Probe Construct Name: pwnt8 3'orf1

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Plasmid size:

530 bp PCR product igated into vector pGEMTeasy. Purpose of clone is to provide an in situ probe specific for the first open reading frame of the bicistronic wnt8 RNA.

To make antisense probe: cut with Sph1, transcribe with SP6.



Plasmid History
Species of Sequence Origin: Zebrafish
Citation: A. Lekven et al., 2001, Developmental Cell.