zebrafish Tcf3a (more or less ORF only) in pCS2+. cDNA cut DraI, which includes 11bp of 5' UTR and 87bp of 3' UTR, cloned into StuI site of pCS2+. antisense probe synthesized from this plasmid works. From Chris who got it from Rich. for sense: Asp718, SP6 for antisense: EcoRI, T7