Antisense are generated as listed:
Xenopus banded hedgehog:
Linearize clone XIL in Bluescript KS with BamH1 and transcribe with T3. This is a ~2.5 Kb EcoR1 insert.
Xenopus sonic hedgehog:
Linearize clone X32 in Bluescript KS with Bam H1 and transcribe with T3. This is a ~2.5 Kb EcoR1 insert.
Xenopus cephalic hedgehog:
Linearize clone X30 in pT7TS with Sal 1 and transcribe with T7.