Expression Construct Name: wnt8 pBand3-5
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Vector: pGemTeasy
RT PCR prodct ligated into vector full length cDNA cloned includes ORF1, IRES and ORF2.
For antiense RNA: transcribe with SP6 polymerase.
Plasmid History
Species of Sequence Origin: Zebrafish
Reference: Lekven, December, Cell, 2001.