zebrafish cystathionine beta-synthase cDNA (likely full-lenght, at least contains full coding sequence) in pSPORT1. clone 6h9 from the microarray screen 1st round (Matthew clark normalized late somitogenesis / 24 hpf / liver library).
expressed pretty ubiquitously early, but stronger in axail mesoderm during gastrulation. later most prominently in eyes and somites.
for antisense: Asp718, SP6
LOCUS Gil304.pSP 6966 bp DNA circular 23-APR-2003
DEFINITION Complementary copy of 6-H9 pSPORT1 full.
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT VNTDATE|285711355|
COMMENT VNTDBDATE|300655615|
COMMENT VNTNAME|Gil304.pSPORT1 cbs|
COMMENT VNTAUTHORNAME|Demo User|
FEATURES Location/Qualifiers
promoter complement(3346..3362)
/vntifkey="30"
/label=SP6
promoter 352..368
/vntifkey="30"
/label=T7
CDS 4115..4975
/vntifkey="4"
/label=AmpR
misc_feature 418..3287
/vntifkey="21"
/label=EST\6-H9\insert
CDS 488..2281
/vntifkey="4"
/label=cystationine\beta-synthase\ORF
rep_origin complement(3525..3985)
/vntifkey="33"
/label=f1\ori
BASE COUNT 1720 a 1674 c 1766 g 1806 t
ORIGIN
1 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg
61 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca
121 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
181 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
241 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
301 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctaatacgac
361 tcactatagg gaaagctggt acgcctgcag gtaccggtcc ggaattcccg ggtcgaccca
421 cgcgtccgtc tctgtaatcg gacctccggc tctcaagttt ctctccatct gatagtcagg
481 gtaaaccatg ccagcagtac catctagttc agaatcagtt ggcactggcc ccgtctgccc
541 acatgctgcc aaaatgctct caaatgctgc aaatggagag tctggcatca acggggatgc
601 tgacgatgtc ttcaacggaa aggctggaca caagctgaat gaaaccaacg ggcgccactt
661 aaagagtctg aacggacagc acatggagaa gggctccgag gtcagcatgg agaggaaatg
721 gattcgaccg gatttgccca gcagatgcac ctggaagctt ggtgacctga atatggagtc
781 accccacaac cactaccaac ggacaaagac gtccggtatc ctccccaaca tcttaagcaa
841 gattggagag accccaatgg ttcgcatgaa caagattccc aaagcttttg gcctcaagtg
901 tgaactcttg gctaaatgcg agttcttcaa tgctggtggc agtgtgaagg acagaatcag
961 cttgcgaatg atagaagatg ctgaacgtga tggcaccctc aaacctggag acaccatcat
1021 tgagcctaca tcaggaaaca caggtatcgg tcttgctctg gcagcagcgg tcaaaggtta
1081 tcgctgcatt attgtcatgc ctgagaagat gagcatggaa aaggtggatg tgctccgtgc
1141 tctgggtgct gagattgttc gtaccccaac ctcagcccgc ttcgactctc ctgagtctca
1201 tgtgggggtc gcgtggcgtt tgaagaatga gattccaaat gctcacattc ttgaccagta
1261 ccgcaatccc agtaaccccc ttgcccacta tgacaccact gcagaggaga tcctggagca
1321 gtgtggcggt aaaatagaca tgctggtcgc aggagctggc actggtggca ccatcactgg
1381 catcgctcgc aagctaaagg agaaatgccc tgatatcaag atcattggag tggatccgga
1441 gggctctatt ctagctgagc cagacgagtt gaataagaca gataagactc agtatgaggt
1501 tgaaggcatc gggtatgatt tcatccccac tgttctggac agatctgtgg tggataattg
1561 gtacaagtcc aatgatgagg agtccttcgc catgtctcgt atgctcatca gagaggaggg
1621 tcttctgtgc ggtggtagtt ctggcacagc catgtctgca gcagtcaacg tagccaaaga
1681 gcttaaggaa ggccagcgct gtgttgtcat tctacccgac tccatccgca actacatgtc
1741 taaattcctc agtgacaagt ggatgtttga aaagggcttc atgagcgagg aggatcttgt
1801 tgtcaataag ccatggtggt ggaatttgac tcttcaggag cttcgcctct cggctccact
1861 gacggtgctt cctttggtgt ccatcaagaa gaccattcag atcctcaaag aaaaggcttt
1921 tgaccaggct cctgtggtgg acgaggcggg gcatattctg gggatggtca cgttggggaa
1981 catgctcgcg tctgttctgg ctggcaaggt cagaccatcc gatcccataa gcaaagtgct
2041 ctacaaacag tttaaacagg tgcgtctgac tgataacctt ggcaaactct cacgtatcct
2101 tgagacggat catttcgccc ttgttgtgca cgaacagatt cagtacatga gtgacggatc
2161 ccccaagatg aggcagatgg tgttcggggt ggtgacggcc attgacctgc tcaactacgt
2221 gaccatgcgt gagcgaaggg aacgcacgct gtctgagtgc tcgttgtcag aagatcagtg
2281 aacaacctga tcacaattct ggagcaaact atttttccat cttaattttg tatttttctt
2341 ttgtagctta tttctctgca agtggcacgc tccatttcaa gaactatgct ttttatatat
2401 gagcctgtca gtagctttac atgtcatttg actgatctgt agtctgtgtt cactggatcc
2461 atttttaaaa aatcttcagt ctctaatatt tagcttttgt tcagtttagg tgctttatta
2521 gtcatgatga agtaatctta caggtcaatc agctcccatt tgcagatgtg tttacgttgc
2581 agatttgatt ttgattaagg tgtatatatt tacatgaatc tattttattc tttgaaaaac
2641 tcagtaggct atgcggtttg gtaggatttg gtttggttta ggtgaagtgc tttattcagg
2701 tgatctcttg aatgcagcgc cgtctagtgg cagtggcagt ttacagcagg cgtggacact
2761 ttactttctt caatcatgct cttctaagca caacgatccc tccgctacac gtggagagct
