In a previous post I generate pie charts of the 2017 Oly gonad stage and sex

Here, I run some quick stats on the gonad stage and sex data to confirm that my visually determined differences in maturation between 6degC low pH vs. 6degC ambient pH is, indeed, statistically different. I performed these analyses in R in my Histology-Pie-Charts.R script.

I created contingency tables to analyze differences in Sex and Stage, then ran chi-squared tests on each.

Contingency Table – All populations, by Sex

Chi-squared results: X-squared = 9.6132, df = 8, p-value = 0.2932 not significantly different

A warning was printed along with these results… “Warning message: In chisq.test(CT.Sex) : Chi-squared approximation may be incorrect”. So, I ran a Fisher’s Exact Test for Count Data as well: p-value = 0.2942
Oly-Gonad-Contingency-Table-Sex

Contingency Table – All populations, by Stage

Chi-squared results: X-squared = 16.444, df = 4, p-value = 0.002478 significantly different
Oly-Gonad-Contingency-Table-Stage

Stages are significantly different between treatments; take a look at this correlation plot, where blue and red bubbles signify more and fewer gonads at each stage than expected, respetively. This indicates that low pH treatment resulted in more early-stage gonad.

Oly-stage-correlation-plot

Let’s dial down into each poplulation. Bear with me here, there are a ton of contingency tables and stats results:

Tables, by Sex:

SN
K
HL
NF

Tables, by STAGE:

SN
K
HL
NF

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Oly gonad statistics
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