Probe Construct Name:
Zebrafish Axial (6T1N)

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Vector: pBluescript II KS
Insert: Full length axial cDNA cloned into the Not1 site of pBluescript
Insert size: 1.75 Kb
Cloning site: Not1
Antisense probe cut with Dra1 and transcribe with T3 RNA polymerase.

Plasmid History
Constructed by P. Blader, plasmid made by Phil Ingham on date of 11/94
Species of Sequence Origin: Zebrafish

Notes for Use:
Amp resistant pBluescript, host strain XL-1 blue
The enzymes listed are single cutters.