zebrafish Isthmin cDNA, clone 27j15 from the microarray screen 1st round (Matthew clark normalized late somitogenesis / 24 hpf / liver library) in pSPORT1. A clone that is slightly longer at the 5' end was identified in the 2nd array round, so this is not exactly full-length. for antisense probe synthesis.
expressed on dorsal side early, in a dorsal high to ventral low gradient in margin, during gastrulation around axial mesoderm, later strongly at MHB.
for antisense: Asp718, SP6
Plasmid History
Species of Sequence Origin: Zebrafish
Citation: G WEIDINGER and R. T. Moon (2005). Development
LOCUS Gil303.pSP 6912 bp DNA circular 12-JAN-2005
DEFINITION Complementary copy of 27-j15 pSPORT1 full.
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT VNTDATE|286401107|
COMMENT VNTDBDATE|363285501|
COMMENT VNTNAME|Gil303.pSPORT1 27j15|
COMMENT VNTAUTHORNAME|Gilbert Weidinger|
COMMENT VNTAUTHOREML|gweidin@u.washington.edu|
COMMENT VNTOAUTHORNAME|Demo User|
FEATURES Location/Qualifiers
promoter complement(3301..3317)
/vntifkey="30"
/label=SP6
promoter 361..377
/vntifkey="30"
/label=T7
CDS 4070..4930
/vntifkey="4"
/label=AmpR
misc_feature 465..3242
/vntifkey="21"
/label=27-j15\insert
rep_origin complement(3480..3940)
/vntifkey="33"
/label=f1\ori
CDS 1175..2560
/vntifkey="4"
/label=zfish\Isthmin\ORF
misc_feature 478..480
/vntifkey="21"
/label=1st\ATG
misc_feature 493..495
/vntifkey="21"
/label=2nd\ATG\(good\Kozak,\but\short\ORF)
misc_feature 538..540
/vntifkey="21"
/label=3rd\ATG
misc_feature 575..577
/vntifkey="21"
/label=4th\ATG
misc_feature 685..687
/vntifkey="21"
/label=5th\ATG
misc_feature 689..691
/vntifkey="21"
/label=6th\ATG
misc_feature 700..702
/vntifkey="21"
/label=7th\ATG
misc_feature 703..705
/vntifkey="21"
/label=8th\ATG
misc_feature 707..709
/vntifkey="21"
/label=9th\ATG
misc_feature 792..794
/vntifkey="21"
/label=10th\ATG
misc_feature 817..819
/vntifkey="21"
/label=11th\ATG
misc_feature 822..824
/vntifkey="21"
/label=12th\ATG\(good\Kozak,\but\short\ORF)
misc_feature 1175..1177
/vntifkey="21"
/label=13th\ATG\=\good\Kozak,\start\of\Isthmin
exon 1311..1528
/vntifkey="61"
/label=Exon\n
exon 1529..1808
/vntifkey="61"
/label=Exon\n+1
exon 1809..1952
/vntifkey="61"
/label=Exon\n+2
exon 1953..2042
/vntifkey="61"
/label=Exon\n+3
exon 2043..2822
/vntifkey="61"
/label=Exon\n+4
BASE COUNT 1799 a 1698 c 1766 g 1649 t
ORIGIN
1 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg
61 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc
121 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
361 taatacgact cactataggg aaagccacag cccgctattg catccgctcc atgctcacgc
421 tggtggtacg cctgcaggta ccggtccgga attcccgggt cgacggaact ttggatcatg
481 atcacgttga agatgggctt tacgcttcat ccgtgaagac ttgggcgttt ggagaggatg
541 acagcacttt tgcgcaacac acagaagcgc agcaatgtat ccatctcaga agttttatat
601 tagagaggtg aacaacccac gaagatctga gcttgcttga cctggagtca ttagtgtttt
661 atttcatttt ggaaaaaaat aagaatgaat gatctcagaa tgatgcatga attacctgcg
721 cacaagtaaa actgacccca aataaagtag taaaacgtcg catctcaacc aataaagtgc
781 attttattct gatgtaaaaa gaaagtgaat acctcaatgg aatggtacac gactttggag
841 tttttgggac ttacttttct attttgtaac tgtaaatcga gttctttttg gacatcagga
901 ttaccccact tttaagttca ggagttatag atcacccatt gatctgattg gttttcaaac
961 gtggagtgat tgggcttctt cgagggcatt tccagccact ctttttcccg tgagtattgc
1021 tcttctggac ccagaggtgg ctccgcagtg gtgttgcggc tcctcgtccg cggcggatcc
1081 cgagctcgga gtgtctgcag cggcaggagg cagtagcgga agcccggcag cggcggcggc
1141 ggcgcagggt cctaagagcg ctcgggtgaa gaggatggtg cgtctggcgg cggagctgct
1201 gctgctgttg ggactccttc ttctgacctt acacatcacg gtgttgcgga gcagcccgct
1261 gcagcacggg aatgacacgg tgagcttgga gcaagactcg cgggtggccg agaataatgt
1321 gaatgcagat agcagctcct cagttcagct ggggccaggt gacagacaga ctcgggtggc
1381 tcatattcca gcatctcagc cttgggctca gagtcccggg acagggggca gcctgcagcg
1441 agacggccct ggagctttcc tgctggacct gcagaacttt cctgatctct ccaaagcaga
1501 tataaatggg caaaacccca atatacaggt caccatagaa gtggtggaca gtctggaagg
1561 ttctgagcca gagaagggaa tgcgtaaaga aaacaagcct ggctgggcag ctcctaactg
1621 gaggaactgg tggcagcgtt catcatcctc gtcctcatct tctgtgtcca ctccgaaggg
1681 gcctgaggaa caggattacc cctacgagag caacacagag gacagcaact tcctcaagcc
1741 actcggagac tgggagagaa gagtgaagag tgaagctgga gcgggaagca gaacccagac
1801 tgaatacgac tacatagacg gggaaggcga ctggagtgct tggtctccat gcagtgtatc
1861 ctgtggaaat ggtaaccaga agcggaccag atcgtgcggt tatgcctgca ctgccacaga
1921 gtcacggaca tgtgacatgc ccagctgtcc agggattgaa gatgcattca agacggcagc
1981 aactgaagtc agtttgctcg ctggcactga agagttcaat gcaacagagc tgtttggagt
2041 tgataccgac agctgtgagc gttggatgaa ctgcaagagt gagtttctca agaaatacat
2101 gagcaaagta gccactgatc tacccagctg cccctgcttt tacccaaccg aggtggccta
2161 cagcaccgca gacgtccacg atgccaacac caaacgcaac ttccgctgga aggacgccag
2221 cgggccaaaa gagaagctgg agatctataa acccacagcc cgctattgca tccgctccat
