RETURN TO ZEBRAFISH EXPRESSION LIST
Description: zebrafish sp5-like (sp5l) zinc-finger transcription factor of the SP5 family (cDNA clone 27i2 from the Wnt8 upregulated microarray screen) ORF only in pCS2P+. PCR amplified using primers 96 &97 from 27-i2 (pSPORT) clone and cloned EcoRI/XbaI into pCS2P+. Injection of high doses (>260 ng/µl corresponding to 200ng/µl #53.GFP) results in loss of eyes.
Notes for Use: For functional synthetic RNA, linearize with NotI, SP6 (1385bp RNA). Select clones with amp.
Reference: Development, Current Biology papers from the lab (2005).
LOCUS Gil156.pCS 5168 bp DNA circular 3-JUL-2003
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT VNTDATE|290544468|
COMMENT VNTDBDATE|307208401|
COMMENT VNTNAME|Gil156.pCS2P+27-i2 ORF|
COMMENT VNTAUTHORNAME|Demo User|
FEATURES Location/Qualifiers
mRNA 1..1
/vntifkey="54"
/label=cap\site\of\CMV\prom
/note="base1 = cap site for sCMV IE94 promoter"
CDS complement(2664..3524)
/vntifkey="4"
/label=AmpR
/note="beta-lactamase"
promoter 35..51
/vntifkey="30"
/label=SP6
/note="SP6 promoter"
5'UTR <64..78
/vntifkey="52"
/label=ßglobin\5'\RNA
/note="B-globin 5' mRNA sequence"
promoter complement(1196..1212)
/vntifkey="30"
/label=T7
/note="T7 promoter (reverse orientation)"
polyA_site 1217..1412
/vntifkey="26"
/label=SV40\pA
/note="SV40 late polyadenylation site (Bam to Dra1)"
promoter complement(1466..1482)
/vntifkey="30"
/label=T3
/note="T3 promoter (reverse orientation)"
rep_origin 3653..4113
/vntifkey="33"
/label=f1\ori
/note="f1 ori"
enhancer 4182..>5168
/vntifkey="9"
/label=CMV\enh/prom
/note="simian CMV IE94 enhancer/promoter"
misc_feature 105..107
/vntifkey="21"
/label=first\ATG
misc_feature 146..158
/vntifkey="21"
/label=27i2\spMO\half\binding\site
misc_feature 942..1016
/vntifkey="21"
/label=pfam00096,\zf-C2H2,\Zinc\finger,\C2H2\type
CDS 105..1178
/vntifkey="4"
/label=SP-like\transcription\factor
BASE COUNT 1300 a 1337 c 1258 g 1273 t
ORIGIN
1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
61 ctacttgttc tttttgcagg atcccatcga ttcgaattcc caccatggct gcattaacct
121 tatccagagc ggacaatttc ctccacaatt tcttacagga ccgcactccc agctcatctc
181 cagaaagtgg tccaaacacc ctctcctttc tggccaccac atgcagtcag gcttggcagg
241 aaggctcgca gctcccttac gagggtccgg tgggctccgc atccagcatg ttccagctct
301 ggagcaacga cgtggccccg aactccagct taagcgctca ccagatgacc ttcaccgtgc
361 ccaaaatgca gtttcccagc cacatgcaac ccactctggg ctcgcactcc catcaccacc
421 atcaccacca tcatcaccac cacgagcttc ccctcactcc tccagcggag cctccgtctg
481 catattcatt cgagctttcc ccggttaaaa tgcaagggaa tgcgccatac tacacgcagc
541 acaacgccgt cagtcaaaac tttccaagct tcctccaaaa cccctcagga agagctcacc
601 tgcctggagg tcacgttgag gatggccagc agtggtggag tctcccccag ggtaacagcg
661 ctccctccgg gcaccctttc tccttgggac ggcagctggt tttgggccat cagcctcaaa
721 ttgcagcact tctgcaggga acctccaaag gtctgttgag ctctactcgt cgctgtcgcc
781 gatgcaaatg cccaaactgc cagtccacag gaaacggagg tgctgcattg gagtttggaa
841 agaaaagact acacatttgt cacattccag attgtgggaa ggtttataaa aaaacctctc
901 acctcaaagc gcacctgcgc tggcatgctg gtgaacggcc cttcatttgc aactggctct
961 tctgcgggaa gagcttcacg cgttcagatg agctgcagag gcatctccgt acacacaccg
1021 gggagaagcg tttcggctgt cagcagtgtg gaaaacggtt tatgcggagc gatcatctct
1081 ccaagcatgt gaagacccat cagagccgga agagccggtc cagtcagcct cctcacagcg
1141 ggactgatgc tctgctgagc aacatcaaga gagagtaatc tagattctgc agccctatag
1201 tgagtcgtat tacgtagatc cagacatgat aagatacatt gatgagtttg gacaaaccac
1261 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
1321 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
1381 tcaggttcag ggggaggtgt gggaggtttt ttaattcgcg gccgcggcgc caatgcattg
1441 ggcccggtac ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
1501 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
1561 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
1621 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
1681 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
1741 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
1801 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
1861 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
1921 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
1981 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
2041 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
2101 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
2161 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
2221 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
2281 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
2341 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
2401 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
2461 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
2521 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
2581 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
2641 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
2701 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
2761 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
2821 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
2881 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
2941 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
3001 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
3061 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
3121 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
3181 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
3241 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
3301 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
3361 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
3421 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
3481 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
3541 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
3601 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
3661 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
3721 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
3781 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
3841 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
3901 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
3961 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
4021 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
4081 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
4141 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gtcgaccata gccaattcaa
4201 tatggcgtat atggactcat gccaattcaa tatggtggat ctggacctgt gccaattcaa
4261 tatggcgtat atggactcgt gccaattcaa tatggtggat ctggacccca gccaattcaa
4321 tatggcggac ttggcaccat gccaattcaa tatggcggac ttggcactgt gccaactggg
4381 gaggggtcta cttggcacgg tgccaagttt gaggaggggt cttggccctg tgccaagtcc
4441 gccatattga attggcatgg tgccaataat ggcggccata ttggctatat gccaggatca
4501 atatataggc aatatccaat atggccctat gccaatatgg ctattggcca ggttcaatac
4561 tatgtattgg ccctatgcca tatagtattc catatatggg ttttcctatt gacgtagata
4621 gcccctccca atgggcggtc ccatatacca tatatggggc ttcctaatac cgcccatagc
4681 cactccccca ttgacgtcaa tggtctctat atatggtctt tcctattgac gtcatatggg
4741 cggtcctatt gacgtatatg gcgcctcccc cattgacgtc aattacggta aatggcccgc
4801 ctggctcaat gcccattgac gtcaatagga ccacccacca ttgacgtcaa tgggatggct
4861 cattgcccat tcatatccgt tctcacgccc cctattgacg tcaatgacgg taaatggccc
4921 acttggcagt acatcaatat ctattaatag taacttggca agtacattac tattggaagg
4981 acgccagggt acattggcag tactcccatt gacgtcaatg gcggtaaatg gcccgcgatg
5041 gctgccaagt acatccccat tgacgtcaat ggggaggggc aatgacgcaa atgggcgttc
5101 cattgacgta aatgggcggt aggcgtgcct aatgggaggt ctatataagc aatgctcgtt
5161 tagggaac
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