Vector: Bluescript KS (3 Kb)
Insert: pXE, from homologue of F-spondin (aa's 300-807)
Insert size: 1.5 Kb
Cloning site: EcoR1
Antisense probe cut with Hind III and transcribe with T7.
Notes for Use:
Contains sequences from amino acids 300-807.
1.5 Kb cDNA in EcoR1 site with Bluescript KS.
For antisense in situ probe cut with Hind III and use T7 RNA polymerase. For sense probe, use T3.