2281 gctcacgctg gaatccacaa cgttggcggc ccagcactgc tgctacgacg acagcatgaa
2341 actcatcacc cgaggcaaag gagccggtac gccaaatctt atcagcaccg agttctccgc
2401 tgacctacac tacaaagtgg acatcctacc ctggatcatc tgcaaggggg actggagccg
2461 ctacaaccac gctcgacccc ccaataatgg acagaaatgt gcagaaaacc ctcaggatga
2521 ggactactat aaacagtttg aagaggcccg ggagttttga acactatgta ctttcgaaaa
2581 acagaagcag aacttgtgtt gaaagctggc tgtgcgtttg aagaggactt gctataagaa
2641 acagacagca ccgtcaacaa tgcgcagaga tcttcatatg gtctccctca aaaccttggg
2701 tttaaaagtg gaaaatccat catggttctc tttgtattat gtatattatt tggggaatct
2761 ctaaattgtt tgaatttttt aaggaataaa gggaaagaat gaaagggaag tcatatcagg
2821 aaatcaccaa tccatcaatg atcaaccaac agcaagagtt tcccaaattt ttgcatgctt
2881 gtattacact taacatgcag ttggaaaccc ttctcctcag gactctccaa caggacattg
2941 gatacaacat tcaggttctt gtgtaaacat gcgtatattt gtctagctga atgaacagaa
3001 ttcccatata atgtcctgtt gtatcacggt tccgttttta ctaagaacaa aacagccctg
3061 tgtaatcttt gcaaagtatt tattaagtca tagttatgtg tcagtggtag ggatttgtgc
3121 atctagatgt atggacagat tttgagtgct aaataaagag acccatgtca catttaaaaa
3181 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
3241 gggcggccgc tctagaggat ccaagcttac gtacgcgtgc atgcgacgtc atagctcttc
3301 tatagtgtca cctaaattca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
3361 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
3421 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
3481 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
3541 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
3601 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
3661 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
3721 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
3781 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
3841 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
3901 taacgcgaat tttaacaaaa tattaacgtt tacaatttca ggtggcactt ttcggggaaa
3961 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
4021 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
4081 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
4141 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
4201 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
4261 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
4321 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
4381 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
4441 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
4501 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
4561 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
4621 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
4681 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
4741 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
4801 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
4861 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
4921 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
4981 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
5041 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
5101 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
5161 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
5221 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
5281 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
5341 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
5401 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
5461 ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg
5521 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
5581 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
5641 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
5701 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
5761 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
5821 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
5881 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagagctt gcaattcgcg
5941 cgcgaaggcg aagcggcatt tacgttgaca ccatcgaatg gcgcaaaacc tttcgcggta
6001 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt
6061 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
6121 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
6181 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
6241 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
6301 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
6361 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgggt cagtgggctg atcattaact
6421 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
6481 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
6541 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
6601 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
6661 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt
6721 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
6781 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
6841 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
6901 taaccaccat ca
//