RT-PCR Primers for Anterposterior Neural Ectoderm



























MARKER



FORWARD AND REVERSE PRIMERS


EXPRESSION



REFERENCE






N-CAM



F  5'CACAGTTCCACCAAATGC3'

R  5'GGAATCAAGCGGTACAGA3'


pan-neural



Hemmati-Brivanlou and Melton, 1994






NRP-1



F  5'ACAACTGGAAGCACTGGA3'

R  5'TCTTATTCCAGAGGAACC3'


pan-neural



Hemmati-Brivanlou and Melton, 1994






Xag-1



F  5'CTGACTGTCCGATCAGAC3'

R  5'GAGTTGCTTCTCTGGCAT3'


cement gland



Lai et al., 1995






Xanf-2



F  5'ACTGACCTACAAGAGAGAAC3'

R  5'AGTGCATCATTGTTCCACAG3'


anterior pituitary



Lai et al., 1995






Otx-A



F  5'CATCGGACATAAAGCAGCTCATC3'

R 5'CTTTCCCTCCTCTGTTTCCTGG3'


forebrain & retina expression (tailbud)



Lai et al., 1995






En-2



F  5'CGGAATTCATCAGGTCCGAGATC3'

R  5'GCGGATCCTTTGAAGTGGTCGCG3'


midbrain/hindbrain boarder



Hemmati-Brivanlou and Melton, 1994






Krox-20



F  5'AACCGCCCCAGTAAGACC3'

R  5'GTGTCAGCCTGTCCTGTTAG3'


rhombomeres 3 & 5 of the hindbrain



Hemmati-Brivanlou and Melton, 1994






Hox-B9
(XlHbox-6)



F  5'TACTTACGGGCTTGGCTGGA3'

R  5'AGCGTGTAACCAGTTGGCTG3'


spinal cord



Hemmati-Brivanlou and Melton, 1994











Control RT-PCR Primers














MARKER



FORWARD AND REVERSE PRIMERS


EXPRESSION



REFERENCE






EF1-alpha



F  5'CAGATTGGTGCTGGATATGC3'

R  5'ACTGCCTTGATGACTCCTAG3'


loading control



Hemmati-Brivanlou and Melton, 1994






Muscle actin



F  5'GCTGACAGAATGCAGAAG3'

R  5'TTGCTTGGAGGAGTGTGT3'


dorsal mesoderm



Hemmati-Brivanlou and Melton, 1994









PLEASE NOTE:  Other sequences are available (such as dorsal/ventral markers for mesoderm and Wnt-specific primers) in the following reference -




The primer sets listed above are those that are currently in use in our lab (i.e. they have been tested and work well!)