2821 ttacaggcat gactggtcat gattgacaaa gcaaacaatt cgtgtgtttg ggaaaccaaa
2881 ctacacccag atggggtgac ttggtatttt tcttgtatta aaacaagaca tgattttgag
2941 tgtaaataag tgacggtggt ttgaaattac tttaaacgca ccacacttgt gtttaagaaa
3001 tttgatcacg cttttcattt ttctatgttc ttttggtctt gtaaatcaaa actgtaattt
3061 atggaggctg aataagattg tgtgtatata agagttcatg tgagtgaatg aatgtttggg
3121 gttgctctgc atgctgctgg aggtctctgc ctcaacagaa cgtcttattt ttctgttatg
3181 aatctcatgt cgtttgattt gcttttcatt gaatcggatt gctgtaataa tatgtactag
3241 ttacaaataa aggctttatt tacttaaaaa aaaaaaaaaa aaaaagggcg gccgctctag
3301 aggatccaag cttacgtacg cgtgcatgcg acgtcatagc tcttctatag tgtcacctaa
3361 attcaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
3421 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
3481 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
3541 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
3601 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
3661 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
3721 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
3781 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
3841 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
3901 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
3961 caaaatatta acgtttacaa tttcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
4021 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
4081 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
4141 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
4201 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
4261 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
4321 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
4381 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
4441 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
4501 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
4561 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
4621 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
4681 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
4741 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
4801 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
4861 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
4921 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
4981 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
5041 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
5101 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
5161 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
5221 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
5281 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
5341 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
5401 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
5461 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
5521 atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag
5581 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
5641 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
5701 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
5761 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
5821 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
5881 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
5941 ccccgcgcgt tggccgattc attaatgcag agcttgcaat tcgcgcgcga aggcgaagcg
6001 gcatttacgt tgacaccatc gaatggcgca aaacctttcg cggtatggca tgatagcgcc
6061 cggaagagag tcaattcagg gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag
6121 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
6181 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
6241 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
6301 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
6361 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
6421 tgcacaatct tctcgcgcaa cgggtcagtg ggctgatcat taactatccg ctggatgacc
6481 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
6541 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
6601 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
6661 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
6721 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
6781 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
6841 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
6901 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
6961 aggatt
//