Construct Name: pCSKA

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Vector: pCSKA
Unique cloning sites: Cla1, HindIII, EcoR1, Sma1, BamH1
In vitro transcription: none


Plasmid History
pCSKA-CAT from Richard Harland, promoter from Hugh Woodland, Jan Christian removed CAT sequence.

Notes for Use:
Used for transient expression in vivo after midblastula transition in Xenopus, though spatially mosiac. See Christian and Moon, 1993, Genes and Development 7:13-28 for RNA blots of expression from plasmid as well as c-myc immunolocalization of proteins expressed from plasmid.