clone 23f17 from the microarray screen 1st round (Matthew clark normalized late somitogenesis / 24 hpf / liver library). noncoding, repetitive transcript. similar and overlapping with many other regions in the genome. for antisense probe synthesis.
expressed in paraxial and ventral mesoderm during gastrulation. later in specific brain regions.
for antisense: EcoRI, SP6
LOCUS Gil301.pSP 6347 bp DNA circular 25-MAR-2005
DEFINITION Complementary copy of pSPORT1 23f17.
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT VNTDATE|369928678|
COMMENT VNTDBDATE|369928714|
COMMENT VNTNAME|Gil301.pSPORT1 23f17|
COMMENT VNTAUTHORNAME|Gilbert Weidinger|
COMMENT VNTAUTHOREML|gweidin@u.washington.edu|
FEATURES Location/Qualifiers
promoter complement(2465..2481)
/vntifkey="30"
/label=SP6
promoter 90..106
/vntifkey="30"
/label=T7
CDS 3234..4094
/vntifkey="4"
/label=AmpR
misc_feature 156..2406
/vntifkey="21"
/label=23f17\cDNA
rep_origin complement(2644..3104)
/vntifkey="33"
/label=f1\ori
BASE COUNT 1761 a 1434 c 1465 g 1683 t 4 others
ORIGIN
1 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
61 acagctatga ccatgattac gccaagctct aatacgactc actataggga aagctggtac
121 gcctgcaggt accggtccgg aattcccggg tcgacgccta accagatcag cccataacat
181 ggggtctgcc ccatgttatg tatatttcaa agtcaggaat gcatcttttt catcttggat
241 ttatctttga taatttgatg ttttgtattg atcatttatg aattgactga ttgaattggc
301 ataatacatt tacctgacta ataaattgta atgtttgacc atattttgct cactctgtaa
361 ttattaattc aagtagatta cgctgtatcc ttgtttccgc acgtcttgcg tcaggtcagt
421 agacggacta aaatccaatt gagatggagc attggcacac taattgtgtt ttagcgatcc
481 ggctgactct tgatattgat tcatgtgtac ttaggtggaa agggcgataa cttccgtcta
541 ggtcaacaag gtgttgcacg actaacctag attgggtacc cgacctttgt ttgaaggtaa
601 tattattcta aattaagttc atatgggaaa ccatgggctt ggtgaaaacc aaagttacta
661 tagagtccag tagtcgggta catttatatt aatgccctaa cctctaatta gaaatgatga
721 ttgtcttaac tcaaaggtgt acatctaagc gggactaact aaagtatttt agaacgagcg
781 cgctggtagc ggccatctaa agtattagtc aatttacctc tgtttaatat tcaagactga
841 ccggagtgtg accgtgaggg acgccttcgg ccgaggcgat ttctctgagc gacatgagca
901 cccgaatgaa cggatgtaat agttactagt gaagacaaaa ggttcaaaca tttatctaaa
961 ttacatattg atataatctt cttaatgttt tataattgga gtcagataaa acgaggataa
1021 atatattaat attctaacca ttctcatatt aaaattggag tcagatatga gttaagttta
1081 atataaatca acattgtgca ctggaaagac cgaacatgca gtgaaccttc atccaccatt
1141 ggataccgtc attggaccaa ctgggtggac cttacaaaat ggtgacccat ctccgtgatc
1201 tcccaactca ggcgagttac gtctttccag cacaaaatgg atttatatca accaagaggt
1261 ttggatcttc tattctatct tcagctgctg tggtaagtca attcttcttt gcctattaga
1321 aatgttgagg gaaagctgga cgcaccctwt ataaacacat tgatagaatg agtcggaaat
1381 gctcaaaaat gttagaccgg aatgcgttat ccggtggggt tgagaattag acaaaaccac
1441 tttgattatc agggacatga tagcagcagt atagawaaay gccatccctt gctctgaaat
1501 atctctttgt ctgctaggca gcaagagttg aagcttaaaa ttggaataac atcatccaaa
1561 ttatattctg gractcagtg gagagtcaca aattagatcc taaaagtttg aatcaaaaat
1621 atccattgtt tttaaatcta ctgttttata aaaaagagaa aaatggaaaa aaggaaaaca
1681 ggaaaaaatg gtttaagatt taaaaacaat tgtaaacttt taaattggat taataacaac
1741 agtctgttta aaggcatttg catgtgaaaa ataaaggtga gcagaacaga atgatttgat
1801 tgcaaaaaag acaaataagc tctaatgaaa actctgcttt ttacaaatca gaaatttgcc
1861 atttaaattt aatggggata aattcaaata tgatgctgat aaattaaaat atccacaata
1921 ctttaattta atctcgaaac agactaaata taaaagattt tagctttaaa taagccctta
1981 gacctcctga tggctcaaac cttttcatat tcaaatggcc tgacagagag caaagaagtc
2041 tcacctcagt gataataaga tgctcagaat gtgtttgctg taattgcata aaacaatgca
2101 ctgtgttcta aaacactctc cagagaccag atataattat atttaataca tactatttta
2161 atataatata attacattag gaataaacaa taatgtacat tatattataa tcgccaatca
2221 tggcactaaa gactagagat gaaacattca ttttgaggag gtttgctttc tttatgctta
2281 atgaaaacac tgacagaact actatagaat atctgattgt aaaccaatat aatcctcgct
2341 gaaaaaacac cacctacaat taaaatttga tcagagagtt aaactcacca aaaaaaaaaa
2401 aaaagggcgg ccgctctaga ggatccaagc ttacgtacgc gtgcatgcga cgtcatagct
2461 cttctatagt gtcacctaaa ttcaattcac tggccgtcgt tttacaacgt cgtgactggg
2521 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
2581 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
2641 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
2701 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
2761 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
2821 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
2881 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
2941 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
3001 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
3061 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcaggtggc acttttcggg
3121 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
3181 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
3241 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
3301 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
3361 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
3421 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
3481 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
3541 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
3601 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
3661 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
3721 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
3781 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
3841 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
3901 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
3961 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
4021 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
4081 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
4141 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
4201 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
4261 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
4321 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
4381 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
4441 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
4501 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
4561 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
4621 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg
4681 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
4741 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
4801 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
4861 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
4921 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
4981 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
5041 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcaga gcttgcaatt
5101 cgcgcgcgaa ggcgaagcgg catttacgtt gacaccatcg aatggcgcaa aacctttcgc
5161 ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta
5221 acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg
5281 aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag
5341 ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt
5401 ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa
5461 tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc
5521 gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gggtcagtgg gctgatcatt
5581 aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg
5641 gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa
5701 gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg
5761 ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat
5821 ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc
5881 ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt
5941 gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt
6001 ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg
6061 ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg
6121 ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg
6181 aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
6241 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
6301 aattaatgtg agttagctca ctcattaggc accccaggct ttacact
//