pSPORT1 Sp5-l
Gilbert Weidinger, Ph.D.
Zebrafish sp5-like (sp5l) cDNA (likely full-length) in pSPORT1.
Clone 27i2 from the microarray screen 1st round (Matthew clark normalized late
somitogenesis / 24 hpf / liver library).
Expressed strongly in broad marginal region during gastrulation.
Everywhere except head at bud, then rapidly declines to stay in tailbud and lateral
mesoderm (or edge of neural plate).
For antisense: Asp718, SP6
gcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctg
cgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccat
gtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatgg
cgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgatacc
gaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccg
cttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccct
ggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactgga
aagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggc
tcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagctctaata
cgactcactatagggaaagctggtacgcctgcaggtaccggtccggaattcccgggtcgacggacttattgcgacaggccct
tcagtcgcacttcaaaaggaagagacctggtttgaaagacgctgcttttgaaaacgagacccagaaaaacaggagcaacagc
gcggagtggccaacaactctcacaggcgagaaaggacaggatttggaagagagaatccaaggattattgatttttcagcgat
atatctgaacgtacgcacctggctgtgtaagggggtcaacaggatctttgcgcgcctcctcgttcccgcacactgagacttt
ggcacggctacgcaccgcgcgccgcgcttcacgcaggtgtggatatcttgaaacaaccgtgaacacatggctgcattaacct
tatccagagcggacaatttcctccacaatttcttacaggaccgcactcccagctcatctccagaaagtggtccaaacaccct
ctcctttctggccaccacatgcagtcaggcttggcaggaaggctcgcagctcccttacgagggtccggtgggctccgcatcc
gcatgttccagctctggagcaacgacgtggccccgaactccagcttaagcgctcaccagatgaccttcaccgtgcccaaaat
gcagtttcccagccacatgcaacccactctgggctcgcactcccatcaccaccatcaccaccatcatcaccaccacgagctt
cccctcactcctccagcggagcctccgtctgcatattcattcgagctttccccggttaaaatgcaagggaatgcgccatacta
cacgcagcacaacgccgtcagtcaaaactttccaagcttcctccaaaacccctcaggaagagctcacctgcctggaggtcacg
ttgaggatggccagcagtggtggagtctcccccagggtaacagcgctccctccgggcaccctttctccttgggacggcagctg
gttttgggccatcagcctcaaattgcagcacttctgcagggaacctccaaaggtctgttgagctctactcgtcgctgtcgccga
tgcaaatgcccaaactgccagtccacaggaaacggaggtgctgcattggagtttggaaagaaaagactacacatttgtcacatt
ccagattgtgggaaggtttataaaaaaacctctcacctcaaagcgcacctgcgctggcatgctggtgaacggcccttcatttgc
aactggctcttctgcgggaagagcttcacgcgttcagatgagctgcagaggcatctccgtacacacaccggggagaagcgtttcg
gctgtcagcagtgtggaaaacggtttatgcggagcgatcatctctccaagcatgtgaagacccatcagagccggaagagccggtc
cagtcagcctcctcacagcgggactgatgctctgctgagcaacatcaagagagagtaaaagatgcacaagtactaaaaacaata
tgaacaagagctaagagagagaacttttttagagtctggaagtttggcagtggtgaaatggttgcgatgggagcatgggcctgt
ttctactactgattgtttcctgaaaacactggagtaaagtgctgcaccaaaactgatgttataaagactgtaaagagacttcag
ggcaaagcacaagtggtgtgcgagaggcattttagtatgcctcatgcccttgcacatttatttaaagacaatttaattcaaaatg
agaaaaaagtattcgttatttggtgaaactgtccttcctttgtttacattctgttgtttgttttctctattttgctttagggaaaa
ttgatgccttatgttgaaatcctgaattcagaacatttgccaactaaattcttgttttcttgcagagaatggcactgctatattc
actctaaatccctatagtttcaaaacaagaaccaaatgtatataatgtttatatagcgcacagttctataaagaccagcatgtgtaa
taataataataaacacgatcgactgtgcaaatcaaaaaaaaaaaaaaagggcggccgctctagaggatccaagcttacgtacgcgtg
catgcgacgtcatagctcttctatagtgtcacctaaattcaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctgg
cgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttccca
acagttgcgcagcctgaatggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgac
cgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagct
ctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgt
agtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactgg
aacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgat
ttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggcacttttcggggaaatgtgcgcggaacccct
atttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaagga
agagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctg
gtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttt
tcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagca
actcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaaga
gaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgct
tttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacacca
cgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggat
ggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtggg
tctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatga
acgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatt
taaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccact
gagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgc
taccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtcc
ttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctg
ccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgca
cacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagcattgagaaagcgccacgcttcccgaagggagaaagg
cggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgg
gtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacgg
ttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctg
ataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgt
tggccgattcattaatgcagagcttgcaattcgcgcgcgaaggcgaagcggcatttacgttgacaccatcgaatggcgcaaaacctttcgcggta
tggcatgatagcgcccggaagagagtcaattcagggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttat
cagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcc
caaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcg
cggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcaca
atcttctcgcgcaacgggtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccgg
cgttatttcttga