Plasmid: pGEM1
Description: cDNA clone of wnt-1 for DIG labelled probes cloned by G.
Kelly.
Antisense linearize with EcoR1 and transcribe with SP6 RNA
polyermase.
Sense linearize with HindIII and transcribe with T7 RNA polymerase.
Hydrolyze probes for 12 minutes according to Harland, 1991