Expression Construct Name: Nlk1(K117M)-pCS2+

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Description: Zebrafish Nemo-like kinase 1(NLK-1), cloned into the vector CS2+. ~1425bp EcoR1-Xba1 insert. Clone is an kinase dead mutant of NLK1, analogous to K155M mutant of mouse NLK.



Species of Sequence Origin: zebrafish

Notes for Use: For functional synthetic RNA, linearize with Asp 718 and transcribe with SP6. Select clones with amp.

Reference: C. J. Thorpe and R. T. Moon, Development 131:2899-2909 (2004).