Expression Construct Name: Wnt-4

RETURN TO ZEBRAFISH PROBES LIST

RETURN TO ZEBRAFISH EXPRESSION LIST Vector: T7Ts
Insert: wnt-4 (zebrafish) cDNA
Insert size: 1.3 Kb
Cloning sites: EcoRV and Spe1
To make an in situ probe, linearize with EcoRV and transcribe with SP6.
To make sense RNA, linearize with Xba1 and transcribe with T7.

Plasmid History
Constructed by Anne Ungar on date of 6/8/93
Species of Sequence Origin: Zebrafish

Notes for Use:
Note presence of R1, Sma, BamH1 sites brought in from pBSSK during construction. The 1274 nucleotide Nsi1 fragment containing the complete wnt-4 coding region was ligated into the Pst1 site of pBSSK (zf4 in pBSSK clone 5). The 5' end is near the Kpn1 site. A 1.3 Kb fragment was cut out with EcoRV and Spe1, isolated and ligated into EcoRV/Spe1 cut pT7Ts.

Citation: Ungar et al., Mechanisms of Development 52 (1995)