 9.0408E-01 (accept fit) Shapiro-Wilk's Test: (Pr<0.87516)=8.844E-02 (accept normality) COMBINED WEIGHTED RESIDUAL: Stats: min=-3.591E-02 max=3.835E-02, mean=-8.8202E-04, stdev=2.3414E-02, var=5.4820E-04 Student's T test (NDF=7): Pr>ABS(0.12494)= 9.0408E-01 (accept fit) Shapiro-Wilk's Test: (Pr<0.87516)=8.844E-02 (accept normality) QECK TABLE: Cell and tissue kinetic parameters. ================================================================================ Duration of cell cycle (TCC): 10.00000 h Potential doubling time (Tpot): 480.00000 h (= 20.000 days) Cell birth rate (AX): 1.6636E+00 cell/day (= LOG(2)/TCC) Cell death rate (BX): 1.6289E+00 cell/day [=AX-LOG(2)/TPOT] Net cell birth rate (AX-BX): 3.4657E-02 cell/day Initial Number of Cells: non-dividing: 1.00000E+00 (100.000% = 1-GF) dividing: 0.00000E+00 ( 0.000% = GF) total: 1.00000E+00 (100.000% total) Life Support Capacity of System: Initial system capacity utilization: 0.000000% (=F0) Peak cell density (cells/cm^3): 1.00000E+38 (=KAP) Tissue volume (cm^3): 1.00000E+00 (=VOL) Maximum number of cells (cells): 1.00000E+38 (=KAP*VOL) -------------------------------------------------------------------------------- RMRM TABLE: Summary of RMR parameters and related (derived) quantities. ======================================================================== G0/G1 DNA CONTENT: 5667.000 Mbp/cell = 3.482938E+12 Da/cell ENDOGENOUS DAMAGE: per (h-Da) per (h-Mbp) per (h-cell) DSB = 0.00000E+00 0.00000E+00 0.00000E+00 DAMAGE FORMATION: per (Gy-Da) per (Gy-Mbp) per (Gy-cell) DSB = 7.17785E-12 4.41151E-03 25.000000 DAMAGE REPAIR/MISREPAIR: A0= 0.000000E+00 (=0.000%) (1-A0)*PHI= 7.664357E-03 (=0.766%) (1-A0)*(1-PHI)= 9.923356E-01 (=99.234%) RHT= 1.187058 h (=71.22 minute) LAM= 5.8392020E-01 (1/h) BMR= 3.1385898E-04 (1/h) LAM/BMR= 1.8604540E+03 GAM= 0.250 ***** EQUIVALENT LQ MODEL PARAMETERS ***** alpha = 0.191609 (1/Gy) beta = 0.040705 (1/Gy^2) alpha/beta = 4.707251 (Gy) ------------------------------------------------------------------------ NOTE: 1 bp = 614.6 Da (amu) = 1.0205E-09 pg MCAT FIT: CELL SURVIVAL AS A FUNCTION OF DOSE AND DOSE RATE (HX 156C cells). ===================================================================================================== -------- EXPOSURE SUMMARY -------- Duration Dose Dose Rate ---------------- SURVIVING FRACTION -------------- EXPERIMENT (h) (Gy) (Gy/h)* Measured Norm. Wt Predicted Residual ----------------------------------------------------------------------------------------------------- 1 1.0885E+00 2.0900E+00 1.9200E+00 6.49878E-01 9.09E-02 5.80717E-01 -1.1910E-01 2 2.1146E+00 4.0600E+00 1.9200E+00 3.14125E-01 9.09E-02 2.93059E-01 -7.1882E-02 3 3.1302E+00 6.0100E+00 1.9200E+00 1.41363E-01 9.09E-02 1.36871E-01 -3.2817E-02 4 4.1510E+00 7.9700E+00 1.9200E+00 5.32060E-02 9.09E-02 6.09171E-02 1.2658E-01 5 5.1875E+00 9.9600E+00 1.9200E+00 2.65170E-02 9.09E-02 2.61499E-02 -1.4040E-02 6 6.2031E+00 1.1910E+01 1.9200E+00 1.15140E-02 9.09E-02 1.12801E-02 -2.0736E-02 7 3.5000E-02 2.1000E+00 6.0000E+01 5.80825E-01 9.09E-02 5.61357E-01 -3.4680E-02 8 6.7500E-02 4.0500E+00 6.0000E+01 2.27726E-01 9.09E-02 2.43615E-01 6.5222E-02 9 1.0000E-01 6.0000E+00 6.0000E+01 7.05970E-02 9.09E-02 8.08850E-02 1.2719E-01 10 1.3250E-01 7.9500E+00 6.0000E+01 2.15530E-02 9.09E-02 2.09120E-02 -3.0654E-02 11 1.6617E-01 