Plasmid size:
Full length prickle-1 with 42bp of 5' UTR, cloned into pGEM-Teasy. The construct makes good in situ probes but does not translate in vitro due to an atg in the polylinker.
To make antisense probe: cut with Nco1, transcribe with SP6. For the sense strand cut with Nde1 and transcribe with T7.