Probe Construct Name: Nlk1-pGEM-T

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Plasmid size: 1425bp coding region, 62bp 5'UTR, and 130bp 3'UTR.

cDNA is cloned in pGEM-T. For antisense probe cut with SacII and transcribe with SP6. For sense probe cut with Sal1 and transcribe with T7.


Plasmid History
Species of Sequence Origin: Zebrafish
Citation: C. J. Thorpe and R. T. Moon (2004). Development 131:2899-2909.