9.9700E+00 6.0000E+01 4.13500E-03 9.09E-02 4.02522E-03 -2.7273E-02 ----------------------------------------------------------------------------------------------------- RESIDUAL: Stats: min=-1.191E-01 max=1.272E-01, mean=-2.9253E-03, stdev=7.7655E-02, var=6.0302E-03 Student's T test (NDF=7): Pr>ABS(0.12494)= 9.0408E-01 (accept fit) Shapiro-Wilk's Test: (Pr<0.87516)=8.844E-02 (accept normality) WEIGHTED RESIDUAL: Stats: min=-3.591E-02 max=3.835E-02, mean=-8.8202E-04, stdev=2.3414E-02, var=5.4820E-04 Student's T test (NDF=7): Pr>ABS(0.12494)= 9.0408E-01 (accept fit) Shapiro-Wilk's Test: (Pr<0.87516)=8.844E-02 (accept normality) NOX=11, NPAR=3 CFE.SF: Cell survival data for HX 156C cells. ========================================================================================= -------- EXPOSURE SUMMARY -------- ------------ ENDPOINT ------------- DATASET ID Duration Dose Dose Rate Fraction Fraction Standard CFE RADX (h) (Gy) (Gy/h) Killed Viable Error (%) ----------------------------------------------------------------------------------------- 1 1 1.0885E+00 2.0900E+00 1.9200E+00 3.50122E-01 6.49878E-01 1.00E+01 2 2 2.1146E+00 4.0600E+00 1.9200E+00 6.85875E-01 3.14125E-01 1.00E+01 3 3 3.1302E+00 6.0100E+00 1.9200E+00 8.58637E-01 1.41363E-01 1.00E+01 4 4 4.1510E+00 7.9700E+00 1.9200E+00 9.46794E-01 5.32060E-02 1.00E+01 5 5 5.1875E+00 9.9600E+00 1.9200E+00 9.73483E-01 2.65170E-02 1.00E+01 6 6 6.2031E+00 1.1910E+01 1.9200E+00 9.88486E-01 1.15140E-02 1.00E+01 7 7 3.5000E-02 2.1000E+00 6.0000E+01 4.19175E-01 5.80825E-01 1.00E+01 8 8 6.7500E-02 4.0500E+00 6.0000E+01 7.72274E-01 2.27726E-01 1.00E+01 9 9 1.0000E-01 6.0000E+00 6.0000E+01 9.29403E-01 7.05970E-02 1.00E+01 10 10 1.3250E-01 7.9500E+00 6.0000E+01 9.78447E-01 2.15530E-02 1.00E+01 11 11 1.6617E-01 9.9700E+00 6.0000E+01 9.95865E-01 4.13500E-03 1.00E+01 ----------------------------------------------------------------------------------------- CFE.DXA: Additional details from the cell survival assay (HX 156C). ===================================================================================== DATASET ID ---------- TIME (h) ----------- DOSE ---- LETHAL DAMAGE --- CFE RADX RADX TTP ETSI (Gy) #/(cell Gy) #/(cell) ------------------------------------------------------------------------------------- 1 1 1.089E+00 1.200E+01 4.000E+02 2.0900E+00 2.0621E-01 4.3097E-01 2 2 2.115E+00 1.200E+01 4.000E+02 4.0600E+00 2.8521E-01 1.1580E+00 3 3 3.130E+00 1.200E+01 4.000E+02 6.0100E+00 3.2553E-01 1.9564E+00 4 4 4.151E+00 1.200E+01 4.000E+02 7.9700E+00 3.6808E-01 2.9336E+00 5 5 5.188E+00 1.200E+01 4.000E+02 9.9600E+00 3.6445E-01 3.6300E+00 6 6 6.203E+00 1.200E+01 4.000E+02 1.1910E+01 3.7483E-01 4.4642E+00 7 7 3.500E-02 1.200E+01 4.000E+02 2.1000E+00 2.5872E-01 5.4331E-01 8 8 6.750E-02 1.200E+01 4.000E+02 4.0500E+00 3.6534E-01 1.4796E+00 9 9 1.000E-01 1.200E+01 4.000E+02 6.0000E+00 4.4179E-01 2.6508E+00 10 10 1.325E-01 1.200E+01 4.000E+02 7.9500E+00 4.8267E-01 3.8372E+00 11 11 1.662E-01 1.200E+01 4.000E+02 9.9700E+00 5.5048E-01 5.4883E+00 ------------------------------------------------------------------------------------- ETSI: Total elapsed time (after irradiation) before colonies are counted. TTP: Time available for repair before cells are plated. Total VC execution time (min): 5.26167E-02 VC 2.00H 10-JUL-2003 23-JUL-2003 04:24:54 PM