Vector: pGem4
Insert: RACE'd wnt-8
Insert size: ~600 bp
Cloning sites: EcoR1, Xba1
Sense probe cut with Xba 1, transcribe with SP6.
Antisense probe cut with EcoR1, transcribe with T7.
Plasmid History
Constructed by Greg Kelly on date of 10/93, see page 68 in Book 2
Species of Sequence Origin: Zebrafish
Notes for Use:
Long 5' end of wnt-8 good in situ probe