Vector: SP64T (3Kb)
Insert: coding region of Xenopus goosecoid in SP64T in sense (#9) & antisense (#2) orientation
Insert size: 850 bp
Cloning sites: Bgl II, Bam H1
Transcription: linearize with EcoR1 and transcribe with SP6.
Notes for Use
XL-1 host strain and Amp resistant SP64T
goosecoid cloned and characterized by lab of E. DeRobertis, UCLA