Vector: pBluescript
Insert: pax6 cDNA subclone
Antisense probe cut with Xba 1 and transcribe with T3.
Notes for Use:
Provided by Jeremy Wegener (Monte Westerfield's lab).
pA25 is the second subclone they use, mixing both probes with this.
T3 doesn't transcribe too well.
Citation: Development 114, 643-651.